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Literature summary for 1.4.1.8 extracted from

  • Hyun, C.G.; Kim, S.S.; Park, K.H.; Suh, J.W.
    Valine dehydrogenase from Streptomyces albus: gene cloning, heterologous expression and identification of active site by site-directed mutagenesis (2000), FEMS Microbiol. Lett., 182, 29-34.
    View publication on PubMed
Search for more literature for 1.4.1.8

Cloned(Commentary)

Cloned (Comment) Organism
valDH gene cloned to identify the active site, coding region expressed in Escherichia coli using a pET expression system Streptomyces albus
vdh gene Streptomyces coelicolor
vdh gene Streptomyces fradiae
vdh gene Streptomyces virginiae
vdh gene Kitasatospora aureofaciens

Protein Variants

Protein Variants Comment Organism
K79A lower affinity for L-valine and 2-oxoisovalerate and lower catalytic rate constants compared to the wild type enzyme Streptomyces albus
K91A lower affinity for L-valine and 2-oxoisovalerate and lower catalytic rate constants compared to the wild type enzyme Streptomyces albus
additional information active site lysine residue modified with pyridoxal 5’-phosphate Streptomyces albus

Inhibitors

Inhibitors Comment Organism Structure
pyridoxal 5'-phosphate 40% loss of activity, forms Schiff base with a lysine residue Streptomyces albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 NADH K91A mutant Streptomyces albus
0.029
-
 NADH K79A mutant Streptomyces albus
0.0576
-
 NADH
-
 Streptomyces albus
0.13
-
 NAD+
-
 Streptomyces albus
0.19
-
 NAD+ K91A mutant Streptomyces albus
0.2
-
 NAD+ K79A mutant Streptomyces albus
0.438
-
 2-oxoisovalerate
-
 Streptomyces albus
1.5
-
 L-valine
-
 Streptomyces albus
2.08
-
 L-valine K91A mutant Streptomyces albus
4.5
-
 2-oxoisovalerate K91A mutant Streptomyces albus
50
-
 2-oxoisovalerate K79A mutant Streptomyces albus
714
-
 L-valine K79A mutant Streptomyces albus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble soluble enzyme expressed in Escherichia coli Streptomyces albus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, SDS-PAGE Streptomyces albus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-valine + NAD(P)+ + H2O Streptomyces virginiae
-
 2-oxoisovalerate + NH3 + NAD(P)H
-
 ?
L-valine + NAD(P)+ + H2O Streptomyces coelicolor metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics 2-oxoisovalerate + NH3 + NAD(P)H
-
 ?
L-valine + NAD(P)+ + H2O Streptomyces albus metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics 2-oxoisovalerate + NH3 + NAD(P)H
-
 ?
L-valine + NAD(P)+ + H2O Streptomyces fradiae metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics 2-oxoisovalerate + NH3 + NAD(P)H
-
 ?
L-valine + NAD(P)+ + H2O Kitasatospora aureofaciens metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics 2-oxoisovalerate + NH3 + NAD(P)H
-
 ?
L-valine + NAD(P)+ + H2O Streptomyces albus first catabolic step of L-valine and some other branched chain L-amino acids, important role in providing precursors for biosynthesis of polyether antibiotic, salinomycin, that is produced at a high level 2-oxoisovalerate + NH3 + NAD(P)H
-
 ?

Organism

Organism UniProt Comment Textmining
Kitasatospora aureofaciens
-
 spiramycin producer
-
Streptomyces albus
-
 salinomycin producer
-
Streptomyces albus
-
 strain ATCC 21838  
-
Streptomyces coelicolor
-
 actinorhodin producer
-
Streptomyces fradiae
-
 tylosin producer
-
Streptomyces virginiae
-
 monensin producer
-

Purification (Commentary)

Purification (Comment) Organism
His-bind resin column, 95% purity Streptomyces albus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00534
-
 L-phenylalanine Streptomyces albus
1.056
-
 L-cysteine Streptomyces albus
4.2
-
 L-leucine Streptomyces albus
7.02
-
 L-isoleucine Streptomyces albus
7.68
-
 L-alanine Streptomyces albus
52.01
-
 2-aminobutyrate Streptomyces albus
56.33
-
 L-valine Streptomyces albus
81.8
-
 2-oxoisocaproate Streptomyces albus
107.6
-
 2-oxo-3-methylvalerate Streptomyces albus
278
-
 2-oxobutyrate Streptomyces albus
302.4
-
 2-oxoisovalerate Streptomyces albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutyrate + H2O + NAD+ preferred substrate, 92.34% of the activity with L-valine Streptomyces albus 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate r
L-2-aminobutyrate + H2O + NAD+ preferred substrate, 92.34% of the activity with L-valine Streptomyces albus 2-oxobutyrate + NADH + NH3 reductive amination: 91.8% of the activity with 2-oxoisovalerate r
L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Streptomyces coelicolor 2-oxobutyrate + NADH + NH3
-
 ?
L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Streptomyces albus 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate r
L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Streptomyces fradiae 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate r
L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Streptomyces virginiae 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate r
L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Kitasatospora aureofaciens 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate r
L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Streptomyces albus 2-oxobutyrate + NADH + NH3 reductive amination: 91.8% of the activity with 2-oxoisovalerate r
L-alanine + H2O + NAD+
-
 Streptomyces coelicolor pyruvate + NH3 + NADH
-
 r
L-alanine + H2O + NAD+
-
 Streptomyces fradiae pyruvate + NH3 + NADH
-
 r
L-alanine + H2O + NAD+
-
 Streptomyces virginiae pyruvate + NH3 + NADH
-
 ?
L-alanine + H2O + NAD+
-
 Kitasatospora aureofaciens pyruvate + NH3 + NADH
-
 r
L-alanine + H2O + NAD+ 13.63% of the activity with L-valine Streptomyces albus pyruvate + NH3 + NADH
-
 r
L-cysteine + H2O + NAD+
-
 Streptomyces coelicolor 3-mercapto-2-oxopropanoate + NH3 + NADH
-
 ?
L-cysteine + H2O + NAD+ 1.87% of the activity with L-valine Streptomyces albus 3-mercapto-2-oxopropanoate + NH3 + NADH
-
 r
L-isoleucine + H2O + NAD+
-
 Streptomyces coelicolor 3-methyl-2-oxopentanoate + NADH + NH3
-
 r
L-isoleucine + H2O + NAD+
-
 Streptomyces virginiae 3-methyl-2-oxopentanoate + NADH + NH3
-
 r
L-isoleucine + H2O + NAD+
-
 Streptomyces fradiae 3-methyl-2-oxopentanoate + NADH + NH3 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate r
L-isoleucine + H2O + NAD+
-
 Kitasatospora aureofaciens 3-methyl-2-oxopentanoate + NADH + NH3 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate r
L-isoleucine + H2O + NAD+ 12.46% of the activity with L-valine Streptomyces albus 3-methyl-2-oxopentanoate + NADH + NH3 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate r
L-isoleucine + H2O + NAD+ 12.46% of the activity with L-valine Streptomyces albus 3-methyl-2-oxopentanoate + NADH + NH3 reductive amination: 35.6% of the activity with 2-oxoisovalerate r
L-leucine + H2O + NAD+
-
 Streptomyces coelicolor 2-oxoisocaproate + NADH + NH3
-
 ?
L-leucine + H2O + NAD+
-
 Streptomyces fradiae 2-oxoisocaproate + NADH + NH3 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate r
L-leucine + H2O + NAD+
-
 Streptomyces virginiae 2-oxoisocaproate + NADH + NH3 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate r
L-leucine + H2O + NAD+
-
 Kitasatospora aureofaciens 2-oxoisocaproate + NADH + NH3 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate r
L-leucine + H2O + NAD+ 7.46% of the activity with L-valine Streptomyces albus 2-oxoisocaproate + NADH + NH3 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate r
L-leucine + H2O + NAD+ 7.46% of the activity with L-valine Streptomyces albus 2-oxoisocaproate + NADH + NH3 reductive amination: 26.85% of the activity with 2-oxoisovalerate r
L-phenylalanine + H2O + NAD+ only 0.95% of the activity with L-valine Streptomyces albus 2-oxo-3-phenylpropanoic acid + NH3 + NADH
-
 r
L-valine + NAD(P)+ + H2O
-
 Streptomyces virginiae 2-oxoisovalerate + NH3 + NAD(P)H
-
 ?
L-valine + NAD(P)+ + H2O preferred substrate Streptomyces albus 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O preferred substrate Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O preferred substrate Streptomyces albus 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
L-valine + NAD(P)+ + H2O preferred substrate Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
L-valine + NAD(P)+ + H2O preferred substrate Streptomyces albus 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
L-valine + NAD(P)+ + H2O preferred substrate Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces albus 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces virginiae 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces albus 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces virginiae 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces albus 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces virginiae 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
L-valine + NAD(P)+ + H2O metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H
-
 ?
L-valine + NAD(P)+ + H2O metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics Streptomyces albus 2-oxoisovalerate + NH3 + NAD(P)H
-
 ?
L-valine + NAD(P)+ + H2O metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H
-
 ?
L-valine + NAD(P)+ + H2O metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics Kitasatospora aureofaciens 2-oxoisovalerate + NH3 + NAD(P)H
-
 ?
L-valine + NAD(P)+ + H2O first catabolic step of L-valine and some other branched chain L-amino acids, important role in providing precursors for biosynthesis of polyether antibiotic, salinomycin, that is produced at a high level Streptomyces albus 2-oxoisovalerate + NH3 + NAD(P)H
-
 ?
additional information
-
 Streptomyces fradiae additional information
-
 ?
additional information
-
 Streptomyces virginiae additional information
-
 ?
additional information Lys-79, Lys-91 are involved in substrate binding and catalysis Streptomyces albus additional information
-
 ?
additional information not: D-valine Streptomyces coelicolor additional information
-
 ?
additional information not: D-valine Streptomyces albus additional information
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Streptomyces albus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000483
-
 L-valine K79A mutant Streptomyces albus
0.0252
-
 L-valine K91A mutant Streptomyces albus
7.22
-
 2-oxoisovalerate K79A mutant Streptomyces albus
13
-
 2-oxoisovalerate K91A mutant Streptomyces albus
47.5
-
 L-valine
-
 Streptomyces albus
315
-
 2-oxoisovalerate
-
 Streptomyces albus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Streptomyces coelicolor
NAD+
-
 Streptomyces albus
NAD+
-
 Streptomyces fradiae
NAD+
-
 Streptomyces virginiae
NAD+
-
 Kitasatospora aureofaciens
NADH
-
 Streptomyces coelicolor
NADH
-
 Streptomyces albus
NADH
-
 Streptomyces fradiae
NADH
-
 Streptomyces virginiae
NADH
-
 Kitasatospora aureofaciens