BRENDA - Enzyme Database
show all sequences of 1.11.1.17

Characterization of glutathione amide reductase from Chromatium gracile. Identification of a novel thiol peroxidase (Prx/Grx) fueled by glutathione amide redox cycling

Vergauwen, B.; Pauwels, F.; Jacquemotte, F.; Meyer, T.E.; Cusanovich, M.A.; Bartsch, R.G.; van Beeumen, J.J.; J. Biol. Chem. 276, 20890-20897 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL(DE3)
Marichromatium gracile
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic analysis of the glutathione amide reductase, using glutathione amide disulfide (cf. EC 1.8.1.16) and NADH as substrates
Marichromatium gracile
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27430
-
calculated from the deduced amino acid sequence
Marichromatium gracile
27500
-
chimeric enzyme composed of one N-terminal peroxidoxin-like domain followed by a glutaredoxin-like C-terminus, SDS-PAGE
Marichromatium gracile
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Marichromatium gracile
-
-
-
Marichromatium gracile DSM 1712
-
-
-
Purification (Commentary)
Commentary
Organism
partial purification of the Prx/Grx-containing crude extract, centrifugation, ammonium sulfate precipitation, butyl-Sepharose HR 16/10 column, dialysis, ResourceQ column
Marichromatium gracile
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glutathione amide + H2O2
similar acitivity with small alkyl hydroperoxides indicating broad substrate specificity
698716
Marichromatium gracile
glutathione amide disulfide + H2O
-
-
-
?
glutathione amide + H2O2
similar acitivity with small alkyl hydroperoxides indicating broad substrate specificity
698716
Marichromatium gracile DSM 1712
glutathione amide disulfide + H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Marichromatium gracile
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic analysis of the glutathione amide reductase, using glutathione amide disulfide (cf. EC 1.8.1.16) and NADH as substrates
Marichromatium gracile
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.1
-
assay at
Marichromatium gracile
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Marichromatium gracile
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL(DE3)
Marichromatium gracile
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Marichromatium gracile
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic analysis of the glutathione amide reductase, using glutathione amide disulfide (cf. EC 1.8.1.16) and NADH as substrates
Marichromatium gracile
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27430
-
calculated from the deduced amino acid sequence
Marichromatium gracile
27500
-
chimeric enzyme composed of one N-terminal peroxidoxin-like domain followed by a glutaredoxin-like C-terminus, SDS-PAGE
Marichromatium gracile
Purification (Commentary) (protein specific)
Commentary
Organism
partial purification of the Prx/Grx-containing crude extract, centrifugation, ammonium sulfate precipitation, butyl-Sepharose HR 16/10 column, dialysis, ResourceQ column
Marichromatium gracile
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glutathione amide + H2O2
similar acitivity with small alkyl hydroperoxides indicating broad substrate specificity
698716
Marichromatium gracile
glutathione amide disulfide + H2O
-
-
-
?
glutathione amide + H2O2
similar acitivity with small alkyl hydroperoxides indicating broad substrate specificity
698716
Marichromatium gracile DSM 1712
glutathione amide disulfide + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Marichromatium gracile
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic analysis of the glutathione amide reductase, using glutathione amide disulfide (cf. EC 1.8.1.16) and NADH as substrates
Marichromatium gracile
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.1
-
assay at
Marichromatium gracile
General Information
General Information
Commentary
Organism
physiological function
garB encodes the central enzyme in glutathione amide cycling. The enzyme is the first example of a prokaryotic low molecular mass thiol-dependent peroxidase. The hydroperoxide-dependent Prx/Grx-catalyzed oxidation of glutathione amide is established in vitro, and as to be expected, the reaction is fueled by NADH via glutathione amide redox recycling
Marichromatium gracile
General Information (protein specific)
General Information
Commentary
Organism
physiological function
garB encodes the central enzyme in glutathione amide cycling. The enzyme is the first example of a prokaryotic low molecular mass thiol-dependent peroxidase. The hydroperoxide-dependent Prx/Grx-catalyzed oxidation of glutathione amide is established in vitro, and as to be expected, the reaction is fueled by NADH via glutathione amide redox recycling
Marichromatium gracile
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic analysis of the glutathione amide reductase, using glutathione amide disulfide (cf. EC 1.8.1.16) and NADH as substrates
Marichromatium gracile
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic analysis of the glutathione amide reductase, using glutathione amide disulfide (cf. EC 1.8.1.16) and NADH as substrates
Marichromatium gracile
Other publictions for EC 1.11.1.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
698716
Vergauwen
Characterization of glutathion ...
Marichromatium gracile, Marichromatium gracile DSM 1712
J. Biol. Chem.
276
20890-20897
2001
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1
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