Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM causes 5% inhibition at 30°C | Pseudomonas sp. | |
3-methylbutyraldehyde | 1 mM causes 57% inhibition at 30°C | Pseudomonas sp. | |
4-N-trimethylaminobutyraldehyde | the substrate inhibits at 0.24 mM | Pseudomonas sp. | |
5,5'-dithiobis-(2-nitrobenzoic acid) | 0.1 mM causes 100% inhibition at 30°C | Pseudomonas sp. | |
acetaldehyde | 1 mM causes 18% inhibition at 30°C | Pseudomonas sp. | |
Ba2+ | 1 mM causes 30% inhibition at 30°C | Pseudomonas sp. | |
Betaine aldehyde | 1 mM causes 41% inhibition at 30°C | Pseudomonas sp. | |
Co2+ | 1 mM causes 18% inhibition at 30°C | Pseudomonas sp. | |
Cu2+ | 0.05 mM causes 36% inhibition at 30°C | Pseudomonas sp. | |
diethylmethylamine | at 30°C, 5 mM causes 53% inhibition, does not inhibit at 1 mM | Pseudomonas sp. | |
dimethylethylamine | 5 mM causes 9% inhibition at 30°C | Pseudomonas sp. | |
ethylmethylamine | 1 mM causes 20% inhibition at 30°C | Pseudomonas sp. | |
glyceraldehyde | 1 mM causes 7% inhibition at 30°C | Pseudomonas sp. | |
Hg2+ | 0.05 mM causes 28% inhibition at 30°C | Pseudomonas sp. | |
iodoacetoamide | 1 mM causes 42% inhibition at 30°C | Pseudomonas sp. | |
KCl | 1 mM causes 5% inhibition at 30°C | Pseudomonas sp. | |
Li+ | 1 mM causes 13% inhibition at 30°C | Pseudomonas sp. | |
additional information | at 30°C, not inhibited by Rb+, Na+, Mg2+, Ca2+, potassium fluoride, 1 mM sodium azide, 2,2'-dipyridyl, EDTA, 1 mM trimethylamine and 1 mM tetramethylammonium chloride | Pseudomonas sp. | |
p-chloromercuribenzoate | 0.1 mM causes 88% inhibition at 30°C | Pseudomonas sp. | |
phenylacetaldehyde | 0.1 mM causes 54% inhibition at 30°C | Pseudomonas sp. | |
Semicarbazide | 1 mM causes 22% inhibition at 30°C | Pseudomonas sp. | |
triethylamine | 5 mM causes 42% inhibition at 30°C | Pseudomonas sp. | |
trimethylacetaldehyde | 1 mM causes 80% inhibition at 30°C | Pseudomonas sp. | |
Zn2+ | 1 mM causes 11% inhibition at 30°C | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0074 | - |
4-N-trimethylaminobutyraldehyde | - |
Pseudomonas sp. | |
0.051 | - |
4-dimethylaminobutyraldehyde | - |
Pseudomonas sp. | |
0.125 | - |
NAD+ | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
3 * 55000, SDS-PAGE | Pseudomonas sp. |
160000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. 13CM | - |
- |
- |
Purification (Comment) | Organism |
---|---|
14.2fold to apparent homogeneity, by hydrophobic chromatography and affinity chromatography | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.8 | - |
cell-free extract | Pseudomonas sp. |
12.4 | - |
14.2fold purified enzyme | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-dimethylaminobutyraldehyde + NAD+ | - |
Pseudomonas sp. | 4-dimethylaminobutanoate + NADH | - |
? | |
4-dimethylaminobutyraldehyde + NAD+ | - |
Pseudomonas sp. 13CM | 4-dimethylaminobutanoate + NADH | - |
? | |
4-N-trimethylaminobutyraldehyde + NAD+ | - |
Pseudomonas sp. | 4-N-trimethylaminobutanoate + NADH | - |
? | |
4-N-trimethylaminobutyraldehyde + NAD+ | - |
Pseudomonas sp. 13CM | 4-N-trimethylaminobutanoate + NADH | - |
? | |
additional information | does not degradate 4-trimethylaminobutanol, betaine aldehyde, 4-aminobutyraldehyde, acetaldehyde, propionaldehyde, butyraldehyde, trimethylacetaldehyde, and 3-methylbutyraldehyde | Pseudomonas sp. | ? | - |
? | |
additional information | does not degradate 4-trimethylaminobutanol, betaine aldehyde, 4-aminobutyraldehyde, acetaldehyde, propionaldehyde, butyraldehyde, trimethylacetaldehyde, and 3-methylbutyraldehyde | Pseudomonas sp. 13CM | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 55000, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
4-N-trimethylaminobutyraldehyde dehydrogenase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
the enzyme is rapidly inactivated at temperatures above 35°C | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | the enzyme is completely inactivated at pH 9.6 | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | does not react with NADP+ | Pseudomonas sp. | |
NAD+ | is specific for NAD+ | Pseudomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas sp. | isoelectric focusing | - |
5.5 |