BRENDA - Enzyme Database show
show all sequences of 1.14.14.89

Reduction of soy isoflavones by use of Escherichia coli whole-cell biocatalyst expressing isoflavone reductase under aerobic conditions

Gao, Y.N.; Hao, Q.H.; Zhang, H.L.; Zhou, B.; Yu, X.M.; Wang, X.L.; Lett. Appl. Microbiol. 63, 111-116 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene dgr, recombinant enzyme expression in Escherichia coli strain BL21(DE3), aerobic bioconversion capacity of the recombinant Escherichia coli whole-cell under the optimized conditions, method evaluation and optimization, overview
Slackia sp. AUH-JLC159
Engineering
Amino acid exchange
Commentary
Organism
additional information
reduction of soy isoflavones by use of Escherichia coli whole-cell biocatalyst expressing isoflavone reductase under aerobic conditions, optimization of the aerobic bioconversion conditions, overview. Addition of chloramphenicol significantly increases the bioconversion capacity of the recombinant Escherichia coli whole-cell, but addition of chloramphenicol does not increase the expression of S-DGR irrespective of whether the concentration of chloramphenicol is high. The maximal concentration of daidzein that the recombinant Escherichia coli whole-cell is able to convert efficiently is 0.8 mmol/l. The average bioconversion rate for daidzein is 85.0% and the yield of 2'-hydroxydaidzein is 0.545 mmol/l
Slackia sp. AUH-JLC159
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
daidzein + [reduced NADPH-hemoprotein reductase] + O2
Slackia sp. AUH-JLC159
-
2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
genistein + [reduced NADPH-hemoprotein reductase] + O2
Slackia sp. AUH-JLC159
-
2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Slackia sp. AUH-JLC159
A0A0U1WKA6
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
daidzein + [reduced NADPH-hemoprotein reductase] + O2
-
745660
Slackia sp. AUH-JLC159
2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
genistein + [reduced NADPH-hemoprotein reductase] + O2
-
745660
Slackia sp. AUH-JLC159
2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
reduction of soy isoflavones daidzein and genistein under aerobic conditions by use of the recombinant Escherichia coli whole-cell expressing isoflavone reductase
745660
Slackia sp. AUH-JLC159
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Slackia sp. AUH-JLC159
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Slackia sp. AUH-JLC159
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
-
Slackia sp. AUH-JLC159
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Slackia sp. AUH-JLC159
Cloned(Commentary) (protein specific)
Commentary
Organism
gene dgr, recombinant enzyme expression in Escherichia coli strain BL21(DE3), aerobic bioconversion capacity of the recombinant Escherichia coli whole-cell under the optimized conditions, method evaluation and optimization, overview
Slackia sp. AUH-JLC159
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
-
Slackia sp. AUH-JLC159
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Slackia sp. AUH-JLC159
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
reduction of soy isoflavones by use of Escherichia coli whole-cell biocatalyst expressing isoflavone reductase under aerobic conditions, optimization of the aerobic bioconversion conditions, overview. Addition of chloramphenicol significantly increases the bioconversion capacity of the recombinant Escherichia coli whole-cell, but addition of chloramphenicol does not increase the expression of S-DGR irrespective of whether the concentration of chloramphenicol is high. The maximal concentration of daidzein that the recombinant Escherichia coli whole-cell is able to convert efficiently is 0.8 mmol/l. The average bioconversion rate for daidzein is 85.0% and the yield of 2'-hydroxydaidzein is 0.545 mmol/l
Slackia sp. AUH-JLC159
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
daidzein + [reduced NADPH-hemoprotein reductase] + O2
Slackia sp. AUH-JLC159
-
2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
genistein + [reduced NADPH-hemoprotein reductase] + O2
Slackia sp. AUH-JLC159
-
2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
daidzein + [reduced NADPH-hemoprotein reductase] + O2
-
745660
Slackia sp. AUH-JLC159
2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
genistein + [reduced NADPH-hemoprotein reductase] + O2
-
745660
Slackia sp. AUH-JLC159
2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
reduction of soy isoflavones daidzein and genistein under aerobic conditions by use of the recombinant Escherichia coli whole-cell expressing isoflavone reductase
745660
Slackia sp. AUH-JLC159
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Slackia sp. AUH-JLC159
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Slackia sp. AUH-JLC159
Other publictions for EC 1.14.14.89
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745660
Gao
Reduction of soy isoflavones ...
Slackia sp. AUH-JLC159
Lett. Appl. Microbiol.
63
111-116
2016
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1
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1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285324
Shimada
Induction of isoflavonoid path ...
Lotus japonicus
Plant Sci.
160
37-47
2000
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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1
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-
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-
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285323
Akashi
CYP81E1, a cytochrome P450 cDN ...
Glycyrrhiza echinata
Biochem. Biophys. Res. Commun.
251
67-70
1998
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
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1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285320
Clemens
-
Characterization of cytochrome ...
Cicer arietinum
Phytochemistry
32
653-657
1993
-
-
-
-
-
-
7
2
-
1
-
2
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
1
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285319
Daniel
Elicitor-induced metabolic cha ...
Cicer arietinum
Planta
182
270-278
1990
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
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-
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-
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1
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1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285318
Hinderer
-
Microsomal isoflavone 2'- and ...
Cicer arietinum
FEBS Lett.
214
101-106
1987
2
-
-
-
-
-
2
-
1
-
-
2
-
1
-
-
-
-
-
2
-
1
9
-
-
-
-
-
1
-
-
1
-
-
-
2
-
-
1
-
-
-
-
2
-
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1
-
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2
-
-
-
-
-
2
-
1
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285325
Kochs
Enzymic synthesis of isoflavon ...
Glycine max
Eur. J. Biochem.
155
311-318
1986
1
-
-
-
-
-
-
-
1
-
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1
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1
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1
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1
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1
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1
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1
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