Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Origami2(DE3) cells | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
histamine | substrate inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
histamine | in 100 mM potassium phosphate, pH 7.2, at 37°C | Rattus norvegicus | |
0.45 | - |
tyramine | in 100 mM potassium phosphate, pH 7.2, at 37°C | Rattus norvegicus | |
0.64 | - |
benzylamine | in 100 mM potassium phosphate, pH 7.2, at 37°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | Cu2+ ions stimulate the activation/maturation of the enzyme. The highest activity is observed at more than 5fold molar ratio between Cu2+ and the enzyme | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
histamine + H2O + O2 | Rattus norvegicus | preferred substrate | (imidazol-4-yl)acetaldehyde + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylamine + H2O + O2 | - |
Rattus norvegicus | benzaldehyde + NH3 + H2O2 | - |
? | |
histamine + H2O + O2 | preferred substrate | Rattus norvegicus | (imidazol-4-yl)acetaldehyde + NH3 + H2O2 | - |
? | |
phenylethylamine + H2O + O2 | - |
Rattus norvegicus | ? | - |
? | |
tyramine + H2O + O2 | - |
Rattus norvegicus | (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
DAO | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
histamine | pH and temperature not specified in the publication | Rattus norvegicus |