BRENDA - Enzyme Database show
show all sequences of 1.14.14.89

CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase

Akashi, T.; Aoki, T.; Ayabe, S.I.; Biochem. Biophys. Res. Commun. 251, 67-70 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisae overexpressing CYP81E1
Glycyrrhiza echinata
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Glycyrrhiza echinata
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formononetin + [reduced NADPH-hemoprotein reductase] + O2
Glycyrrhiza echinata
cytochrome P450-dependent monooxygenase
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycyrrhiza echinata
-
elicitor-induced
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Glycyrrhiza echinata
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
daidzein + [reduced NADPH-hemoprotein reductase] + O2
-
285323
Glycyrrhiza echinata
2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
-
285323
Glycyrrhiza echinata
ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2
-
285323
Glycyrrhiza echinata
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2
cytochrome P450-dependent monooxygenase
285323
Glycyrrhiza echinata
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
genistein + [reduced NADPH-hemoprotein reductase] + O2
-
285323
Glycyrrhiza echinata
2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
-
285323
Glycyrrhiza echinata
ir
additional information
no substrates are 5,7,4'-trihydroxyflavone, 7,4'-dihydroxyflavone, trans-cinnamic acid and 4-coumaric acid
285323
Glycyrrhiza echinata
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisae overexpressing CYP81E1
Glycyrrhiza echinata
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Glycyrrhiza echinata
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formononetin + [reduced NADPH-hemoprotein reductase] + O2
Glycyrrhiza echinata
cytochrome P450-dependent monooxygenase
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
ir
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Glycyrrhiza echinata
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
daidzein + [reduced NADPH-hemoprotein reductase] + O2
-
285323
Glycyrrhiza echinata
2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
-
285323
Glycyrrhiza echinata
ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2
-
285323
Glycyrrhiza echinata
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2
cytochrome P450-dependent monooxygenase
285323
Glycyrrhiza echinata
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
ir
genistein + [reduced NADPH-hemoprotein reductase] + O2
-
285323
Glycyrrhiza echinata
2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
-
285323
Glycyrrhiza echinata
ir
additional information
no substrates are 5,7,4'-trihydroxyflavone, 7,4'-dihydroxyflavone, trans-cinnamic acid and 4-coumaric acid
285323
Glycyrrhiza echinata
?
-
-
-
-
Other publictions for EC 1.14.14.89
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745660
Gao
Reduction of soy isoflavones ...
Slackia sp. AUH-JLC159
Lett. Appl. Microbiol.
63
111-116
2016
-
-
1
-
1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285324
Shimada
Induction of isoflavonoid path ...
Lotus japonicus
Plant Sci.
160
37-47
2000
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285323
Akashi
CYP81E1, a cytochrome P450 cDN ...
Glycyrrhiza echinata
Biochem. Biophys. Res. Commun.
251
67-70
1998
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285320
Clemens
-
Characterization of cytochrome ...
Cicer arietinum
Phytochemistry
32
653-657
1993
-
-
-
-
-
-
7
2
-
1
-
2
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
1
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285319
Daniel
Elicitor-induced metabolic cha ...
Cicer arietinum
Planta
182
270-278
1990
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285318
Hinderer
-
Microsomal isoflavone 2'- and ...
Cicer arietinum
FEBS Lett.
214
101-106
1987
2
-
-
-
-
-
2
-
1
-
-
2
-
1
-
-
-
-
-
2
-
1
9
-
-
-
-
-
1
-
-
1
-
-
-
2
-
-
1
-
-
-
-
2
-
-
1
-
-
2
-
-
-
-
-
2
-
1
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285325
Kochs
Enzymic synthesis of isoflavon ...
Glycine max
Eur. J. Biochem.
155
311-318
1986
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-