Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D400A | mutation severely impaires the catalytic activity, mutant performs, on average, less than a single turnover | Homo sapiens |
R262A | mutation results in decreased kcat values and increased Km values. No product formation is detected with sunstrate mesoporphyrogen-VI | Homo sapiens |
R401A | mutation results in decreased kcat values and increased Km values. The mutant is able to convert mesoporphyrogen-VI to monovinyl product, although over 10fold less efficiently than wild-type | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00034 | - |
Coproporphyrinogen-III | wild-type, divinyl product, 37°C, pH not specified in the publication | Homo sapiens | |
0.00056 | - |
mesoporphyrinogen-VI | wild-type, monovinyl product, 37°C, pH not specified in the publication | Homo sapiens | |
0.00078 | - |
Coproporphyrinogen-III | mutant D400A, total product, 37°C, pH not specified in the publication | Homo sapiens | |
0.00079 | - |
Coproporphyrinogen-III | wild-type, total product, 37°C, pH not specified in the publication | Homo sapiens | |
0.00096 | - |
Coproporphyrinogen-III | mutant R401A, divinyl product, 37°C, pH not specified in the publication | Homo sapiens | |
0.002 | - |
mesoporphyrinogen-VI | mutant R401A, monovinyl product, 37°C, pH not specified in the publication | Homo sapiens | |
0.0029 | - |
Coproporphyrinogen-III | mutant R401A, total product, 37°C, pH not specified in the publication | Homo sapiens | |
0.0051 | - |
Coproporphyrinogen-III | mutant R262A, divinyl product, 37°C, pH not specified in the publication | Homo sapiens | |
0.0087 | - |
Coproporphyrinogen-III | mutant R262A, total product, 37°C, pH not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P36551 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, Ni-NTA agarose metal affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen-III + O2 + 2 H+ | - |
Homo sapiens | protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? | |
harderoporphyrinogen + O2 + 2 H+ | substrate undergoes only the second decarboxylation | Homo sapiens | protoporphyrinogen + CO2 + H2O | - |
? | |
mesoporphyrinogen-VI + O2 + H+ | substrate undergoes only the first oxidative decarboxylation | Homo sapiens | ? + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CPOX | - |
Homo sapiens |
oxygen-dependent coproporphyrinogen-III oxidase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000002 | - |
Coproporphyrinogen-III | mutant D400A, total product, 37°C, pH not specified in the publication | Homo sapiens | |
0.000012 | - |
Coproporphyrinogen-III | mutant R262A, divinyl product, 37°C, pH not specified in the publication | Homo sapiens | |
0.00042 | - |
Coproporphyrinogen-III | mutant R262A, total product, 37°C, pH not specified in the publication | Homo sapiens | |
0.00167 | - |
Coproporphyrinogen-III | mutant R401A, divinyl product, 37°C, pH not specified in the publication | Homo sapiens | |
0.002 | - |
mesoporphyrinogen-VI | mutant R401A, monovinyl product, 37°C, pH not specified in the publication | Homo sapiens | |
0.0063 | - |
mesoporphyrinogen-VI | wild-type, monovinyl product, 37°C, pH not specified in the publication | Homo sapiens | |
0.02 | - |
Coproporphyrinogen-III | mutant R401A, total product, 37°C, pH not specified in the publication | Homo sapiens | |
0.02667 | - |
Coproporphyrinogen-III | wild-type, divinyl product, 37°C, pH not specified in the publication | Homo sapiens | |
0.045 | - |
Coproporphyrinogen-III | wild-type, total product, 37°C, pH not specified in the publication | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000003 | - |
Coproporphyrinogen-III | mutant R262A, divinyl product, 37°C, pH not specified in the publication | Homo sapiens | |
0.000027 | - |
Coproporphyrinogen-III | mutant D400A, total product, 37°C, pH not specified in the publication | Homo sapiens | |
0.000048 | - |
Coproporphyrinogen-III | mutant R262A, total product, 37°C, pH not specified in the publication | Homo sapiens | |
0.001 | - |
mesoporphyrinogen-VI | mutant R401A, monovinyl product, 37°C, pH not specified in the publication | Homo sapiens | |
0.00183 | - |
Coproporphyrinogen-III | mutant R401A, divinyl product, 37°C, pH not specified in the publication | Homo sapiens | |
0.00683 | - |
Coproporphyrinogen-III | mutant R401A, total product, 37°C, pH not specified in the publication | Homo sapiens | |
0.0113 | - |
mesoporphyrinogen-VI | wild-type, monovinyl product, 37°C, pH not specified in the publication | Homo sapiens | |
0.0567 | - |
Coproporphyrinogen-III | wild-type, total product, 37°C, pH not specified in the publication | Homo sapiens | |
0.07 | - |
Coproporphyrinogen-III | wild-type, divinyl product, 37°C, pH not specified in the publication | Homo sapiens |