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Literature summary for 1.3.3.5 extracted from

  • Sadeghian, I.; Rezaie, Z.; Rahmatabadi, S.; Hemmati, S.
    Biochemical insights into a novel thermo/organo tolerant bilirubin oxidase from Thermosediminibacter oceani and its application in dye decolorization (2020), Process Biochem., 88, 38-50 .
No PubMed abstract available
Search for more literature for 1.3.3.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermosediminibacter oceani

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 15.21% residual activity at 5 mM Thermosediminibacter oceani
acetone 81.18% residual activity at 50% (v/v) Thermosediminibacter oceani
acetonitrile 69.02% residual activity at 50% (v/v) Thermosediminibacter oceani
Ca2+ 84.91% residual activity at 10 mM Thermosediminibacter oceani
dimethyl sulfoxide 68.04% residual activity at 50% (v/v) Thermosediminibacter oceani
dimethylformamide 55.72% residual activity at 50% (v/v) Thermosediminibacter oceani
dithiothreitol 1.32% residual activity at 0.1 mM Thermosediminibacter oceani
EDTA 21.11% residual activity at 5 mM Thermosediminibacter oceani
ethanol 70.42% residual activity at 50% (v/v) Thermosediminibacter oceani
Fe2+ 56.04% residual activity at 5 mM Thermosediminibacter oceani
Fe3+ 52.1% residual activity at 5 mM Thermosediminibacter oceani
K+ 98.25% residual activity at 10 mM Thermosediminibacter oceani
methanol 78.29% residual activity at 50% (v/v) Thermosediminibacter oceani
Mg2+ 85.36% residual activity at 10 mM Thermosediminibacter oceani
Mn2+ 65.77% residual activity at 10 mM Thermosediminibacter oceani
Na+ 94.47% residual activity at 10 mM Thermosediminibacter oceani
SDS 41.42% residual activity at 0.1 mM Thermosediminibacter oceani
Sodium azide complete inhibition at 5 mM Thermosediminibacter oceani
Triton X-100 98.75% residual activity at 5 mM Thermosediminibacter oceani
Zn2+ 80.19% residual activity at 10 mM Thermosediminibacter oceani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0196
-
 syringaldazine at pH 6.5 and 70°C Thermosediminibacter oceani
0.0312
-
 bilirubin at pH 8.0 and 70°C Thermosediminibacter oceani
0.1265
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.5 and 70°C Thermosediminibacter oceani

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 112.68% activity at 5 mM Thermosediminibacter oceani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 bilirubin + O2 Thermosediminibacter oceani
-
 2 biliverdin + H2O
-
 ?
2 bilirubin + O2 Thermosediminibacter oceani DSM 16646
-
 2 biliverdin + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Thermosediminibacter oceani D9RY72
-
-
Thermosediminibacter oceani DSM 16646 D9RY72
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Thermosediminibacter oceani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 bilirubin + O2
-
 Thermosediminibacter oceani 2 biliverdin + H2O
-
 ?
2 bilirubin + O2
-
 Thermosediminibacter oceani DSM 16646 2 biliverdin + H2O
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Thermosediminibacter oceani oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Thermosediminibacter oceani DSM 16646 oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
 ?
additional information in the presence of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as the mediator, the enzyme decolorizes crystal violet, malachite green and Congo red dyes at a rate of about 36%, 63% and 71%, respectively in 2 h Thermosediminibacter oceani ?
-
-
additional information in the presence of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as the mediator, the enzyme decolorizes crystal violet, malachite green and Congo red dyes at a rate of about 36%, 63% and 71%, respectively in 2 h Thermosediminibacter oceani DSM 16646 ?
-
-
syringaldazine + O2
-
 Thermosediminibacter oceani oxidized syringaldazine + H2O
-
 ?
syringaldazine + O2
-
 Thermosediminibacter oceani DSM 16646 oxidized syringaldazine + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 66000, SDS-PAGE Thermosediminibacter oceani
monomer 1 * 64600, calculated from amino acid sequence Thermosediminibacter oceani

Synonyms

Synonyms Comment Organism
BOD
-
 Thermosediminibacter oceani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Thermosediminibacter oceani

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 95 more than 58% activity between 55 and 95°C Thermosediminibacter oceani

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 90 at 40°C and 50°C, the enzyme retains 100% of its initial activity after 180 min of incubation. At 60°C, the enzyme shows approximately 100% of its maximum activity for 60 min. The enzyme has a half-life of 180 min at 70°C and 120 min at 80°C, respectively. At 90°C, the enzyme shows 85% of its initial activity after 30 min of incubation and the enzyme's half-life at this temperature is about 60 min. Moreover, the enzyme retains 39%, 29%, and 15% of its initial activity after incubation at 90°C for 90 min, 120 min, and 180 min, respectively Thermosediminibacter oceani

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
72.5
-
 syringaldazine at pH 6.5 and 70°C Thermosediminibacter oceani
76.2
-
 bilirubin at pH 8.0 and 70 °C Thermosediminibacter oceani
130.9
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.5 and 70°C Thermosediminibacter oceani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 pH optimum with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as substrate Thermosediminibacter oceani
6.5
-
 pH optimum with syringaldazine as substrate Thermosediminibacter oceani
8
-
 pH optimum with bilirubin as substrate Thermosediminibacter oceani

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 10 the enzyme exhibits high stability over a pH range from 3.5 to 10.0, retaining 71% of its original activity after incubation at 4°C for 24 h Thermosediminibacter oceani

pI Value

Organism Comment pI Value Maximum pI Value
Thermosediminibacter oceani calculated from amino acid sequence
-
 7.3