BRENDA - Enzyme Database
show all sequences of 1.2.1.4

NADP+-dependent aldehyde dehydrogenase from Acetobacter rancens CCM 1774: purification and properties

Hommel, R.; Kurth, J.; Kleber, H.P.; J. Basic Microbiol. 28, 25-33 (1988)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
-
Acetobacter pasteurianus
EDTA
0.5 mM, 50% inhibition
Acetobacter pasteurianus
ethyl acetate
-
Acetobacter pasteurianus
Fe2+
1 mM ZnCl2, complete inhibition, irreversible
Acetobacter pasteurianus
Hg2+
0.5 mM HgCl2, complete inhibition, irreversible
Acetobacter pasteurianus
NADPH
product inhibition
Acetobacter pasteurianus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.019
-
NADP+
in presence of propanal
Acetobacter pasteurianus
0.031
-
acetaldehyde
-
Acetobacter pasteurianus
0.15
-
propanal
-
Acetobacter pasteurianus
0.38
-
formaldehyde
-
Acetobacter pasteurianus
0.98
-
Butanal
-
Acetobacter pasteurianus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Acetobacter pasteurianus
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
82000
-
gel filtration
Acetobacter pasteurianus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Acetobacter pasteurianus
the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids
?
-
-
?
additional information
Acetobacter pasteurianus CCM 1774
the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Acetobacter pasteurianus
-
CCM 1774
-
Acetobacter pasteurianus CCM 1774
-
CCM 1774
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Acetobacter pasteurianus
Reaction
Reaction
Commentary
Organism
Reaction ID
an aldehyde + NADP+ + H2O = a carboxylate + NADPH + H+
random kinetic mechanism
Acetobacter pasteurianus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Acetobacter pasteurianus
Storage Stability
Storage Stability
Organism
4°C, phosphate buffer, pH 6.8-7.5, half-life: 5 months
Acetobacter pasteurianus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetaldehyde + NADP+ + H2O
-
437829
Acetobacter pasteurianus
acetate + NADPH + H+
-
-
-
?
acetaldehyde + NADP+ + H2O
-
437829
Acetobacter pasteurianus CCM 1774
acetate + NADPH + H+
-
-
-
?
butanal + NADP+ + H2O
-
437829
Acetobacter pasteurianus
butyrate + NADPH + H+
-
-
-
?
butanal + NADP+ + H2O
-
437829
Acetobacter pasteurianus CCM 1774
butyrate + NADPH + H+
-
-
-
?
formaldehyde + NADP+ + H2O
low activity
437829
Acetobacter pasteurianus
formate + NADPH + H+
-
-
-
?
formaldehyde + NADP+ + H2O
low activity
437829
Acetobacter pasteurianus CCM 1774
formate + NADPH + H+
-
-
-
?
additional information
the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids
437829
Acetobacter pasteurianus
?
-
-
-
?
additional information
the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids
437829
Acetobacter pasteurianus CCM 1774
?
-
-
-
?
propanal + NADP+ + H2O
-
437829
Acetobacter pasteurianus
propionate + NADPH + H+
-
-
-
?
propanal + NADP+ + H2O
-
437829
Acetobacter pasteurianus CCM 1774
propionate + NADPH + H+
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Acetobacter pasteurianus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
9.2
pH 5.0: 10% of maximal activity, pH 9.2: 15% of maximal activity
Acetobacter pasteurianus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
9.5
30 min, stable
Acetobacter pasteurianus
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Acetobacter pasteurianus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.043
-
NADPH
-
Acetobacter pasteurianus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Acetobacter pasteurianus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
-
Acetobacter pasteurianus
EDTA
0.5 mM, 50% inhibition
Acetobacter pasteurianus
ethyl acetate
-
Acetobacter pasteurianus
Fe2+
1 mM ZnCl2, complete inhibition, irreversible
Acetobacter pasteurianus
Hg2+
0.5 mM HgCl2, complete inhibition, irreversible
Acetobacter pasteurianus
NADPH
product inhibition
Acetobacter pasteurianus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.043
-
NADPH
-
Acetobacter pasteurianus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.019
-
NADP+
in presence of propanal
Acetobacter pasteurianus
0.031
-
acetaldehyde
-
Acetobacter pasteurianus
0.15
-
propanal
-
Acetobacter pasteurianus
0.38
-
formaldehyde
-
Acetobacter pasteurianus
0.98
-
Butanal
-
Acetobacter pasteurianus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Acetobacter pasteurianus
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
82000
-
gel filtration
Acetobacter pasteurianus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Acetobacter pasteurianus
the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids
?
-
-
?
additional information
Acetobacter pasteurianus CCM 1774
the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Acetobacter pasteurianus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Acetobacter pasteurianus
Storage Stability (protein specific)
Storage Stability
Organism
4°C, phosphate buffer, pH 6.8-7.5, half-life: 5 months
Acetobacter pasteurianus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetaldehyde + NADP+ + H2O
-
437829
Acetobacter pasteurianus
acetate + NADPH + H+
-
-
-
?
acetaldehyde + NADP+ + H2O
-
437829
Acetobacter pasteurianus CCM 1774
acetate + NADPH + H+
-
-
-
?
butanal + NADP+ + H2O
-
437829
Acetobacter pasteurianus
butyrate + NADPH + H+
-
-
-
?
butanal + NADP+ + H2O
-
437829
Acetobacter pasteurianus CCM 1774
butyrate + NADPH + H+
-
-
-
?
formaldehyde + NADP+ + H2O
low activity
437829
Acetobacter pasteurianus
formate + NADPH + H+
-
-
-
?
formaldehyde + NADP+ + H2O
low activity
437829
Acetobacter pasteurianus CCM 1774
formate + NADPH + H+
-
-
-
?
additional information
the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids
437829
Acetobacter pasteurianus
?
-
-
-
?
additional information
the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids
437829
Acetobacter pasteurianus CCM 1774
?
-
-
-
?
propanal + NADP+ + H2O
-
437829
Acetobacter pasteurianus
propionate + NADPH + H+
-
-
-
?
propanal + NADP+ + H2O
-
437829
Acetobacter pasteurianus CCM 1774
propionate + NADPH + H+
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Acetobacter pasteurianus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
9.2
pH 5.0: 10% of maximal activity, pH 9.2: 15% of maximal activity
Acetobacter pasteurianus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
9.5
30 min, stable
Acetobacter pasteurianus
Other publictions for EC 1.2.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739966
Bezsudnova
NADP-dependent aldehyde dehydr ...
Pyrobaculum ferrireducens, Pyrobaculum ferrireducens 1860
Archaea
2016
9127857
2016
-
-
1
1
-
-
-
3
-
-
-
-
-
4
-
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1
-
-
-
-
-
8
-
2
1
1
-
3
-
1
-
1
-
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1
1
1
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3
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1
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8
-
1
1
-
3
-
1
-
-
-
-
-
-
3
3
723749
Mihasan
Evidence of a plasmid-encoded ...
Paenarthrobacter nicotinovorans
Res. Microbiol.
164
22-30
2013
-
-
1
-
-
-
-
9
-
-
-
-
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1
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8
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1
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9
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2
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1
2
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9
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8
-
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9
-
-
-
-
-
-
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-
9
9
724459
Mukhopadhyay
Mitochondrial NAD dependent al ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1830
3391-3398
2013
-
-
1
-
2
-
-
-
5
-
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1
-
5
-
-
1
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6
-
1
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2
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1
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1
1
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2
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5
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1
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1
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712174
Alka
Substrate profiling and aldehy ...
Rattus norvegicus
Int. J. Biochem. Cell Biol.
42
2012-2018
2010
-
-
1
-
-
-
-
-
-
-
-
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-
1
-
-
1
-
-
1
-
-
17
-
1
-
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-
1
-
-
-
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-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
17
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688781
Patel
ALDH1A1 and ALDH3A1 expression ...
Homo sapiens
Lung Cancer
59
340-349
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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5
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1
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1
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1
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1
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5
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1
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-
-
-
-
673477
Estey
ALDH3A1: a corneal crystallin ...
Oryctolagus cuniculus, Homo sapiens, Mammalia, Mus musculus
Exp. Eye Res.
84
3-12
2007
-
4
-
-
-
-
4
20
4
-
-
4
-
4
-
-
-
-
-
5
-
-
20
-
12
-
-
-
-
-
-
-
4
-
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-
4
-
4
-
-
-
-
4
-
20
4
-
-
4
-
-
-
-
-
5
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687599
Lassen
Multiple and additive function ...
Mus musculus
J. Biol. Chem.
282
25668-25676
2007
-
-
-
-
-
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1
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1
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2
2
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4
-
2
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1
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1
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1
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2
2
-
4
-
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-
-
-
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-
677193
Short
Metabolism of trans, trans-muc ...
Mus musculus
Toxicol. Appl. Pharmacol.
210
163-170
2006
-
-
1
-
-
-
-
1
-
-
1
1
-
1
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-
1
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1
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2
-
3
1
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1
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1
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1
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1
1
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1
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1
1
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1
-
1
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2
-
1
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1
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1
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-
655889
Ho
Isolation and characterization ...
Escherichia coli
J. Bacteriol.
187
1067-1073
2005
1
-
1
-
1
-
-
6
-
1
3
2
-
1
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1
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1
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8
1
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1
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2
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1
3
2
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1
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1
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8
1
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1
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-
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-
437840
Dickinson
The purification and some prop ...
Saccharomyces cerevisiae
Biochem. J.
315
393-399
1996
-
-
-
-
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-
2
-
1
1
2
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1
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1
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1
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1
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1
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1
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1
1
2
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1
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1
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1
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2
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-
-
-
437829
Hommel
-
NADP+-dependent aldehyde dehyd ...
Acetobacter pasteurianus, Acetobacter pasteurianus CCM 1774
J. Basic Microbiol.
28
25-33
1988
-
-
-
-
-
-
6
5
1
-
1
2
-
2
-
-
1
1
-
-
1
1
10
-
-
-
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-
1
1
1
1
1
-
-
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-
-
1
-
-
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-
6
1
5
1
-
1
2
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-
-
1
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1
1
10
-
-
-
-
-
1
1
1
-
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-
-
-
-
-
437830
Aurich
Kinetics of membrane-bound ald ...
Acinetobacter calcoaceticus
Biol. Chem. Hoppe-Seyler
368
101-109
1987
1
-
-
-
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-
17
14
2
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1
-
3
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8
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1
1
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1
9
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1
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1
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17
9
14
2
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1
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8
-
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-
-
1
1
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-
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-
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-
437831
Aurich
-
Wechselwirkungen der Aldehydde ...
Acinetobacter calcoaceticus
J. Basic Microbiol.
25
623-629
1985
-
-
-
-
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-
3
1
1
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1
-
1
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-
-
2
-
-
-
-
-
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1
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1
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3
-
1
1
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-
1
-
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2
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-
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-
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-
437832
Aurich
-
Wechselwirkungen der Aldehydde ...
Acinetobacter calcoaceticus
J. Basic Microbiol.
25
631-636
1985
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-
-
-
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1
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1
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1
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1
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1
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1
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1
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1
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1
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348681
Vandecasteele
Aldehyde dehydrogenases from P ...
Pseudomonas aeruginosa
Methods Enzymol.
89
484-490
1982
-
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5
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1
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1
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1
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1
5
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1
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1
-
-
-
-
-
-
5
-
-
1
-
-
-
-
1
-
-
-
1
5
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
437833
Sasaki
Aldehyde dehydrogenase from Pr ...
Proteus vulgaris
Methods Enzymol.
89
480-483
1982
1
-
-
1
-
-
-
4
-
2
1
-
-
1
-
-
1
-
-
-
1
-
4
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
1
-
-
-
-
-
4
-
2
1
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
437834
Muraoka
-
Purification and properties of ...
Acetobacter aceti
J. Ferment. Technol.
58
501-507
1980
-
-
-
-
-
-
11
-
-
3
5
-
-
1
-
-
1
-
-
-
-
-
10
5
-
-
-
2
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
3
5
-
-
-
-
1
-
-
-
-
10
5
-
-
2
-
-
-
1
-
-
-
-
-
-
-
437835
Adachi
-
Crystallization and properties ...
Acetobacter aceti, Acetobacter acetigenus, Acetobacter orleanensis, Acetobacter ascendens, Frateuria aurantia, Gluconobacter oxydans, Acetobacter pasteurianus, Gluconobacter albidus, Gluconobacter cerinus, Gluconobacter gluconicus, Gluconacetobacter liquefaciens, Gluconobacter roseus, Gluconobacter sphaericus
Agric. Biol. Chem.
44
155-164
1980
-
-
-
1
-
-
8
2
-
4
2
12
-
21
-
-
1
-
-
-
1
-
35
1
-
1
-
-
-
1
-
1
14
-
-
-
-
-
-
14
1
-
-
-
8
-
2
-
4
2
12
-
-
-
1
-
-
1
-
35
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
348725
Guerrillot
Purification and characterizat ...
Pseudomonas aeruginosa
Eur. J. Biochem.
81
185-192
1977
-
-
-
-
-
-
3
15
-
1
1
-
-
1
-
-
1
-
-
-
1
1
14
-
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
1
-
-
-
-
3
-
15
-
1
1
-
-
-
-
1
-
-
1
1
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437836
Sugawara
Purification and properties of ...
Proteus vulgaris
Biochim. Biophys. Acta
480
343-350
1977
2
-
-
1
-
-
-
3
-
2
1
-
-
6
-
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1
-
-
-
1
-
4
-
-
-
-
-
-
1
-
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1
-
-
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2
-
-
1
1
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-
-
-
3
-
2
1
-
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-
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1
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
437839
Jacobson
Mitochondrial acetaldehyde deh ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
350
277-291
1974
-
-
-
-
-
-
-
-
1
1
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1
-
1
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-
-
-
-
-
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1
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1
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-
-
-
-
-
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1
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-
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-
1
-
-
-
-
-
-
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1
1
-
1
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-
-
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-
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-
-
1
-
-
-
-
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-
-
-
-
-
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-
-
-
-
437838
Seegmiller
Triphosphopyridine nucleotide- ...
Saccharomyces cerevisiae
J. Biol. Chem.
201
629-637
1953
-
-
-
-
-
-
-
3
-
4
-
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1
-
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1
-
-
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1
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4
-
-
-
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1
1
-
1
-
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-
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1
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-
-
3
-
4
-
-
-
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1
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1
-
4
-
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-
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1
1
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-