BRENDA - Enzyme Database show
show all sequences of 1.14.14.161

7-deoxyloganetic acid synthase catalyzes a key 3 step oxidation to form 7-deoxyloganetic acid in Catharanthus roseus iridoid biosynthesis

Salim, V.; Wiens, B.; Masada-Atsumi, S.; Yu, F.; De Luca, V.; Phytochemistry 101, 23-31 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of the gene in Saccharomyces cerevisiae confirms its function as an authentic 7-deoxyloganetic acid synthase that catalyzes the 3 step oxidation of iridodial-nepetalactol to form 7-deoxyloganetic acid
Catharanthus roseus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-cis,trans-nepetalactol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
Catharanthus roseus
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids, overall reaction
7-deoxyloganetate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
?
(+)-cis,trans-nepetalactol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
Catharanthus roseus cv. Little Delicata
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids, overall reaction
7-deoxyloganetate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
W8JIS5
-
-
Catharanthus roseus cv. Little Delicata
W8JIS5
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-cis,trans-nepetalactol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids, overall reaction
748875
Catharanthus roseus
7-deoxyloganetate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
(+)-cis,trans-nepetalactol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids, overall reaction
748875
Catharanthus roseus cv. Little Delicata
7-deoxyloganetate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
(+)-cis,trans-nepetalactol + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids
748875
Catharanthus roseus
7-deoxyloganetic alcohol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(+)-cis,trans-nepetalactol + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids
748875
Catharanthus roseus cv. Little Delicata
7-deoxyloganetic alcohol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
7-deoxyloganetic alcohol + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids
748875
Catharanthus roseus
iridotrial + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
7-deoxyloganetic alcohol + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids
748875
Catharanthus roseus cv. Little Delicata
iridotrial + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
iridotrial + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids
748875
Catharanthus roseus
7-deoxyloganetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
iridotrial + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids
748875
Catharanthus roseus cv. Little Delicata
7-deoxyloganetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
a cytochrome P-450 (heme thiolate) enzyme
Catharanthus roseus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of the gene in Saccharomyces cerevisiae confirms its function as an authentic 7-deoxyloganetic acid synthase that catalyzes the 3 step oxidation of iridodial-nepetalactol to form 7-deoxyloganetic acid
Catharanthus roseus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
a cytochrome P-450 (heme thiolate) enzyme
Catharanthus roseus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-cis,trans-nepetalactol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
Catharanthus roseus
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids, overall reaction
7-deoxyloganetate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
?
(+)-cis,trans-nepetalactol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
Catharanthus roseus cv. Little Delicata
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids, overall reaction
7-deoxyloganetate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-cis,trans-nepetalactol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids, overall reaction
748875
Catharanthus roseus
7-deoxyloganetate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
(+)-cis,trans-nepetalactol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids, overall reaction
748875
Catharanthus roseus cv. Little Delicata
7-deoxyloganetate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
(+)-cis,trans-nepetalactol + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids
748875
Catharanthus roseus
7-deoxyloganetic alcohol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(+)-cis,trans-nepetalactol + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids
748875
Catharanthus roseus cv. Little Delicata
7-deoxyloganetic alcohol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
7-deoxyloganetic alcohol + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids
748875
Catharanthus roseus
iridotrial + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
7-deoxyloganetic alcohol + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids
748875
Catharanthus roseus cv. Little Delicata
iridotrial + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
iridotrial + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids
748875
Catharanthus roseus
7-deoxyloganetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
iridotrial + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids
748875
Catharanthus roseus cv. Little Delicata
7-deoxyloganetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
virus-induced gene silencing of 7-deoxyloganetic acid synthase in Catharanthus roseus greatly decreases levels of secologanin and the major monoterpenoid indole alkaloids, catharanthine and vindoline in silenced leaves
Catharanthus roseus
metabolism
the enzyme catalyses three successive reactions in the seco-iridoid pathway leading to biosynthesis of monoterpenoid indole alkaloids
Catharanthus roseus
General Information (protein specific)
General Information
Commentary
Organism
malfunction
virus-induced gene silencing of 7-deoxyloganetic acid synthase in Catharanthus roseus greatly decreases levels of secologanin and the major monoterpenoid indole alkaloids, catharanthine and vindoline in silenced leaves
Catharanthus roseus
metabolism
the enzyme catalyses three successive reactions in the seco-iridoid pathway leading to biosynthesis of monoterpenoid indole alkaloids
Catharanthus roseus
Other publictions for EC 1.14.14.161
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743934
Campbell
Engineering of a nepetalactol ...
Catharanthus roseus
ACS Synth. Biol.
5
405-414
2016
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1
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748736
Miettinen
The seco-iridoid pathway from ...
Catharanthus roseus
Nat. Commun.
5
3606
2014
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4
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1
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1
1
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1
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1
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2
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4
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1
1
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748875
Salim
7-deoxyloganetic acid synthas ...
Catharanthus roseus, Catharanthus roseus cv. Little Delicata
Phytochemistry
101
23-31
2014
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8
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2
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