Literature summary for 1.2.7.1 extracted from

Witt, A.; Pozzi, R.; Diesch, S.; Haedicke, O.; Grammel, H.
New light on ancient enzymes - in vitro CO2 fixation by pyruvate synthase of Desulfovibrio africanus and Sulfolobus acidocaldarius (2019), FEBS J., 286, 4494-4508 .

Search for more literature for 1.2.7.1

Application

Application	Comment	Organism
analysis	biochemical assay for activity, low-potential electrons are introduced by photochemical reduction of EDTA/deazaflavin and the generated pyruvate is trapped by chemical derivatization with semicarbazide. The product of CO2 fixation can be detected as pyruvate semicarbazone by HPLC-MS	Desulfocurvibacter africanus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Desulfocurvibacter africanus

Organism

Organism	UniProt	Comment	Textmining
Desulfocurvibacter africanus	P94692	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
72	-	acceptor methyl viologen, pH 9, 40°C	Desulfocurvibacter africanus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+	-	Desulfocurvibacter africanus	pyruvate + CoA + 2 oxidized ferredoxin	-	r
acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+	-	Desulfocurvibacter africanus	pyruvate + CoA + 2 oxidized methyl viologen	-	r
additional information	enzyme is able to use low-potential ferredoxins of the green sulfur bacterium Chlorobium tepidum and of Sulfolobus acidocaldarius whereas CO2 fixation is not supported by the native ferredoxin of Desulfocurvibacter africanus. Methyl viologen as an artificial electron carrier also allows CO2 fixation	Desulfocurvibacter africanus	?	-	-

Subunits

Subunits	Comment	Organism
?	x * 130000, calculated from sequence, x * 128000, SDS-PAGE	Desulfocurvibacter africanus

Synonyms

Synonyms	Comment	Organism
PFOR	-	Desulfocurvibacter africanus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	55	-	Desulfocurvibacter africanus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Desulfocurvibacter africanus

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Release 2023.1 (January 2023)