Any feedback?
Literature summary for 1.5.99.4 extracted from
- Nicotine dehydrogenase from Arthrobacter oxidans: A molybdenum-containing hydroxylase (1988), FEMS Microbiol. Lett., 52, 13-18.
No PubMed abstract available
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | four iron groups per mol | Pseudarthrobacter oxydans |  |
| Molybdenum | one molecule per mol | Pseudarthrobacter oxydans | |
| additional information | two labile sulfur groups per mol | Pseudarthrobacter oxydans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15100 | - |
1 * 82000 + 1 * 30000 + 1 * 15100, SDS-PAGE | Pseudarthrobacter oxydans |
| 30000 | - |
1 * 82000 + 1 * 30000 + 1 * 15100, SDS-PAGE | Pseudarthrobacter oxydans |
| 82000 | - |
1 * 82000 + 1 * 30000 + 1 * 15100, SDS-PAGE | Pseudarthrobacter oxydans |
| 120000 | - |
gel filtration | Pseudarthrobacter oxydans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotine + acceptor + H2O | Pseudarthrobacter oxydans | first enzyme in the nicotine degradation pathway | 6-hydroxynicotine + reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudarthrobacter oxydans | - |
P-34 DSM 419 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, ion-exchange, gel filtration | Pseudarthrobacter oxydans |
| hydroxylapatite column | Pseudarthrobacter oxydans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.3 | - |
- |
Pseudarthrobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotine + acceptor + H2O | preferentially 2,6-dichlorophenolindophenol used as electron acceptor | Pseudarthrobacter oxydans | 6-hydroxynicotine + reduced acceptor | - |
? | |
| nicotine + acceptor + H2O | molybdate is required | Pseudarthrobacter oxydans | 6-hydroxynicotine + reduced acceptor | - |
? | |
| nicotine + acceptor + H2O | first enzyme in the nicotine degradation pathway | Pseudarthrobacter oxydans | 6-hydroxynicotine + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 1 * 82000 + 1 * 30000 + 1 * 15100, SDS-PAGE | Pseudarthrobacter oxydans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pseudarthrobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Pseudarthrobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | one mol per mol | Pseudarthrobacter oxydans | |
| FAD | not covalently bound | Pseudarthrobacter oxydans | |
| additional information | molybdo-iron-sulfur-flavoprotein | Pseudarthrobacter oxydans | |
| pterin | part of molybdenum cofactor | Pseudarthrobacter oxydans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
