Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-aminoorotate | - |
Plasmodium falciparum | |
5-bromoorotate | - |
Plasmodium falciparum | |
5-Fluoroorotate | - |
Plasmodium falciparum | |
5-iodoorotate | - |
Plasmodium falciparum | |
5-Methylorotate | - |
Plasmodium falciparum | |
atovaquone | - |
Plasmodium falciparum | |
benzoquinones | ubiquinone-0 and decylubiquinone | Plasmodium falciparum | |
chloronaphthoquinone | - |
Plasmodium falciparum | |
Lapachol | - |
Plasmodium falciparum | |
Lawsone | - |
Plasmodium falciparum | |
menadione | - |
Plasmodium falciparum | |
Orotate | also inhibitors: 5-substituted orotates; competitive to dihydroorotate; strong competitive inhibitor | Plasmodium falciparum | |
rhodamine 123 | mitochondrial inhibitor | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
dihydroorotate | - |
Plasmodium falciparum | |
0.0144 | - |
S-dihydroorotate | - |
Plasmodium falciparum | |
0.022 | - |
coenzyme Q6 | - |
Plasmodium falciparum | |
0.0225 | - |
ubiquinone-6 | - |
Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Plasmodium falciparum | 5739 | - |
mitochondrion | inner membrane | Plasmodium falciparum | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
gel filtration | Plasmodium falciparum |
56000 | - |
gel filtration, SDS-PAGE | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + ubiquinone | Plasmodium falciparum | fourth step in synthesis of uridine 5'-monophosphate | orotate + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Plasmodium falciparum | - |
mature trophozytes and schizonts | - |
Purification (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
cation-, anion-exchange, gel filtration | Plasmodium falciparum |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0002672 | - |
37°C | Plasmodium falciparum |
Storage Stability | Organism |
---|---|
-80°C, addition of S-dihydroorotate to stabilize the enzyme activity | Plasmodium falciparum |
4°C, extremely labile, 90% decrease of activity over night | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + ubiquinone | ubiquinone-6, ubiquinone-7, ubiquinone-9 and ubiquinone-10 maximize enzyme activity | Plasmodium falciparum | orotate + ubiquinol | - |
? | |
(S)-dihydroorotate + ubiquinone | fourth step in synthesis of uridine 5'-monophosphate | Plasmodium falciparum | orotate + ubiquinol | - |
? | |
dihydroorotate + acceptor | acceptor: quinones | Plasmodium falciparum | orotate + reduced acceptor | - |
? | |
L-dihydroorotate + ubiquinone-6 | - |
Plasmodium falciparum | orotate + reduced ubiquinone-6 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 56000, SDS-PAGE | Plasmodium falciparum |
monomer | SDS-PAGE | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
S-dihydroorotate | - |
Plasmodium falciparum | |
0.36 | - |
ubiquinone6 | - |
Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0151 | - |
5-Fluoroorotate | - |
Plasmodium falciparum | |
0.018 | - |
Orotate | - |
Plasmodium falciparum | |
0.0182 | - |
Orotate | - |
Plasmodium falciparum | |
0.0244 | - |
5-aminoorotate | - |
Plasmodium falciparum | |
0.1185 | - |
5-Methylorotate | - |
Plasmodium falciparum | |
0.1408 | - |
5-bromoorotate | - |
Plasmodium falciparum | |
0.2246 | - |
5-iodoorotate | - |
Plasmodium falciparum |