BRENDA - Enzyme Database
show all sequences of 1.2.1.90

The crystal structure of the allosteric non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeum Thermoproteus tenax

Pohl, E.; Brunner, N.; Wilmanns, M.; Hensel, R.; J. Biol. Chem. 277, 19938-19945 (2002)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
hanging drop vapor diffusion method. Crystal structure of of the complex of the enzyme with its natural inhibitor NADP+. The structure is solved by multiple anomalous diffraction and refined to a resolution of 2.4 A with a crystallographic R-factor of 0.21
Thermoproteus tenax
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-glyceraldehyde 3-phosphate + NAD+ + H2O
Thermoproteus tenax
-
3-phospho-D-glycerate + NADH + 2 H+
-
-
ir
Organism
Organism
UniProt
Commentary
Textmining
Thermoproteus tenax
O57693
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Thermoproteus tenax
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-glyceraldehyde 3-phosphate + NAD+ + H2O
-
288349
Thermoproteus tenax
3-phospho-D-glycerate + NADH + 2 H+
-
-
-
ir
Synonyms
Synonyms
Commentary
Organism
GAPN
-
Thermoproteus tenax
NAD+-dependent non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Thermoproteus tenax
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method. Crystal structure of of the complex of the enzyme with its natural inhibitor NADP+. The structure is solved by multiple anomalous diffraction and refined to a resolution of 2.4 A with a crystallographic R-factor of 0.21
Thermoproteus tenax
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-glyceraldehyde 3-phosphate + NAD+ + H2O
Thermoproteus tenax
-
3-phospho-D-glycerate + NADH + 2 H+
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermoproteus tenax
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-glyceraldehyde 3-phosphate + NAD+ + H2O
-
288349
Thermoproteus tenax
3-phospho-D-glycerate + NADH + 2 H+
-
-
-
ir
Other publictions for EC 1.2.1.90
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728132
Lorentzen
Structural basis of allosteric ...
Thermoproteus tenax
J. Mol. Biol.
341
815-828
2004
4
-
1
1
-
-
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
3
-
2
1
-
-
-
1
-
-
2
-
-
-
4
-
1
2
1
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
288349
Pohl
The crystal structure of the a ...
Thermoproteus tenax
J. Biol. Chem.
277
19938-19945
2002
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
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1
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727454
Brunner
Role of two different glyceral ...
Thermoproteus tenax
Extremophiles
5
101-109
2001
-
-
-
-
-
-
-
4
-
-
-
1
-
1
-
-
-
-
-
2
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
727825
Brunner
NAD+-dependent glyceraldehyde- ...
Thermoproteus tenax
J. Biol. Chem.
273
6149-6156
1998
7
-
1
-
-
-
5
3
-
1
2
1
-
1
-
-
1
-
-
-
-
-
2
1
1
1
-
1
-
1
-
-
1
1
-
-
7
-
1
1
-
-
-
-
5
1
3
-
1
2
1
-
-
-
1
-
-
-
-
2
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-