BRENDA - Enzyme Database show
show all sequences of 1.14.14.141

Isolation and functional characterization of CYP71AJ4 encoding for the first P450 monooxygenase of angular furanocoumarin biosynthesis

Larbat, R.; Hehn, A.; Hans, J.; Schneider, S.; Jugde, H.; Schneider, B.; Matern, U.; Bourgaud, F.; J. Biol. Chem. 284, 4776-4785 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in yeast strain WAT 11
Apium graveolens
expressed in yeast strain WAT 11
Pastinaca sativa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00054
-
(+)-marmesin
-
Apium graveolens
0.0013
-
(+)-marmesin
-
Pastinaca sativa
0.019
-
5-hydroxymarmesin
-
Pastinaca sativa
0.054
-
5-hydroxymarmesin
-
Apium graveolens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-hydroxymarmesin + [reduced NADPH-hemoprotein reductase] + O2
Apium graveolens
-
bergaptol + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
5-hydroxymarmesin + [reduced NADPH-hemoprotein reductase] + O2
Pastinaca sativa
-
bergaptol + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Apium graveolens
C0SJS4
-
-
Pastinaca sativa
C0SJS2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2
-
698971
Apium graveolens
psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
-
-
?
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2
-
698971
Pastinaca sativa
psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
-
-
?
5-hydroxymarmesin + [reduced NADPH-hemoprotein reductase] + O2
-
698971
Apium graveolens
bergaptol + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-hydroxymarmesin + [reduced NADPH-hemoprotein reductase] + O2
-
698971
Pastinaca sativa
bergaptol + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in yeast strain WAT 11
Apium graveolens
expressed in yeast strain WAT 11
Pastinaca sativa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00054
-
(+)-marmesin
-
Apium graveolens
0.0013
-
(+)-marmesin
-
Pastinaca sativa
0.019
-
5-hydroxymarmesin
-
Pastinaca sativa
0.054
-
5-hydroxymarmesin
-
Apium graveolens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-hydroxymarmesin + [reduced NADPH-hemoprotein reductase] + O2
Apium graveolens
-
bergaptol + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
5-hydroxymarmesin + [reduced NADPH-hemoprotein reductase] + O2
Pastinaca sativa
-
bergaptol + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2
-
698971
Apium graveolens
psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
-
-
?
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2
-
698971
Pastinaca sativa
psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
-
-
?
5-hydroxymarmesin + [reduced NADPH-hemoprotein reductase] + O2
-
698971
Apium graveolens
bergaptol + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-hydroxymarmesin + [reduced NADPH-hemoprotein reductase] + O2
-
698971
Pastinaca sativa
bergaptol + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.141
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728497
Parast
In vitro isolation, elicitatio ...
Cullen corylifolium
Plant Physiol. Biochem.
49
1138-1146
2011
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698971
Larbat
Isolation and functional chara ...
Apium graveolens, Pastinaca sativa
J. Biol. Chem.
284
4776-4785
2009
-
-
2
-
-
-
-
4
-
-
-
2
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
4
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674887
Larbat
Molecular cloning and function ...
Ammi majus
J. Biol. Chem.
282
542-554
2007
1
-
1
-
1
-
2
1
1
1
1
2
-
6
-
-
1
1
-
2
-
-
4
2
1
-
-
1
1
-
-
1
-
1
-
1
-
1
1
-
1
-
-
2
-
1
1
1
1
2
-
-
-
1
-
2
-
-
4
2
1
-
-
1
1
-
-
1
-
-
-
-
-
-
673426
Hamerski
Elicitor-induced biosynthesis ...
Ammi majus
Eur. J. Biochem.
171
369-375
1988
1
-
-
-
-
-
6
-
2
-
-
2
-
4
-
-
1
-
-
1
-
-
4
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
6
-
-
2
-
-
2
-
-
-
1
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
673425
Wendorff
Differential response of cultu ...
Petroselinum crispum
Eur. J. Biochem.
161
391-398
1986
2
-
-
-
-
-
10
1
2
1
-
1
-
5
-
-
1
1
-
1
-
-
3
-
1
-
1
-
1
-
-
1
-
-
-
2
-
-
1
-
-
-
-
10
-
1
2
1
-
1
-
-
-
1
-
1
-
-
3
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-