BRENDA - Enzyme Database show
show all sequences of 1.1.1.391

A biosynthetic pathway for a prominent class of microbiota-derived bile acids

Devlin, A.; Fischbach, M.; Nat. Chem. Biol. 11, 685-690 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene Elen_0198, DNA and amino acid sequence determination and analysis, recombinant overvexpression in Escherichia coli; gene Elen_1325, DNA and amino acid sequence determination and analysis, recombinant overvexpression in Escherichia coli
Eggerthella lenta
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.63
-
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid
pH 7.0, 37°C
Eggerthella lenta
2.66
-
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid
pH 7.0, 37°C
Eggerthella lenta
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
Eggerthella lenta
-
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
-
-
?
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
Eggerthella lenta ATCC 25559
-
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Eggerthella lenta
C8WGQ3
gene Elen_1325
-
Eggerthella lenta
C8WJW0
gene Elen_0198
-
Eggerthella lenta ATCC 25559
C8WGQ3
gene Elen_1325
-
Eggerthella lenta ATCC 25559
C8WJW0
gene Elen_0198
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
-
736859
Eggerthella lenta
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
-
-
-
?
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
-
736859
Eggerthella lenta ATCC 25559
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at; assay at
Eggerthella lenta
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
9.73
-
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid
pH 7.0, 37°C
Eggerthella lenta
29.71
-
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid
pH 7.0, 37°C
Eggerthella lenta
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at; assay at
Eggerthella lenta
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
;
Eggerthella lenta
Cloned(Commentary) (protein specific)
Commentary
Organism
gene Elen_0198, DNA and amino acid sequence determination and analysis, recombinant overvexpression in Escherichia coli
Eggerthella lenta
gene Elen_1325, DNA and amino acid sequence determination and analysis, recombinant overvexpression in Escherichia coli
Eggerthella lenta
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Eggerthella lenta
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.63
-
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid
pH 7.0, 37°C
Eggerthella lenta
2.66
-
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid
pH 7.0, 37°C
Eggerthella lenta
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
Eggerthella lenta
-
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
-
-
?
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
Eggerthella lenta ATCC 25559
-
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
-
736859
Eggerthella lenta
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
-
-
-
?
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
-
736859
Eggerthella lenta ATCC 25559
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Eggerthella lenta
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
9.73
-
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid
pH 7.0, 37°C
Eggerthella lenta
29.71
-
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid
pH 7.0, 37°C
Eggerthella lenta
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Eggerthella lenta
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the biosynthetic pathway for the second most abundant class in the gut, 3beta-hydroxy(iso)bile acids; the enzyme is involved in the biosynthetic pathway for the second most abundant class in the gut, 3beta-hydroxy(iso)bile acids
Eggerthella lenta
physiological function
the iso-bile acid pathway detoxifies deoxycholic acid and thus favors the growth of the keystone genus Bacteroides; the iso-bile acid pathway detoxifies deoxycholic acid and thus favors the growth of the keystone genus Bacteroides
Eggerthella lenta
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the biosynthetic pathway for the second most abundant class in the gut, 3beta-hydroxy(iso)bile acids
Eggerthella lenta
physiological function
the iso-bile acid pathway detoxifies deoxycholic acid and thus favors the growth of the keystone genus Bacteroides
Eggerthella lenta
Other publictions for EC 1.1.1.391
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736859
Devlin
A biosynthetic pathway for a p ...
Eggerthella lenta, Eggerthella lenta ATCC 25559
Nat. Chem. Biol.
11
685-690
2015
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1
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2
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4
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4
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4
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1
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2
1
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1
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2
2
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2
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4
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4
-
2
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2
2
-
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2
4
-
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736216
Marschall
Human liver class I alcohol de ...
Homo sapiens
Hepatology
31
990-996
2000
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1
-
-
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4
1
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1
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2
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1
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1
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6
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1
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4
1
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2
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1
2
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4
1
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1
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1
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1
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6
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1
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4
1
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1
1
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4
4
286228
Edenharder
Characterization of NAD-depend ...
Blautia producta, Blautia producta K2/25-1
Biochim. Biophys. Acta
1004
230-238
1989
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-
-
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1
1
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1
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1
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2
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1
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8
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6
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1
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3
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1
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1
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1
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8
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6
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1
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3
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1
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735471
Edenharder
Partial purification and chara ...
Clostridium innocuum, Clostridium innocuum D1144/3
Appl. Environ. Microbiol.
55
1656-1659
1989
-
-
-
-
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1
1
1
1
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1
4
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2
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1
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1
1
1
11
1
1
-
1
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1
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1
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1
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1
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1
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1
1
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1
4
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1
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1
1
1
11
1
1
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1
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1
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1
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1
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