Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 (DE3) cells | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
with 24% PEG 600, 0.2 M imidazole malate pH 5.5, 20 mM dithiothreitol | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | a single, tightly bound, Ca2+ ion at the domain interface of both the fully oxidized and mixed-valence forms of the enzyme is absolutely required for catalytic activity, reduction of the electron-transferring (high-potential) heme in the presence of Ca2+ ions triggers substantial structural rearrangements around the active-site (low-potential) heme to allow substrate binding and catalysis | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P14532 | - |
- |
Purification (Comment) | Organism |
---|---|
S-Sepharose column chromatography and Superdex-75 gel filtration | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + H2O2 | - |
Pseudomonas aeruginosa | ferricytochrome c + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CCP | - |
Pseudomonas aeruginosa |
cytochrome c peroxidase | - |
Pseudomonas aeruginosa |