Cloned (Comment) | Organism |
---|---|
gene dgr, recombinant enzyme expression in Escherichia coli strain BL21(DE3), aerobic bioconversion capacity of the recombinant Escherichia coli whole-cell under the optimized conditions, method evaluation and optimization, overview | Slackia sp. AUH-JLC159 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | reduction of soy isoflavones by use of Escherichia coli whole-cell biocatalyst expressing isoflavone reductase under aerobic conditions, optimization of the aerobic bioconversion conditions, overview. Addition of chloramphenicol significantly increases the bioconversion capacity of the recombinant Escherichia coli whole-cell, but addition of chloramphenicol does not increase the expression of S-DGR irrespective of whether the concentration of chloramphenicol is high. The maximal concentration of daidzein that the recombinant Escherichia coli whole-cell is able to convert efficiently is 0.8 mmol/l. The average bioconversion rate for daidzein is 85.0% and the yield of 2'-hydroxydaidzein is 0.545 mmol/l | Slackia sp. AUH-JLC159 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
daidzein + [reduced NADPH-hemoprotein reductase] + O2 | Slackia sp. AUH-JLC159 | - |
2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
genistein + [reduced NADPH-hemoprotein reductase] + O2 | Slackia sp. AUH-JLC159 | - |
2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Slackia sp. AUH-JLC159 | A0A0U1WKA6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
daidzein + [reduced NADPH-hemoprotein reductase] + O2 | - |
Slackia sp. AUH-JLC159 | 2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
genistein + [reduced NADPH-hemoprotein reductase] + O2 | - |
Slackia sp. AUH-JLC159 | 2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | reduction of soy isoflavones daidzein and genistein under aerobic conditions by use of the recombinant Escherichia coli whole-cell expressing isoflavone reductase | Slackia sp. AUH-JLC159 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
daidzein and genistein reductase | - |
Slackia sp. AUH-JLC159 |
dgr | - |
Slackia sp. AUH-JLC159 |
S-DGR | - |
Slackia sp. AUH-JLC159 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Slackia sp. AUH-JLC159 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Slackia sp. AUH-JLC159 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | - |
Slackia sp. AUH-JLC159 | |
NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Slackia sp. AUH-JLC159 |