Application | Comment | Organism |
---|---|---|
medicine | the sensitizing effect of IDPm siRNA and autophagy inhibitor on the ionizing radiation induced apoptotic cell death of glioma cells offers a novel redox-active therapeutic strategy for the treatment of cancer | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene IDPm, reverse transcription-PCR expression analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme IPDM silencing by siRNA in A-172 cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P48735 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-172 cell | - |
Homo sapiens | - |
glioma cell | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
IDPm | - |
Homo sapiens |
mitochondrial NADP+-dependent isocitrate dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | autophagic response to ionizing radiation in A-172 glioma cells transfected with small interfering RNA (siRNA) targeting the IDPm gene. Autophagy in A-172 transfectant cells is associated with enhanced autophagolysosome formation and GFP-LC3 punctuation/aggregation. The inhibition of autophagy by chloroquine augments apoptotic cell death of irradiated A-172 cells transfected with IDPm siRNA. The activity of cytosolic NADP+-dependent isocitrate dehydrogenase (IDPc) is not affected by the transfection of IDPm siRNA. The decreased activity caused by knockdown of IDPm siRNA is also observed in U87MG glioma cell line. When cultured A172 cells are treated with gamma-radiation, an increase in cell death is observed, but A172 cells transfected with IDPm siRNA are significantly more sensitive than control cells in this respect | Homo sapiens |