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Literature summary for 1.4.3.22 extracted from

  • Gludovacz, E.; Maresch, D.; Lopes de Carvalho, L.; Puxbaum, V.; Baier, L.J.; Suetzl, L.; Guedez, G.; Gruenwald-Gruber, C.; Ulm, B.; Pils, S.; Ristl, R.; Altmann, F.; Jilma, B.; Salminen, T.A.; Borth, N.; Boehm, T.
    Oligomannosidic glycans at Asn-110 are essential for secretion of human diamine oxidase (2018), J. Biol. Chem., 293, 1070-1087 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in two different HEK293 cell lines Homo sapiens

Protein Variants

Protein Variants Comment Organism
N168Q/N538Q/N745Q the secretion of the recombinant enzyme is reduced by 94% Homo sapiens
N538Q/N745Q the secretion of the recombinant enzyme is reduced by 85% Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P19801
-
-
Sus scrofa Q9TRC7
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Sus scrofa
glycoprotein the enzyme has multiple N-glycosylation sites. The N-glycosylation sites Asn168, Asn538, and Asn745 in recombinant human diamine oxidase carry complex-type glycans, whereas Asn110 carries only mammalian-atypical oligomannosidic glycans. Glycans at Asn168 are predominantly sialylated with bi- to tetra-antennary branches, and Asn538 and Asn745 have similar complex-type glycans with some tissue- and cell line-specific variations. Mutations of Asn168, Asn538, and Asn745 reduce secretion of the recombinant enzyme by 13, 71, and 32%, respectively. Asn538/745 double and Asn168/538/745 triple substitutions reduce rhDAO secretion by 85% and 94%. Because of their locations in the diamine oxidase structure, Asn538 and Asn745 glycosylations might be important for efficient DAO dimer formation. Glycosylation seems essential for reaching high enzyme expression levels in the gastrointestinal tract, kidney, and placenta Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
amniotic fluid
-
 Homo sapiens
-
Caco-2 cell
-
 Homo sapiens
-
gastrointestinal tract
-
 Homo sapiens
-
kidney
-
 Sus scrofa
-
kidney
-
 Homo sapiens
-
placenta
-
 Homo sapiens
-

Synonyms

Synonyms Comment Organism
DAO
-
 Sus scrofa
DAO
-
 Homo sapiens

General Information

General Information Comment Organism
evolution high evolutionary conservation of all four glycosylation sites Homo sapiens
metabolism the enzyme is involved in histamine metabolism Sus scrofa
metabolism the enzyme is involved in histamine metabolism Homo sapiens