Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methylophilus methylotrophus |
Protein Variants | Comment | Organism |
---|---|---|
H172Q | no change of turnover, but 25fold increase of Km-value | Methylophilus methylotrophus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
trimethylamine | - |
Methylophilus methylotrophus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine + H2O + electron transferring flavoprotein | Methylophilus methylotrophus | - |
dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? | |
trimethylamine + H2O + electron transferring flavoprotein | Methylophilus methylotrophus W3A1 | - |
dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophilus methylotrophus | - |
- |
- |
Methylophilus methylotrophus W3A1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, recombinant enzyme | Methylophilus methylotrophus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Methylophilus methylotrophus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine + H2O + electron transferring flavoprotein | - |
Methylophilus methylotrophus | dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? | |
trimethylamine + H2O + electron transferring flavoprotein | - |
Methylophilus methylotrophus W3A1 | dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Methylophilus methylotrophus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
trimethylamine | - |
Methylophilus methylotrophus |