Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-methionine | maximal activity at 0.5 mM | Cereibacter sphaeroides | |
S-adenosyl-L-methionine | can replace NADP+-NADH in anaerobic reaction | Chromatium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 90% inhibition of anaerobic reaction at 0.17 mM | Cereibacter sphaeroides | |
1,4-Naphthoquinone | 61% inhibition at 0.015 mM | Cereibacter sphaeroides | |
2,2-bipyridyl | 77% inhibition of anaerobic reaction at 1.5 mM | Cereibacter sphaeroides | |
2,4-dinitrophenol | 95% inhibition of anaerobic reaction at 1 mM | Cereibacter sphaeroides | |
FAD | 95% inhibition of anaerobic reaction at 0.03 mM | Cereibacter sphaeroides | |
FMN | 95% inhibition of anaerobic reaction at 0.03 mM | Cereibacter sphaeroides | |
riboflavin | 95% inhibition of anaerobic reaction at 0.03 mM | Cereibacter sphaeroides | |
S-adenosylethionine | marked inhibition of anaerobic reaction | Cereibacter sphaeroides | |
S-adenosylhomocysteine | marked inhibition of anaerobic reaction | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | 0.05 | Coproporphyrinogen-III | - |
Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
gel filtration | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen-III + ? | Cereibacter sphaeroides | anaerobic enzyme, presence of Mg2+, L-Met, ATP, NADH and NADP+ | protoporphyrinogen-IX + ? | - |
? | |
coproporphyrinogen-III + ? | Chromatium sp. | anaerobic enzyme, presence of Mg2+, L-Met, ATP, NADH and NADP+ | protoporphyrinogen-IX + ? | - |
? | |
coproporphyrinogen-III + ? | Chromatium sp. D | anaerobic enzyme, presence of Mg2+, L-Met, ATP, NADH and NADP+ | protoporphyrinogen-IX + ? | - |
? | |
coproporphyrinogen-III + O2 + 2 H+ | Cereibacter sphaeroides | - |
protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? | |
coproporphyrinogen-III + O2 + 2 H+ | Chromatium sp. | - |
protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? | |
coproporphyrinogen-III + O2 + 2 H+ | Chromatium sp. D | - |
protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Chromatium sp. | - |
- |
- |
Chromatium sp. D | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Cereibacter sphaeroides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Cereibacter sphaeroides | - |
cell culture | - |
Chromatium sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen-III + ? | anaerobic enzyme, presence of Mg2+, L-Met, ATP, NADH and NADP+ | Cereibacter sphaeroides | protoporphyrinogen-IX + ? | - |
? | |
coproporphyrinogen-III + ? | anaerobic enzyme, presence of Mg2+, L-Met, ATP, NADH and NADP+ | Chromatium sp. | protoporphyrinogen-IX + ? | - |
? | |
coproporphyrinogen-III + ? | anaerobic enzyme, presence of Mg2+, L-Met, ATP, NADH and NADP+ | Chromatium sp. D | protoporphyrinogen-IX + ? | - |
? | |
coproporphyrinogen-III + O2 + 2 H+ | - |
Cereibacter sphaeroides | protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? | |
coproporphyrinogen-III + O2 + 2 H+ | - |
Chromatium sp. | protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? | |
coproporphyrinogen-III + O2 + 2 H+ | - |
Chromatium sp. D | protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
aerobic activity | Cereibacter sphaeroides |
8.5 | - |
anaerobic reaction | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Chromatium sp. | |
ATP | strictly required for anaerobic enzyme | Cereibacter sphaeroides | |
NADP+ | only anaerobic enzyme, maximum activity together with NADH | Cereibacter sphaeroides | |
NADP+ | only anaerobic enzyme, maximum activity together with NADH | Chromatium sp. |