KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
NADPH | moderately ethanol producing strain, pH 7.0, 55°C | Acetivibrio thermocellus | |
0.088 | - |
NADH | wild-type, pH 7.0, 55°C | Acetivibrio thermocellus | |
0.17 | - |
NADH | moderately ethanol producing strain, pH 7.0, 55°C | Acetivibrio thermocellus | |
1.4 | - |
acetaldehyde | cosubstrate NADH, moderately ethanol producing strain, pH 7.0, 55°C | Acetivibrio thermocellus | |
1.4 | - |
acetaldehyde | cosubstrate NADPH, moderately ethanol producing strain, pH 7.0, 55°C | Acetivibrio thermocellus | |
1.6 | - |
acetaldehyde | wild-type, pH 7.0, 55°C | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | A0A0H3W5U9 | bifunctional enzyme, C-terminal section belongs to the iron-containing alcohol dehydrogenase family, N-terminal section belongs to the aldehyde dehydrogenase family | - |
Thermoanaerobacterium saccharolyticum | A0A0H3W5V0 | bifunctional enzyme, C-terminal section belongs to the iron-containing alcohol dehydrogenase family, N-terminal section belongs to the aldehyde dehydrogenase family | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADH + H+ | - |
Acetivibrio thermocellus | ethanol + NAD+ | - |
? | |
acetaldehyde + NADH + H+ | - |
Thermoanaerobacterium saccharolyticum | ethanol + NAD+ | - |
? | |
acetaldehyde + NADPH + H+ | - |
Acetivibrio thermocellus | ethanol + NADP+ | - |
? | |
acetaldehyde + NADPH + H+ | - |
Thermoanaerobacterium saccharolyticum | ethanol + NADP+ | - |
? | |
additional information | in wild-type strains, NADH is the preferred cofactor for both aldehyde dehydrogenase ALDH and alcohol dehydrogenase ADH activities. In high-ethanol-producing (ethanologen) strains of Thermoanaerobacterium saccharolyticum, both ALDH and ADH activities show increased NADPH-linked activity | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
additional information | in wild-type strains, NADH is the preferred cofactor for both aldehyde dehydrogenase ALDH and alcohol dehydrogenase ADH activities. The AdhE protein of the ethanologenic strain of Clostridium thermocellum has acquired high NADPH-linked ADH activity while maintaining NADH-linked ALDH and ADH activities at wild-type levels | Acetivibrio thermocellus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
210 | - |
acetaldehyde | cosubstrate NADPH, moderately ethanol producing strain, pH 7.0, 55°C | Acetivibrio thermocellus | |
220 | - |
acetaldehyde | cosubstrate NADH, moderately ethanol producing strain, pH 7.0, 55°C | Acetivibrio thermocellus | |
220 | - |
NADH | moderately ethanol producing strain, pH 7.0, 55°C | Acetivibrio thermocellus | |
240 | - |
NADH | wild-type, pH 7.0, 55°C | Acetivibrio thermocellus | |
240 | - |
acetaldehyde | wild-type, pH 7.0, 55°C | Acetivibrio thermocellus | |
280 | - |
NADPH | moderately ethanol producing strain, pH 7.0, 55°C | Acetivibrio thermocellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | in wild-type strains, NADH is the preferred cofactor for both aldehyde dehydrogenase ALDH and alcohol dehydrogenase ADH activities. In high-ethanol-producing (ethanologen) strains of Thermoanaerobacterium saccharolyticum, both ALDH and ADH activities show increased NADPH-linked activity | Thermoanaerobacterium saccharolyticum | |
NADH | in wild-type strains, NADH is the preferred cofactor for both aldehyde dehydrogenase ALDH and alcohol dehydrogenase ADH activities. The AdhE protein of the ethanologenic strain of Clostridium thermocellum has acquired high NADPH-linked ADH activity while maintaining NADH-linked ALDH and ADH activities at wild-type levels | Acetivibrio thermocellus | |
NADPH | in wild-type strains, NADH is the preferred cofactor for both aldehyde dehydrogenase ALDH and alcohol dehydrogenase ADH activities. In high-ethanol-producing (ethanologen) strains of Thermoanaerobacterium saccharolyticum, both ALDH and ADH activities show increased NADPH-linked activity | Thermoanaerobacterium saccharolyticum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
150 | - |
acetaldehyde | cosubstrate NADH, moderately ethanol producing strain, pH 7.0, 55°C | Acetivibrio thermocellus | |
150 | - |
acetaldehyde | cosubstrate NADPH, moderately ethanol producing strain, pH 7.0, 55°C | Acetivibrio thermocellus | |
150 | - |
acetaldehyde | wild-type, pH 7.0, 55°C | Acetivibrio thermocellus | |
1300 | - |
NADH | moderately ethanol producing strain, pH 7.0, 55°C | Acetivibrio thermocellus | |
2700 | - |
NADH | wild-type, pH 7.0, 55°C | Acetivibrio thermocellus | |
3700 | - |
NADPH | moderately ethanol producing strain, pH 7.0, 55°C | Acetivibrio thermocellus |