Literature summary for 1.3.5.2 extracted from

  • Krungkrai, J.
    Purification, characterization and localization of mitochondrial dihydroorotate dehydrogenase in Plasmodium falciparum, human malaria parasite (1995), Biochim. Biophys. Acta, 1243, 351-360.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5-aminoorotate
-
Plasmodium falciparum
5-bromoorotate
-
Plasmodium falciparum
5-Fluoroorotate
-
Plasmodium falciparum
5-iodoorotate
-
Plasmodium falciparum
5-Methylorotate
-
Plasmodium falciparum
atovaquone
-
Plasmodium falciparum
benzoquinones ubiquinone-0 and decylubiquinone Plasmodium falciparum
chloronaphthoquinone
-
Plasmodium falciparum
Lapachol
-
Plasmodium falciparum
Lawsone
-
Plasmodium falciparum
menadione
-
Plasmodium falciparum
Orotate also inhibitors: 5-substituted orotates; competitive to dihydroorotate; strong competitive inhibitor Plasmodium falciparum
rhodamine 123 mitochondrial inhibitor Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
coenzyme Q6
-
Plasmodium falciparum
0.014
-
dihydroorotate
-
Plasmodium falciparum
0.0225
-
ubiquinone-6
-
Plasmodium falciparum
0.0144
-
S-dihydroorotate
-
Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Plasmodium falciparum
-
-
mitochondrion inner membrane Plasmodium falciparum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
gel filtration Plasmodium falciparum
56000
-
gel filtration, SDS-PAGE Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-dihydroorotate + ubiquinone Plasmodium falciparum fourth step in synthesis of uridine 5'-monophosphate orotate + ubiquinol
-
?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-
Plasmodium falciparum
-
mature trophozytes and schizonts
-

Purification (Commentary)

Purification (Comment) Organism
-
Plasmodium falciparum
cation-, anion-exchange, gel filtration Plasmodium falciparum

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0002672
-
37°C Plasmodium falciparum

Storage Stability

Storage Stability Organism
4°C, extremely labile, 90% decrease of activity over night Plasmodium falciparum
-80°C, addition of S-dihydroorotate to stabilize the enzyme activity Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + ubiquinone ubiquinone-6, ubiquinone-7, ubiquinone-9 and ubiquinone-10 maximize enzyme activity Plasmodium falciparum orotate + ubiquinol
-
?
(S)-dihydroorotate + ubiquinone fourth step in synthesis of uridine 5'-monophosphate Plasmodium falciparum orotate + ubiquinol
-
?
dihydroorotate + acceptor acceptor: quinones Plasmodium falciparum orotate + reduced acceptor
-
?
L-dihydroorotate + ubiquinone-6
-
Plasmodium falciparum orotate + reduced ubiquinone-6
-
?

Subunits

Subunits Comment Organism
monomer 1 * 56000, SDS-PAGE Plasmodium falciparum
monomer SDS-PAGE Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.36
-
ubiquinone6
-
Plasmodium falciparum
0.25
-
S-dihydroorotate
-
Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.018
-
Orotate
-
Plasmodium falciparum
0.0182
-
Orotate
-
Plasmodium falciparum
0.0151
-
5-Fluoroorotate
-
Plasmodium falciparum
0.0244
-
5-aminoorotate
-
Plasmodium falciparum
0.1185
-
5-Methylorotate
-
Plasmodium falciparum
0.1408
-
5-bromoorotate
-
Plasmodium falciparum
0.2246
-
5-iodoorotate
-
Plasmodium falciparum