Application | Comment | Organism |
---|---|---|
biotechnology | the icdA gene is a potentially valuable tool for modulating citric acid production by metabolic engineering | Aspergillus niger |
Cloned (Comment) | Organism |
---|---|
icdA, recombinant overexpression in Aspergillus niger strain WU-2223L | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an NADP+-ICDH gene (icdA)-overexpressing strain (OPI-1) using Aspergillus niger strain WU-2223L. The amount of citric acid produced by Aspergillus niger can be altered with the NADP+-ICDH activity. Time-dependent transcriptional changes in the genes encoding NADP+-ICDH, NAD+-ICDH, and ICL are analyzed by RT-PCR. The glyoxylate cycle is poorly activated or slightly repressed in the icdA-overexpressing Aspergillus niger clone | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Aspergillus niger | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus niger | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Aspergillus niger | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
icdA | - |
Aspergillus niger |
NADP+-ICDH | - |
Aspergillus niger |
NADP+-specific isocitrate dehydrogenase | - |
Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Aspergillus niger | |
NADPH | - |
Aspergillus niger |
General Information | Comment | Organism |
---|---|---|
metabolism | in the tricarboxylic acid cycle, NADP+-specific isocitrate dehydrogenase catalyzes oxidative decarboxylation of isocitric acid to form 2-oxoglutaric acid with NADP+ as a cofactor. NADP+-ICDH may work independently of NAD+-ICDH, EC 1.1.1.41, as one of the TCA cycle regulatory enzymes, especially in relation to citric acid production by Aspergillus niger | Aspergillus niger |
physiological function | ICDH is a key enzyme that regulates the TCA cycle | Aspergillus niger |