Literature summary for 1.14.14.86 extracted from

Villa-Ruano, N.; Lozoya-Gloria, E.; Betancourt-Jimenez, M.; Herlt, T.; Pacheco-Hernandez, Y.; Castro-Juarez, C.
Functional characterization of ent-kaurene oxidase, MtKO, from Montanoa tomentosa (zoapatle) (2015), Arch. Biol. Sci., 67, 193-199 .

No PubMed abstract available

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Cloned(Commentary)

Cloned (Comment)	Organism
cloned from leaf microsomes, functional recombinant expression in Saccharomyces cerevisiae	Montanoa tomentosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.08032	-	ent-kaur-16-ene	pH 7.2, 30°C, leaf microsomes	Montanoa tomentosa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Montanoa tomentosa	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2	Montanoa tomentosa	overall reaction	ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Montanoa tomentosa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme partially as leaves microsomes	Montanoa tomentosa

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Montanoa tomentosa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2	overall reaction	Montanoa tomentosa	ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O	-	?

Synonyms

Synonyms	Comment	Organism
MtKO	-	Montanoa tomentosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Montanoa tomentosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Montanoa tomentosa

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Montanoa tomentosa
FAD	-	Montanoa tomentosa
NADPH	-	Montanoa tomentosa

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Release 2023.1 (January 2023)