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Literature summary for 1.1.1.203 extracted from

  • Pick, A.; Schmid, J.; Sieber, V.
    Characterization of uronate dehydrogenases catalysing the initial step in an oxidative pathway (2015), Microb. Biotechnol., 8, 633-643.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Streptomyces viridochromogenes
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas syringae pv. tomato
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Fulvimarina pelagi
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Oceanicola granulosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Streptomyces viridochromogenes
additional information
-
additional information Michaelis-Menten kinetics Pseudomonas syringae pv. tomato
additional information
-
additional information Michaelis-Menten kinetics Agrobacterium tumefaciens
additional information
-
additional information Michaelis-Menten kinetics Fulvimarina pelagi
additional information
-
additional information Michaelis-Menten kinetics Oceanicola granulosus
0.02
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Fulvimarina pelagi
0.03
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Fulvimarina pelagi
0.03
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Pseudomonas syringae pv. tomato
0.04
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Pseudomonas syringae pv. tomato
0.1
-
NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Streptomyces viridochromogenes
0.1
-
NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Agrobacterium tumefaciens
0.1
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Agrobacterium tumefaciens
0.14
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Pseudomonas syringae pv. tomato
0.2
-
NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Pseudomonas syringae pv. tomato
0.2
-
NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Fulvimarina pelagi
0.2
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Streptomyces viridochromogenes
0.2
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Agrobacterium tumefaciens
0.2
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Fulvimarina pelagi
0.3
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Streptomyces viridochromogenes
0.3
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Fulvimarina pelagi
0.3
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Agrobacterium tumefaciens
0.4
-
NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Oceanicola granulosus
0.5
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Pseudomonas syringae pv. tomato
1
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Agrobacterium tumefaciens
6.4
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Fulvimarina pelagi
7.3
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Pseudomonas syringae pv. tomato
8.3
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Agrobacterium tumefaciens
9
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Streptomyces viridochromogenes
9.5
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Fulvimarina pelagi
13.6
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Streptomyces viridochromogenes
19.4
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Agrobacterium tumefaciens
22.05
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Pseudomonas syringae pv. tomato
55
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Oceanicola granulosus
59
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Oceanicola granulosus
64
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Oceanicola granulosus
64
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Oceanicola granulosus
112
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Oceanicola granulosus
142
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Oceanicola granulosus
465
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Streptomyces viridochromogenes
628
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Streptomyces viridochromogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-galacturonate + NAD+ Streptomyces viridochromogenes
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+ Pseudomonas syringae pv. tomato
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+ Agrobacterium tumefaciens
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+ Fulvimarina pelagi
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+ Oceanicola granulosus
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+ Oceanicola granulosus DSM 15982
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+ Streptomyces viridochromogenes DSM 40736
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+ Pseudomonas syringae pv. tomato DC3000
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+ Agrobacterium tumefaciens C58 / ATCC 33970
-
D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Streptomyces viridochromogenes
-
D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Pseudomonas syringae pv. tomato
-
D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Agrobacterium tumefaciens
-
D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Fulvimarina pelagi
-
D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Oceanicola granulosus
-
D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Oceanicola granulosus DSM 15982
-
D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Streptomyces viridochromogenes DSM 40736
-
D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Pseudomonas syringae pv. tomato DC3000
-
D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+ Agrobacterium tumefaciens C58 / ATCC 33970
-
D-glucaro-1,5-lactone + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens Q7CRQ0
-
-
Agrobacterium tumefaciens C58 / ATCC 33970 Q7CRQ0
-
-
Fulvimarina pelagi
-
-
-
Oceanicola granulosus
-
-
-
Oceanicola granulosus DSM 15982
-
-
-
Pseudomonas syringae pv. tomato Q888H1
-
-
Pseudomonas syringae pv. tomato DC3000 Q888H1
-
-
Streptomyces viridochromogenes
-
-
-
Streptomyces viridochromogenes DSM 40736
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-galacturonate + NAD+
-
Streptomyces viridochromogenes D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+
-
Pseudomonas syringae pv. tomato D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+
-
Agrobacterium tumefaciens D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+
-
Fulvimarina pelagi D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+
-
Oceanicola granulosus D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+
-
Oceanicola granulosus DSM 15982 D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+
-
Streptomyces viridochromogenes DSM 40736 D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+
-
Pseudomonas syringae pv. tomato DC3000 D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-galacturonate + NAD+
-
Agrobacterium tumefaciens C58 / ATCC 33970 D-galactaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Streptomyces viridochromogenes D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Pseudomonas syringae pv. tomato D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Agrobacterium tumefaciens D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Fulvimarina pelagi D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Oceanicola granulosus D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Oceanicola granulosus DSM 15982 D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Streptomyces viridochromogenes DSM 40736 D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Pseudomonas syringae pv. tomato DC3000 D-glucaro-1,5-lactone + NADH + H+
-
?
beta-D-glucuronate + NAD+
-
Agrobacterium tumefaciens C58 / ATCC 33970 D-glucaro-1,5-lactone + NADH + H+
-
?
D-mannuronate + NAD+
-
Streptomyces viridochromogenes ? + NADH + H+
-
?
D-mannuronate + NAD+
-
Pseudomonas syringae pv. tomato ? + NADH + H+
-
?
D-mannuronate + NAD+
-
Agrobacterium tumefaciens ? + NADH + H+
-
?
D-mannuronate + NAD+
-
Fulvimarina pelagi ? + NADH + H+
-
?
D-mannuronate + NAD+
-
Oceanicola granulosus ? + NADH + H+
-
?
D-mannuronate + NAD+
-
Oceanicola granulosus DSM 15982 ? + NADH + H+
-
?
D-mannuronate + NAD+
-
Streptomyces viridochromogenes DSM 40736 ? + NADH + H+
-
?
D-mannuronate + NAD+
-
Pseudomonas syringae pv. tomato DC3000 ? + NADH + H+
-
?
D-mannuronate + NAD+
-
Agrobacterium tumefaciens C58 / ATCC 33970 ? + NADH + H+
-
?
additional information substrate and cofactor specificity of Udhs, overview Streptomyces viridochromogenes ?
-
?
additional information substrate and cofactor specificity of Udhs, overview Pseudomonas syringae pv. tomato ?
-
?
additional information substrate and cofactor specificity of Udhs, overview Agrobacterium tumefaciens ?
-
?
additional information substrate and cofactor specificity of Udhs, overview Fulvimarina pelagi ?
-
?
additional information substrate and cofactor specificity of Udhs, overview Oceanicola granulosus ?
-
?
additional information substrate and cofactor specificity of Udhs, overview Oceanicola granulosus DSM 15982 ?
-
?
additional information substrate and cofactor specificity of Udhs, overview Streptomyces viridochromogenes DSM 40736 ?
-
?
additional information substrate and cofactor specificity of Udhs, overview Pseudomonas syringae pv. tomato DC3000 ?
-
?
additional information substrate and cofactor specificity of Udhs, overview Agrobacterium tumefaciens C58 / ATCC 33970 ?
-
?

Synonyms

Synonyms Comment Organism
Atu3143
-
Agrobacterium tumefaciens
PSPTO_1053
-
Pseudomonas syringae pv. tomato
Udh
-
Streptomyces viridochromogenes
Udh
-
Pseudomonas syringae pv. tomato
Udh
-
Agrobacterium tumefaciens
Udh
-
Fulvimarina pelagi
Udh
-
Oceanicola granulosus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
-
Oceanicola granulosus
35
-
-
Pseudomonas syringae pv. tomato
45
-
-
Streptomyces viridochromogenes
60
-
-
Agrobacterium tumefaciens
60
-
-
Fulvimarina pelagi

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
15 60 activity range, profile overview Oceanicola granulosus
15 60 activity is constantly increasing within this range, profile overview Agrobacterium tumefaciens
15 60 activity is constantly increasing within this range, profile overview Fulvimarina pelagi
15 50 activity range, inactivation at 55┬░C, profile overview Pseudomonas syringae pv. tomato
15 55 activity range, profile overview Streptomyces viridochromogenes

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
37
-
purified recombinant His-tagged enzyme, 10% activity remaning after 100 min, inactivation within 150 min Agrobacterium tumefaciens
37
-
purified recombinant His-tagged enzyme, 50% activity remaining after 120 min, 25% activity remaining after 300 min Fulvimarina pelagi
37
-
purified recombinant His-tagged enzyme, 50% remaining activity after 1000-1500 min, 30% remaning after 2500 min Oceanicola granulosus
37
-
purified recombinant His-tagged enzyme, inactivation within 50 min Streptomyces viridochromogenes
37
-
purified recombinant His-tagged enzyme, inactivation within 50 min Pseudomonas syringae pv. tomato

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Oceanicola granulosus
3.4
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Oceanicola granulosus
4
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Oceanicola granulosus
4
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Oceanicola granulosus
6
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Oceanicola granulosus
6.4
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Pseudomonas syringae pv. tomato
7
-
NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Oceanicola granulosus
7
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Pseudomonas syringae pv. tomato
7
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Oceanicola granulosus
8
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Streptomyces viridochromogenes
9
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Streptomyces viridochromogenes
10
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Fulvimarina pelagi
13
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Fulvimarina pelagi
17
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Streptomyces viridochromogenes
21
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Pseudomonas syringae pv. tomato
23
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Streptomyces viridochromogenes
29
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Pseudomonas syringae pv. tomato
53
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Streptomyces viridochromogenes
58
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Fulvimarina pelagi
59
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Agrobacterium tumefaciens
62
-
NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Streptomyces viridochromogenes
63
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Pseudomonas syringae pv. tomato
75
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Streptomyces viridochromogenes
76
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Agrobacterium tumefaciens
78
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Fulvimarina pelagi
82
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Fulvimarina pelagi
85
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Agrobacterium tumefaciens
92
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Agrobacterium tumefaciens
107
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Fulvimarina pelagi
108
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Pseudomonas syringae pv. tomato
113
-
NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Fulvimarina pelagi
144
-
NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Agrobacterium tumefaciens
171
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Agrobacterium tumefaciens
215
-
NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Pseudomonas syringae pv. tomato
333
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Oceanicola granulosus
8
-
-
Pseudomonas syringae pv. tomato
8 9
-
Agrobacterium tumefaciens
9
-
-
Fulvimarina pelagi
9.5 10
-
Streptomyces viridochromogenes

pH Range

pH Minimum pH Maximum Comment Organism
5 10 activity range, profile overview Agrobacterium tumefaciens
5 10 activity range, profile overview Fulvimarina pelagi
5 10 activity is constantly increasing in this range, profile overview Streptomyces viridochromogenes
5.5 9.5 activity range, profile overview Pseudomonas syringae pv. tomato
5.5 9.5 activity range, profile overview Oceanicola granulosus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Streptomyces viridochromogenes
NAD+
-
Pseudomonas syringae pv. tomato
NAD+
-
Agrobacterium tumefaciens
NAD+
-
Fulvimarina pelagi
NAD+
-
Oceanicola granulosus
NADH
-
Streptomyces viridochromogenes
NADH
-
Pseudomonas syringae pv. tomato
NADH
-
Agrobacterium tumefaciens
NADH
-
Fulvimarina pelagi
NADH
-
Oceanicola granulosus

General Information

General Information Comment Organism
evolution the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily Streptomyces viridochromogenes
evolution the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily Pseudomonas syringae pv. tomato
evolution the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily Agrobacterium tumefaciens
evolution the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily Fulvimarina pelagi
evolution the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily Oceanicola granulosus
metabolism uronate dehydrogenases catalyse the initial step in an oxidative pathway Streptomyces viridochromogenes
metabolism uronate dehydrogenases catalyse the initial step in an oxidative pathway Pseudomonas syringae pv. tomato
metabolism uronate dehydrogenases catalyse the initial step in an oxidative pathway Agrobacterium tumefaciens
metabolism uronate dehydrogenases catalyse the initial step in an oxidative pathway Oceanicola granulosus
metabolism uronate dehydrogenases catalyses the initial step in an oxidative pathway Fulvimarina pelagi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.03
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Oceanicola granulosus
0.03
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Oceanicola granulosus
0.04
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Oceanicola granulosus
0.07
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Oceanicola granulosus
0.1
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Oceanicola granulosus
0.11
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Oceanicola granulosus
0.3
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Pseudomonas syringae pv. tomato
0.7
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Streptomyces viridochromogenes
0.7
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Streptomyces viridochromogenes
4
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Agrobacterium tumefaciens
9
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Pseudomonas syringae pv. tomato
11
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Fulvimarina pelagi
11
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Agrobacterium tumefaciens
12
-
D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Fulvimarina pelagi
88
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Streptomyces viridochromogenes
98
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Streptomyces viridochromogenes
200
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Agrobacterium tumefaciens
201
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Streptomyces viridochromogenes
210
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Pseudomonas syringae pv. tomato
257
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Streptomyces viridochromogenes
269
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Fulvimarina pelagi
274
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Fulvimarina pelagi
342
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Agrobacterium tumefaciens
406
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Fulvimarina pelagi
430
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Fulvimarina pelagi
465
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Pseudomonas syringae pv. tomato
628
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Pseudomonas syringae pv. tomato
638
-
NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Streptomyces viridochromogenes
646
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Agrobacterium tumefaciens
785
-
NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Fulvimarina pelagi
794
-
beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Pseudomonas syringae pv. tomato
829
-
beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer Agrobacterium tumefaciens
1083
-
NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Agrobacterium tumefaciens
1262
-
NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer Pseudomonas syringae pv. tomato