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Literature summary for 1.1.1.179 extracted from

  • Berghaell, S.; Hilditch, S.; Penttilae, M.; Richard, P.
    Identification in the mould Hypocrea jecorina of a gene encoding an NADP(+):D-xylose dehydrogenase (2007), FEMS Microbiol. Lett., 277, 249-253.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.179

Cloned(Commentary)

Cloned (Comment) Organism
gene xyd1, DNA and amino acid sequence determination and analysis, functional expression of the His-tagged enzyme in Saccharomyces cerevisiae Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, recombinant enzyme Trichoderma reesei
5.7
-
 D-glucose pH 8.0, 30°C, recombinant enzyme Trichoderma reesei
24
-
 NADP+ pH 8.1, 30°C, recombinant enzyme Trichoderma reesei
43
-
 D-xylose pH 8.1, 30°C, recombinant enzyme Trichoderma reesei

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Trichoderma reesei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42720
-
 x * 42720, sequence calculation Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NADP+ Trichoderma reesei best substrate D-xylono-1,5-lactone + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei A8BT09 i.e. Trichoderma reesei, strain VTT-D-80133, gene xyd1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Saccharomyces cerevisiae by nickel affinity chromatography Trichoderma reesei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + NADP+
-
 Trichoderma reesei D-galactono-1,5-lactone + NADPH
-
 ?
D-glucose + NADP+
-
 Trichoderma reesei D-glucono-1,5-lactone + NADPH + H+
-
 ?
D-ribose + NADP+ low activity Trichoderma reesei ? + NADPH
-
 ?
D-xylose + NADP+ best substrate Trichoderma reesei D-xylono-1,5-lactone + NADPH + H+
-
 ?
L-arabinose + NADP+
-
 Trichoderma reesei L-arabinono-1,4-lactone + NADPH
-
 ?
additional information no or poor activity with D-arabinose, D-mannose, L-rhamnose, D-lyxose, D-fructose, D-glyceraldehyde, and DL-glyceraldehyde Trichoderma reesei ?
-
 ?

Subunits

Subunits Comment Organism
? x * 42720, sequence calculation Trichoderma reesei

Synonyms

Synonyms Comment Organism
NADP+-dependent D-xylose dehydrogenase
-
 Trichoderma reesei
NADP+:D-xylose dehydrogenase
-
 Trichoderma reesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Trichoderma reesei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.7
-
 D-glucose pH 8.0, 30°C, recombinant enzyme Trichoderma reesei
21.8
-
 D-xylose pH 8.1, 30°C, recombinant enzyme Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
 assay at Trichoderma reesei

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on, specific for Trichoderma reesei