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Literature summary for 1.2.1.89 extracted from
- Glyceraldehyde dehydrogenases from the thermoacidophilic euryarchaeota Picrophilus torridus and Thermoplasma acidophilum, key enzymes of the non-phosphorylative Entner-Doudoroff pathway, constitute a novel enzyme family within the aldehyde dehydrogenase superfamily (2006), FEBS Lett., 580, 1198-1204.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Picrophilus torridus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glyceraldehyde | slight substrate inhibition, 25% inhibition at 2 mM | Picrophilus torridus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
D-glyceraldehyde | pH 6.9, 58°C | Picrophilus torridus | |
| 0.2 | - |
D-glyceraldehyde | pH 6.9, 58°C | Thermoplasma acidophilum | |
| 0.36 | - |
NADP+ | pH 6.9, 58°C | Thermoplasma acidophilum | |
| 0.42 | - |
NADP+ | pH 6.9, 58°C | Picrophilus torridus | |
| 5.1 | - |
D-glycerate | pH 6.9, 58°C | Picrophilus torridus | |
| 11.1 | - |
glycolaldehyde | pH 6.9, 58°C | Picrophilus torridus | |
| 12.3 | - |
glycolaldehyde | pH 6.9, 58°C | Thermoplasma acidophilum | |
| 18.3 | - |
D-glycerate | pH 6.9, 58°C | Thermoplasma acidophilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54700 | - |
2 * 54700, calculated from sequence | Thermoplasma acidophilum |
| 55200 | - |
2 * 55200, calculated from sequence | Picrophilus torridus |
| 56000 | - |
2 * 56000, SDS-PAGE | Thermoplasma acidophilum |
| 56000 | - |
2 * 56000, SDS-PAGE | Picrophilus torridus |
| 115000 | - |
gel filtration | Picrophilus torridus |
| 120000 | - |
gel filtration | Thermoplasma acidophilum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde + NADP+ + H2O | Thermoplasma acidophilum | the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde + NADP+ + H2O | Picrophilus torridus | the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde + NADP+ + H2O | Thermoplasma acidophilum JCM 9062 | the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde + NADP+ + H2O | Picrophilus torridus DSM 9790 | the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway | D-glycerate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Picrophilus torridus | Q6L285 | - |
- |
| Picrophilus torridus DSM 9790 | Q6L285 | - |
- |
| Thermoplasma acidophilum | Q9HK01 | - |
- |
| Thermoplasma acidophilum JCM 9062 | Q9HK01 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoplasma acidophilum |
- |
Picrophilus torridus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6 | - |
pH 6.9, 58°C | Picrophilus torridus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde + NADP+ + H2O | the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway | Thermoplasma acidophilum | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde + NADP+ + H2O | the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway | Picrophilus torridus | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde + NADP+ + H2O | completely inactive with NAD+ | Thermoplasma acidophilum | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde + NADP+ + H2O | completely inactive with NAD+ | Picrophilus torridus | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde + NADP+ + H2O | the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway | Thermoplasma acidophilum JCM 9062 | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde + NADP+ + H2O | completely inactive with NAD+ | Thermoplasma acidophilum JCM 9062 | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde + NADP+ + H2O | the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway | Picrophilus torridus DSM 9790 | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde + NADP+ + H2O | completely inactive with NAD+ | Picrophilus torridus DSM 9790 | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde 3-phosphate + NADP+ + H2O | catalytic efficiency (kcat/Km) for D-glyceraldehyde 3-phosphate is 1.4% compared to the catalytic efficiency with D-glyceraldehyde | Picrophilus torridus | D-glycerate 3-phosphate + NADPH + H+ | - |
? | |
| D-glyceraldehyde 3-phosphate + NADP+ + H2O | catalytic efficiency (kcat/Km) for glyceraldehyde 3-phosphate is less than 1% compared to the catalytic efficiency with D-glyceraldehyde | Thermoplasma acidophilum | D-glycerate 3-phosphate + NADPH + H+ | - |
? | |
| D-glyceraldehyde 3-phosphate + NADP+ + H2O | catalytic efficiency (kcat/Km) for glyceraldehyde 3-phosphate is less than 1% compared to the catalytic efficiency with D-glyceraldehyde | Thermoplasma acidophilum JCM 9062 | D-glycerate 3-phosphate + NADPH + H+ | - |
? | |
| D-glyceraldehyde 3-phosphate + NADP+ + H2O | catalytic efficiency (kcat/Km) for D-glyceraldehyde 3-phosphate is 1.4% compared to the catalytic efficiency with D-glyceraldehyde | Picrophilus torridus DSM 9790 | D-glycerate 3-phosphate + NADPH + H+ | - |
? | |
| glycolaldehyde + NADP+ | catalytic efficiency (kcat/Km) for glycolaldehyde is 1% compared to the catalytic efficiency with D-glyceraldehyde | Picrophilus torridus | glycolate + NADPH + H+ | - |
? | |
| glycolaldehyde + NADP+ | catalytic efficiency (kcat/Km) for glycolaldehyde is about 2% compared to the catalytic efficiency with D-glyceraldehyde | Thermoplasma acidophilum | glycolate + NADPH + H+ | - |
? | |
| glycolaldehyde + NADP+ | catalytic efficiency (kcat/Km) for glycolaldehyde is about 2% compared to the catalytic efficiency with D-glyceraldehyde | Thermoplasma acidophilum JCM 9062 | glycolate + NADPH + H+ | - |
? | |
| glycolaldehyde + NADP+ | catalytic efficiency (kcat/Km) for glycolaldehyde is 1% compared to the catalytic efficiency with D-glyceraldehyde | Picrophilus torridus DSM 9790 | glycolate + NADPH + H+ | - |
? | |
| additional information | no activity with: acetaldehyde, crotonaldehyde, benzaldehyde, succinate semialdehyde, and dihydroxyacetone | Picrophilus torridus | ? | - |
? | |
| additional information | no activity with: acetaldehyde, crotonaldehyde, benzaldehyde, succinate semialdehyde, and dihydroxyacetone | Picrophilus torridus DSM 9790 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 56000, SDS-PAGE | Thermoplasma acidophilum |
| homodimer | 2 * 56000, SDS-PAGE | Picrophilus torridus |
| homodimer | 2 * 54700, calculated from sequence | Thermoplasma acidophilum |
| homodimer | 2 * 55200, calculated from sequence | Picrophilus torridus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GADH | - |
Thermoplasma acidophilum |
| GADH | - |
Picrophilus torridus |
| Pto0332 | locus name | Picrophilus torridus |
| Ta0809 | locus name | Thermoplasma acidophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
assay at | Thermoplasma acidophilum |
| 58 | - |
assay at | Picrophilus torridus |
| 63 | - |
- |
Thermoplasma acidophilum |
| 63 | - |
- |
Picrophilus torridus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
2 h, stable | Thermoplasma acidophilum |
| 60 | - |
2 h, stable | Picrophilus torridus |
| 70 | - |
half-life: 15 min | Picrophilus torridus |
| 70 | - |
half-life: 40 min | Thermoplasma acidophilum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
NADP+ | pH 6.9, 58°C | Picrophilus torridus | |
| 10 | - |
D-glyceraldehyde | pH 6.9, 58°C | Picrophilus torridus | |
| 12 | - |
D-glycerate | pH 6.9, 58°C | Picrophilus torridus | |
| 20 | - |
D-glycerate | pH 6.9, 58°C | Thermoplasma acidophilum | |
| 20 | - |
glycolaldehyde | pH 6.9, 58°C | Picrophilus torridus | |
| 25 | - |
NADP+ | pH 6.9, 58°C | Thermoplasma acidophilum | |
| 26 | - |
D-glyceraldehyde | pH 6.9, 58°C | Thermoplasma acidophilum | |
| 37 | - |
glycolaldehyde | pH 6.9, 58°C | Thermoplasma acidophilum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
- |
Picrophilus torridus |
| 6.9 | - |
assay at | Picrophilus torridus |
| 7.6 | - |
- |
Thermoplasma acidophilum |
| 7.9 | - |
assay at | Thermoplasma acidophilum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | 8.1 | pH 4.3: about 50% of maximal activity, pH 8.1: about 50% of maximal activity | Picrophilus torridus |
| 5.8 | 8.7 | pH 5.8: about 50% of maximal activity, pH 8.7: about 50% of maximal activity | Thermoplasma acidophilum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | completely inactive with NAD+ | Thermoplasma acidophilum | |
| NADP+ | completely inactive with NAD+ | Picrophilus torridus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway | Thermoplasma acidophilum |
| physiological function | the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway | Picrophilus torridus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
D-glycerate | pH 6.9, 58°C | Thermoplasma acidophilum | |
| 1.8 | - |
glycolaldehyde | pH 6.9, 58°C | Picrophilus torridus | |
| 2.4 | - |
D-glycerate | pH 6.9, 58°C | Picrophilus torridus | |
| 3 | - |
glycolaldehyde | pH 6.9, 58°C | Thermoplasma acidophilum | |
| 24 | - |
NADP+ | pH 6.9, 58°C | Picrophilus torridus | |
| 70 | - |
NADP+ | pH 6.9, 58°C | Thermoplasma acidophilum | |
| 130 | - |
D-glyceraldehyde | pH 6.9, 58°C | Thermoplasma acidophilum | |
| 167 | - |
D-glyceraldehyde | pH 6.9, 58°C | Picrophilus torridus | |
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