BRENDA - Enzyme Database
show all sequences of 1.5.99.4

Rabbit nasal cytochrome P-450 NMa has high activity as a nicotine oxidase

Williams, D.E.; Ding, X.; Coon, M.J.; Biochem. Biophys. Res. Commun. 166, 945-952 (1990)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
(S)-nicotine
-
Oryctolagus cuniculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
olfactory and respiratory
Oryctolagus cuniculus
-
-
Organism
Organism
UniProt
Commentary
Textmining
Oryctolagus cuniculus
-
male, adult, New Zealand White
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
nose
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0065
-
respiratory microsomes
Oryctolagus cuniculus
0.0222
-
olfactory microsomes
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(S)-nicotine + acceptor + H2O
-
392540
Oryctolagus cuniculus
nornicotine + reduced acceptor
-
392540
Oryctolagus cuniculus
?
(S)-nicotine + acceptor + H2O
-
392540
Oryctolagus cuniculus
nicotine-N-oxide + reduced acceptor
-
392540
Oryctolagus cuniculus
?
(S)-nicotine + acceptor + H2O
-
392540
Oryctolagus cuniculus
(S)-nicotine delta1',5'-iminium ion + reduced acceptor
-
392540
Oryctolagus cuniculus
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Oryctolagus cuniculus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.468
-
(S)-nicotine
respiratory
Oryctolagus cuniculus
0.597
-
(S)-nicotine
olfactory
Oryctolagus cuniculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Oryctolagus cuniculus
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
P-450 NMa monooxygenase
Oryctolagus cuniculus
additional information
flavoprotein
Oryctolagus cuniculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
P-450 NMa monooxygenase
Oryctolagus cuniculus
additional information
flavoprotein
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
(S)-nicotine
-
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
olfactory and respiratory
Oryctolagus cuniculus
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
nose
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0065
-
respiratory microsomes
Oryctolagus cuniculus
0.0222
-
olfactory microsomes
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(S)-nicotine + acceptor + H2O
-
392540
Oryctolagus cuniculus
nornicotine + reduced acceptor
-
392540
Oryctolagus cuniculus
?
(S)-nicotine + acceptor + H2O
-
392540
Oryctolagus cuniculus
nicotine-N-oxide + reduced acceptor
-
392540
Oryctolagus cuniculus
?
(S)-nicotine + acceptor + H2O
-
392540
Oryctolagus cuniculus
(S)-nicotine delta1',5'-iminium ion + reduced acceptor
-
392540
Oryctolagus cuniculus
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Oryctolagus cuniculus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.468
-
(S)-nicotine
respiratory
Oryctolagus cuniculus
0.597
-
(S)-nicotine
olfactory
Oryctolagus cuniculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Oryctolagus cuniculus
Other publictions for EC 1.5.99.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741698
Li
Nicotine dehydrogenase comple ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens S33
Appl. Environ. Microbiol.
82
1745-1755
2016
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1
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7
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1
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2
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1
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1
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1
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1
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-
741709
Qiu
Conversion of nornicotine to ...
Shinella sp. HZN7
Appl. Microbiol. Biotechnol.
100
10019-10029
2016
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2
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4
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2
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1
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2
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724032
Qiu
Cloning of a novel nicotine ox ...
no activity in Escherichia coli strain DH5alpha, no activity in Pseudomonas putida strain KT2440, Pseudomonas sp., Pseudomonas sp. HZN6
Appl. Environ. Microbiol.
79
2164-2171
2013
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1
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2
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2
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4
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1
1
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2
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4
-
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-
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-
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-
4
4
-
-
-
684760
Ganas
Two closely related pathways o ...
Nocardioides sp., Paenarthrobacter nicotinovorans
Arch. Microbiol.
189
511-517
2008
-
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2
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4
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3
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4
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4
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3
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671467
Sachelaru
A functional mobA gene for mol ...
Paenarthrobacter nicotinovorans
Appl. Environ. Microbiol.
72
5126-5131
2006
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-
-
-
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1
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1
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1
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1
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655850
Igloi
Sequence of the 165-kilobase c ...
Paenarthrobacter nicotinovorans
J. Bacteriol.
185
1976-1986
2003
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-
1
-
-
-
-
1
-
-
-
2
-
3
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1
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2
-
1
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1
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1
1
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1
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2
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1
-
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2
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-
656187
Sandu
Characterization of HdnoR, the ...
Paenarthrobacter nicotinovorans
J. Biol. Chem.
278
51307-51315
2003
-
-
1
-
-
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1
-
-
-
-
3
-
3
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1
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3
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2
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1
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1
1
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1
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3
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1
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-
3
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-
392430
Schenk
Gene structures and properties ...
Paenarthrobacter nicotinovorans
J. Mol. Biol.
284
1323-1339
1998
-
-
-
-
-
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-
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2
1
1
-
4
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1
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1
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1
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2
1
1
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1
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-
-
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-
392431
Grether-Beck
Structural analysis and molybd ...
Paenarthrobacter nicotinovorans
Mol. Microbiol.
13
929-936
1994
-
-
1
-
-
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1
-
2
2
3
-
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2
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1
1
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2
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1
2
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1
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2
2
3
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1
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-
-
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-
1
-
-
1
-
-
392540
Williams
Rabbit nasal cytochrome P-450 ...
Oryctolagus cuniculus
Biochem. Biophys. Res. Commun.
166
945-952
1990
-
-
-
-
-
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-
1
1
-
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-
1
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1
2
-
3
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1
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2
1
-
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2
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2
-
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-
1
1
-
-
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-
1
2
-
3
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
391423
Nagel
-
Bactopterin as component of eu ...
Pseudarthrobacter oxydans
FEMS Microbiol. Lett.
60
323-326
1989
-
-
-
-
-
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3
-
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1
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1
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1
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1
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-
-
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392537
Freudenberg
-
Nicotine dehydrogenase from Ar ...
Pseudarthrobacter oxydans
FEMS Microbiol. Lett.
52
13-18
1988
-
-
-
-
-
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-
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3
4
1
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1
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1
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1
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3
1
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1
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1
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3
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3
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3
4
1
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1
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1
-
3
1
1
-
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1
-
-
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-
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-
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-
392541
Nakayama
Heterogeneity of hepatic nicot ...
Rattus norvegicus
Biochim. Biophys. Acta
715
254-257
1982
-
-
-
-
-
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1
-
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1
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1
1
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1
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1
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1
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2
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2
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1
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1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
392542
Jones
Cytochrome c linked nicotinic ...
Pseudomonas putida, Pseudomonas putida Chester
FEBS Lett.
32
321-324
1973
1
-
-
-
-
-
5
-
-
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5
-
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1
1
2
-
1
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1
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1
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1
-
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5
-
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1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
392538
Hochstein
The purification and propertie ...
Pseudarthrobacter oxydans
Biochim. Biophys. Acta
139
56-68
1967
-
-
-
-
-
-
11
6
-
1
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1
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1
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1
3
6
-
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1
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3
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3
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3
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11
-
6
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1
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1
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1
3
6
-
1
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3
-
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-
-
-
-
392440
Decker
Induction and purification of ...
Pseudarthrobacter oxydans
Biochim. Biophys. Acta
105
313-324
1965
-
-
-
-
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1
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1
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1
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1
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1
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2
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1
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1
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392543
Behrman
The bacterial oxidation of nic ...
Pseudomonas fluorescens, Pseudomonas fluorescens N-9
J. Biol. Chem.
228
923-945
1957
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1
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1
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1
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1
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