Any feedback?
Information on EC 1.2.1.89 - D-glyceraldehyde dehydrogenase (NADP+)
for references in articles please use BRENDA:EC1.2.1.89Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
1.2.1.89 D-glyceraldehyde dehydrogenase (NADP+)IUBMB Comments
The enzyme from the archaea Thermoplasma acidophilum and Picrophilus torridus is involved in the non-phosphorylative Entner-Doudoroff pathway. cf. EC 1.2.99.8, glyceraldehyde dehydrogenase (FAD-containing).
Specify your search results
Word Map
- 1.2.1.89
- enolase
-
thermoplasma
- acidophilum
-
entner-doudoroff
- beta-sitosterol
- stigmasterol
- synthesis
The enzyme appears in viruses and cellular organisms
Synonyms
glyceraldehyde dehydrogenase, ta0809, pto0332, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ALDH
GADH
GLD
glyceraldehyde dehydrogenase
Pto0332
Ta0809
TaAlDH
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
D-glyceraldehyde + NADP+ + H2O = D-glycerate + NADPH + H+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
BRENDA
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
D-glyceraldehyde:NADP+ oxidoreductase
The enzyme from the archaea Thermoplasma acidophilum and Picrophilus torridus is involved in the non-phosphorylative Entner-Doudoroff pathway. cf. EC 1.2.99.8, glyceraldehyde dehydrogenase (FAD-containing).
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-glyceraldehyde + NAD+
D-glycerate + NADH + H+
NAD+ is a very poor cofactor for wild-type
-
-
?
D-glyceraldehyde + NAD+ + H2O
D-glycerate + NADH + H+
activity of mutants N286E, N286H, N286T. No substrate for wild-type
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
completely inactive with NAD+
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
completely inactive with NAD+
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
the enzyme is involved in downstream portion of the non-phosphorylated EntnerDoudoroff pathway
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
also accepts NAD+ under technically relevant conditions, cofactor above 1 mM
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
completely inactive when NAD+ is substituted for NADP+
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
completely inactive with NAD+
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
the enzyme is involved in downstream portion of the non-phosphorylated EntnerDoudoroff pathway
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
completely inactive when NAD+ is substituted for NADP+
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
completely inactive with NAD+
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
also accepts NAD+ under technically relevant conditions, cofactor above 1 mM
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
D-glycerate 3-phosphate + NADPH + H+
catalytic efficiency (kcat/Km) for D-glyceraldehyde 3-phosphate is 1.4% compared to the catalytic efficiency with D-glyceraldehyde
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
D-glycerate 3-phosphate + NADPH + H+
catalytic efficiency (kcat/Km) for D-glyceraldehyde 3-phosphate is 1.4% compared to the catalytic efficiency with D-glyceraldehyde
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
D-glycerate 3-phosphate + NADPH + H+
activity is 33% compared to the activity with D-glyceraldehyde
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
D-glycerate 3-phosphate + NADPH + H+
catalytic efficiency (kcat/Km) for glyceraldehyde 3-phosphate is less than 1% compared to the catalytic efficiency with D-glyceraldehyde
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
D-glycerate 3-phosphate + NADPH + H+
activity is 33% compared to the activity with D-glyceraldehyde
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
D-glycerate 3-phosphate + NADPH + H+
catalytic efficiency (kcat/Km) for glyceraldehyde 3-phosphate is less than 1% compared to the catalytic efficiency with D-glyceraldehyde
-
-
?
glycolaldehyde + NADP+
glycolate + NADPH + H+
catalytic efficiency (kcat/Km) for glycolaldehyde is 1% compared to the catalytic efficiency with D-glyceraldehyde
-
-
?
glycolaldehyde + NADP+
glycolate + NADPH + H+
catalytic efficiency (kcat/Km) for glycolaldehyde is 1% compared to the catalytic efficiency with D-glyceraldehyde
-
-
?
glycolaldehyde + NADP+
glycolate + NADPH + H+
activity is 14% compared to the activity with D-glyceraldehyde
-
-
?
glycolaldehyde + NADP+
glycolate + NADPH + H+
catalytic efficiency (kcat/Km) for glycolaldehyde is about 2% compared to the catalytic efficiency with D-glyceraldehyde
-
-
?
glycolaldehyde + NADP+
glycolate + NADPH + H+
activity is 14% compared to the activity with D-glyceraldehyde
-
-
?
glycolaldehyde + NADP+
glycolate + NADPH + H+
catalytic efficiency (kcat/Km) for glycolaldehyde is about 2% compared to the catalytic efficiency with D-glyceraldehyde
-
-
?
additional information
?
-
no activity with: acetaldehyde, crotonaldehyde, benzaldehyde, succinate semialdehyde, and dihydroxyacetone
-
-
?
additional information
?
-
-
no activity with: acetaldehyde, crotonaldehyde, benzaldehyde, succinate semialdehyde, and dihydroxyacetone
-
-
?
additional information
?
-
no activity with: acetaldehyde, crotonaldehyde, benzaldehyde, succinate semialdehyde, and dihydroxyacetone
-
-
?
additional information
?
-
no activity towards ketoaldehyde (methylglyoxal), alkyl aldehydes (formaldehyde, acetaldehyde and propionaldehyde) or aromatic aldehydes (phenylacetaldehyde and 4-nitrobenzaldehyde)
-
-
?
additional information
?
-
-
no activity towards ketoaldehyde (methylglyoxal), alkyl aldehydes (formaldehyde, acetaldehyde and propionaldehyde) or aromatic aldehydes (phenylacetaldehyde and 4-nitrobenzaldehyde)
-
-
?
additional information
?
-
no activity with acetaldehyde, propionaldehyde, n-butyraldehyde, pyruvaldehyde, isobutyraldehyde or D-glucose
-
-
?
additional information
?
-
-
no activity with acetaldehyde, propionaldehyde, n-butyraldehyde, pyruvaldehyde, isobutyraldehyde or D-glucose
-
-
?
additional information
?
-
no activity with acetaldehyde and isobutyraldehyde
-
-
?
additional information
?
-
NAD+ is a poor substrate
-
-
-
additional information
?
-
no activity towards ketoaldehyde (methylglyoxal), alkyl aldehydes (formaldehyde, acetaldehyde and propionaldehyde) or aromatic aldehydes (phenylacetaldehyde and 4-nitrobenzaldehyde)
-
-
?
additional information
?
-
no activity with acetaldehyde, propionaldehyde, n-butyraldehyde, pyruvaldehyde, isobutyraldehyde or D-glucose
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
the enzyme is involved in downstream portion of the non-phosphorylated EntnerDoudoroff pathway
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
the enzyme is involved in downstream portion of the non-phosphorylated EntnerDoudoroff pathway
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH + H+
the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
mutants N286E, N286H, N286T, but not wild-type show activtiy with NAD+
NADP+
also accepts NAD+ under technically relevant conditions, cofactor above 1 mM, Km for NADP+: 0.022 mM, Km for NAD+ is 22.13 mM, at H 6.2, 50°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
D-glyceraldehyde
slight substrate inhibition, 25% inhibition at 2 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4-chloromercuribenzoic acid
1 mM, completely inhibits activity
4-hydroxymercuribenzoic acid
1 mM, completely inhibits activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Ischemic Attack, Transient
Vascular endothelial growth factor gene expression in middle cerebral artery occlusion in the rat.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
10.54
NAD+
mutant enzyme F34M/Y399C/S405N, at pH 7.0 and 50°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.3 - 8.1
pH 4.3: about 50% of maximal activity, pH 8.1: about 50% of maximal activity
5.8 - 8.7
pH 5.8: about 50% of maximal activity, pH 8.7: about 50% of maximal activity
6 - 10
pH 6.0: about 65% of maximal activity, pH 10.0: about 70% of maximal activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
58
63
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30 - 70
30°C: about 50% of maximal activity, 70°C: about 60% of maximal activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
the enzyme is involved in downstream portion of the non-phosphorylated EntnerDoudoroff pathway
physiological function
the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway
physiological function
the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway
physiological function
-
the enzyme is involved in downstream portion of the non-phosphorylated EntnerDoudoroff pathway
-
physiological function
-
the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway
-
physiological function
-
the enzyme is involved in the non-phosphorylative EntnerDoudoroff pathway
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). GADH_PICTO
Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
493
0
55163
Swiss-Prot
-
GADH_THEAC
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
493
0
54782
Swiss-Prot
-
A0A7G9YGG0_9EURY
468
0
50691
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PDB
SCOP
CATH
UNIPROT
ORGANISM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
56000
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.