Literature summary for 1.14.11.21 extracted from

Shrestha, B.; Dhakal, D.; Darsandhari, S.; Pandey, R.; Pokhrel, A.; Jnawali, H.; Sohng, J.
Heterologous production of clavulanic acid intermediates in Streptomyces venezuelae (2017), Biotechnol. Bioprocess Eng., 22, 359-365 .

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Application

Application	Comment	Organism
synthesis	clavulanic acid intermediates: deoxygaunidinoproclavaminic acid, guanidinoproclavaminic acid, and dihydroclavaminic acid are heterologously produced in Streptomyces venezuelae recombinant using four sets of early genes from the clavulanic acid biosynthetic pathway	Streptomyces clavuligerus

Cloned(Commentary)

Cloned (Comment)	Organism
Streptomyces venezuelae YJ028 is utilized as the heterologous host for the expression of four structural clavulanic acid biosynthesis genes, which encode carboxyethylarginine synthase (ceas2), beta-lactam synthetase (bls2), clavaminate synthase (cas2), and proclavaminate amidinohydrolase (pah2). The genes are cloned into pIBR25 expression vector containing ermE promoter to generate pBS4. The cas2 gene is also cloned into pSET152 to generate pCas2. It is then integrated into the genome of Streptomyces venezuelae YJ028	Streptomyces clavuligerus

Cloned (Comment)

Organism

Streptomyces venezuelae YJ028 is utilized as the heterologous host for the expression of four structural clavulanic acid biosynthesis genes, which encode carboxyethylarginine synthase (ceas2), beta-lactam synthetase (bls2), clavaminate synthase (cas2), and proclavaminate amidinohydrolase (pah2). The genes are cloned into pIBR25 expression vector containing ermE promoter to generate pBS4. The cas2 gene is also cloned into pSET152 to generate pCas2. It is then integrated into the genome of Streptomyces venezuelae YJ028

Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(3S,5S)-dihydroclavaminate + 2-oxoglutarate + O2	Streptomyces clavuligerus	the enzyme is involved in clavulanic acid biosynthesis	(3S,5S)-clavaminate + succinate + CO2 + H2O	-	?
proclavaminate + 2-oxoglutarate + O2	Streptomyces clavuligerus	the enzyme is involved in clavulanic acid biosynthesis	dihydroclavaminate + succinate + CO2 + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces clavuligerus	Q53939	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3S,5S)-dihydroclavaminate + 2-oxoglutarate + O2	the enzyme is involved in clavulanic acid biosynthesis	Streptomyces clavuligerus	(3S,5S)-clavaminate + succinate + CO2 + H2O	-	?
proclavaminate + 2-oxoglutarate + O2	the enzyme is involved in clavulanic acid biosynthesis	Streptomyces clavuligerus	dihydroclavaminate + succinate + CO2 + H2O	-	?

Synonyms

Synonyms	Comment	Organism
CAS2	-	Streptomyces clavuligerus

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in clavulanic acid biosynthesis	Streptomyces clavuligerus