BRENDA - Enzyme Database show
show all sequences of 1.5.4.1

Purification and properties of the enzymes from Drosophila melanogaster that catalyze the conversion of dihydroneopterin triphosphate to the pyrimidodiazepine precursor of the drosopterins

Wiederrecht, G.J.; Brown, G.M.; J. Biol. Chem. 259, 14121-14127 (1984)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0085
-
dihydroneopterin triphosphate
-
Drosophila melanogaster
1.8
-
glutathione
-
Drosophila melanogaster
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
2 * 24000, SDS-PAGE
Drosophila melanogaster
48000
-
gel filtration
Drosophila melanogaster
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-pyruvoyltetrahydropterin + glutathione
Drosophila melanogaster
enzyme is involved in the formation of the eye pigment drosopterin in Drosophila melanogaster, eye color mutant sepia is missing PDA synthase
glutathione disulfide + pyrimidodiazepine
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Drosophila melanogaster
-
wild-type flies
-
Purification (Commentary)
Commentary
Organism
-
Drosophila melanogaster
Source Tissue
Source Tissue
Commentary
Organism
Textmining
head
-
Drosophila melanogaster
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.8
-
-
Drosophila melanogaster
Storage Stability
Storage Stability
Organism
-80°C, enzyme purified through the Affi-Gel blue step is stable for at least 9 months
Drosophila melanogaster
-80°C, enzyme purified through the final step stable for at least 1-2 weeks
Drosophila melanogaster
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-pyruvoyltetrahydropterin + glutathione
-
437751
Drosophila melanogaster
glutathione disulfide + pyrimidodiazepine
-
-
-
r
6-pyruvoyltetrahydropterin + glutathione
enzyme is involved in the formation of the eye pigment drosopterin in Drosophila melanogaster, eye color mutant sepia is missing PDA synthase
437751
Drosophila melanogaster
glutathione disulfide + pyrimidodiazepine
-
-
-
r
dihydroneopterin triphosphate + glutathione
-
437751
Drosophila melanogaster
?
-
-
-
-
glutathione disulfide + pyrimidodiazepine
-
437751
Drosophila melanogaster
6-pyruvoyltetrahydropterin + glutathione
-
-
-
r
pyruvoyltetrahydropterin + glutathione
-
437751
Drosophila melanogaster
2-amino-4-oxo-6-acetyl-7,8-dihydro-3H,9H-pyrimido[4,5-b]-[1,4]diazepine
PDA
437751
Drosophila melanogaster
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 24000, SDS-PAGE
Drosophila melanogaster
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Drosophila melanogaster
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0085
-
dihydroneopterin triphosphate
-
Drosophila melanogaster
1.8
-
glutathione
-
Drosophila melanogaster
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
2 * 24000, SDS-PAGE
Drosophila melanogaster
48000
-
gel filtration
Drosophila melanogaster
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-pyruvoyltetrahydropterin + glutathione
Drosophila melanogaster
enzyme is involved in the formation of the eye pigment drosopterin in Drosophila melanogaster, eye color mutant sepia is missing PDA synthase
glutathione disulfide + pyrimidodiazepine
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Drosophila melanogaster
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
head
-
Drosophila melanogaster
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.8
-
-
Drosophila melanogaster
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, enzyme purified through the Affi-Gel blue step is stable for at least 9 months
Drosophila melanogaster
-80°C, enzyme purified through the final step stable for at least 1-2 weeks
Drosophila melanogaster
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-pyruvoyltetrahydropterin + glutathione
-
437751
Drosophila melanogaster
glutathione disulfide + pyrimidodiazepine
-
-
-
r
6-pyruvoyltetrahydropterin + glutathione
enzyme is involved in the formation of the eye pigment drosopterin in Drosophila melanogaster, eye color mutant sepia is missing PDA synthase
437751
Drosophila melanogaster
glutathione disulfide + pyrimidodiazepine
-
-
-
r
dihydroneopterin triphosphate + glutathione
-
437751
Drosophila melanogaster
?
-
-
-
-
glutathione disulfide + pyrimidodiazepine
-
437751
Drosophila melanogaster
6-pyruvoyltetrahydropterin + glutathione
-
-
-
r
pyruvoyltetrahydropterin + glutathione
-
437751
Drosophila melanogaster
2-amino-4-oxo-6-acetyl-7,8-dihydro-3H,9H-pyrimido[4,5-b]-[1,4]diazepine
PDA
437751
Drosophila melanogaster
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 24000, SDS-PAGE
Drosophila melanogaster
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Drosophila melanogaster
Other publictions for EC 1.5.4.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
671875
Kim
Identification and characteris ...
Drosophila melanogaster
Biochem. J.
398
451-460
2006
-
-
1
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
3
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
3
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656799
Giordano
The clot gene of Drosophila me ...
Drosophila melanogaster
Mol. Genet. Genomics
268
692-697
2003
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437751
Wiederrecht
Purification and properties of ...
Drosophila melanogaster
J. Biol. Chem.
259
14121-14127
1984
-
-
-
-
-
-
-
2
-
-
2
1
-
4
-
-
1
-
-
1
1
2
5
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
1
1
2
5
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-