BRENDA - Enzyme Database show
show all sequences of 1.14.19.67

CYP719B1 is salutaridine synthase, the C-C phenol-coupling enzyme of morphine biosynthesis in opium poppy

Gesell, A.; Rolf, M.; Ziegler, J.; Diaz Chavez, M.L.; Huang, F.C.; Kutchan, T.M.; J. Biol. Chem. 284, 24432-24442 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
full-length CYP719B1 cDNA is generated by amplification out of bacterial vector pCR2.1 and inserted into baculovirus transfer vector pVL1392
Papaver somniferum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0062
-
(R)-reticuline
-
Papaver somniferum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
determined by SDS-PAGE
Papaver somniferum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
Papaver somniferum
-
salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Papaver somniferum
B1NF18
-
-
Purification (Commentary)
Commentary
Organism
microsomes from insect cells are prepared
Papaver somniferum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-norreticuline + [reduced NADPH-hemoprotein reductase] + O2
-
698930
Papaver somniferum
norsalutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
-
698930
Papaver somniferum
salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay for kinetic analysis
Papaver somniferum
37
-
activity assay
Papaver somniferum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.027
-
(R)-reticuline
-
Papaver somniferum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
activity assay
Papaver somniferum
8
-
assay for kinetic analysis
Papaver somniferum
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Papaver somniferum
Cloned(Commentary) (protein specific)
Commentary
Organism
full-length CYP719B1 cDNA is generated by amplification out of bacterial vector pCR2.1 and inserted into baculovirus transfer vector pVL1392
Papaver somniferum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Papaver somniferum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0062
-
(R)-reticuline
-
Papaver somniferum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
determined by SDS-PAGE
Papaver somniferum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
Papaver somniferum
-
salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
microsomes from insect cells are prepared
Papaver somniferum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-norreticuline + [reduced NADPH-hemoprotein reductase] + O2
-
698930
Papaver somniferum
norsalutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
-
698930
Papaver somniferum
salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay for kinetic analysis
Papaver somniferum
37
-
activity assay
Papaver somniferum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.027
-
(R)-reticuline
-
Papaver somniferum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
activity assay
Papaver somniferum
8
-
assay for kinetic analysis
Papaver somniferum
Other publictions for EC 1.14.19.67
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743574
Fossati
Synthesis of morphinan alkalo ...
Papaver somniferum
PLoS ONE
10
e0124459
2015
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1
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-
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3
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1
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1
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1
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728475
Onoyovwe
Morphine biosynthesis in opium ...
Papaver somniferum
Plant Cell
25
4110-4122
2013
-
-
-
-
-
-
-
-
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1
1
-
2
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5
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1
1
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-
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1
1
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5
-
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1
1
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-
-
-
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-
-
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-
720707
Wijekoon
Systematic knockdown of morphi ...
Papaver somniferum
Plant J.
69
1052-1063
2012
-
-
-
-
-
-
-
-
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1
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2
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2
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1
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2
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-
-
-
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-
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1
1
-
-
-
698930
Gesell
CYP719B1 is salutaridine synth ...
Papaver somniferum
J. Biol. Chem.
284
24432-24442
2009
-
-
1
-
-
-
-
1
-
-
1
1
-
4
-
-
1
-
-
-
-
-
2
-
2
-
-
1
2
-
-
1
-
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1
1
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1
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1
1
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1
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2
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2
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1
2
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393876
Amann
-
Purification and characterizat ...
Bos taurus, Homo sapiens, Mus musculus, Ovis aries, Rattus norvegicus, Sus scrofa
Heterocycles
1
425-440
1995
-
-
-
-
-
-
-
1
9
-
1
1
-
6
-
-
1
-
-
1
-
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3
1
-
-
-
-
-
-
-
7
-
-
-
-
-
-
7
-
-
-
-
-
-
1
9
-
1
1
-
-
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1
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1
-
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3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393875
Gerardy R.; Zenk
-
Formation of salutaridine from ...
Papaver somniferum
Phytochemistry
32
79-86
1993
-
-
-
-
-
-
3
2
2
-
-
1
-
1
-
-
-
1
-
5
-
4
2
-
1
1
-
-
1
1
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1
-
-
-
-
-
-
1
-
-
-
-
3
-
2
2
-
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1
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5
-
4
2
-
1
1
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-
1
1
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