Cloned (Comment) | Organism |
---|---|
recombinantly expressed in Escherichia coli | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(12aS,12bS)-7-fluoro-2,3,5,12,12a,12b-hexahydro-1H,4H-11-oxa-3a,9b-diazabenzo[a]naphtho[2,1,8-cde]azulene | - |
Escherichia coli | |
(1R,7aR)-hexahydro-1H-pyrrolizin-1-ylmethyl 4-hydroxy-3,5-dimethoxybenzoate | - |
Escherichia coli | |
1,2,5-trimethyl-2,3,4,6-tetrahydro-1H-pyrido[4,3-b]carbazole | - |
Escherichia coli | |
2,5,11-trimethyl-2,3,4,6-tetrahydro-1H-pyrido[4,3-b]carbazole | - |
Escherichia coli | |
3beta,5alpha-17a-aza-D-homoandrostan-3-ol | - |
Escherichia coli | |
5'-S-[2-(decylamino)ethyl]-5'-thioadenosine | this compound is also found to completely inhibit cyclopropanation of the phospholipids in growing Escherichia coli cells in vivo, at 0.15 mM | Escherichia coli | |
9-methoxy-1,2,5-trimethyl-2,3,4,6-tetrahydro-1H-pyrido[4,3-b]carbazole | - |
Escherichia coli | |
dioctylamine | - |
Escherichia coli | |
sinefungin | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km (phospholipids): 0.5 mg/ml. Since a mixture of phospholipids prepared from an Escherichia coli K12 strain is used, the Km constant is only an apparent constant given in mg/mL unit rather than molar unit | Escherichia coli | |
0.08 | - |
S-adenosyl-L-methionine | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + phospholipids | - |
Escherichia coli | S-adenosyl-L-homocysteine + phospholipid + cyclopropane fatty acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CFAS | - |
Escherichia coli |
cyclopropane fatty acid synthase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00013 | - |
dioctylamine | competitive inhibitor with respect to phospholipids | Escherichia coli | |
0.00026 | - |
dioctylamine | uncompetitive inhibitor with respect to S-adenosyl-L-methionine | Escherichia coli | |
0.0005 | - |
sinefungin | competitive inhibitor with respect to S-adenosyl-L-methionine | Escherichia coli | |
0.0011 | - |
(12aS,12bS)-7-fluoro-2,3,5,12,12a,12b-hexahydro-1H,4H-11-oxa-3a,9b-diazabenzo[a]naphtho[2,1,8-cde]azulene | inhibits CAFS in a mixed-type fashion with respect to phospholipids | Escherichia coli | |
0.0014 | - |
(12aS,12bS)-7-fluoro-2,3,5,12,12a,12b-hexahydro-1H,4H-11-oxa-3a,9b-diazabenzo[a]naphtho[2,1,8-cde]azulene | inhibits the CFAS non-competitively with respect to AdoMet | Escherichia coli | |
0.006 | - |
5'-S-[2-(decylamino)ethyl]-5'-thioadenosine | apparent inhibition constant when the phospholipids are the variable substrates. Noncompetitive inhibitor with respect to both substrates | Escherichia coli | |
0.0072 | - |
sinefungin | non-competitive inhibitor with respect to the phospholipids | Escherichia coli | |
0.016 | - |
5'-S-[2-(decylamino)ethyl]-5'-thioadenosine | apparent inhibition constant when S-adenosyl-L-methionine is the variable substrates. Noncompetitive inhibitor with respect to both substrates | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.001 | - |
- |
Escherichia coli | 9-methoxy-1,2,5-trimethyl-2,3,4,6-tetrahydro-1H-pyrido[4,3-b]carbazole | |
0.004 | - |
- |
Escherichia coli | dioctylamine | |
0.004 | - |
- |
Escherichia coli | 1,2,5-trimethyl-2,3,4,6-tetrahydro-1H-pyrido[4,3-b]carbazole | |
0.005 | - |
- |
Escherichia coli | 2,5,11-trimethyl-2,3,4,6-tetrahydro-1H-pyrido[4,3-b]carbazole | |
0.007 | - |
- |
Escherichia coli | (12aS,12bS)-7-fluoro-2,3,5,12,12a,12b-hexahydro-1H,4H-11-oxa-3a,9b-diazabenzo[a]naphtho[2,1,8-cde]azulene | |
0.009 | - |
- |
Escherichia coli | sinefungin | |
0.009 | - |
- |
Escherichia coli | 3beta,5alpha-17a-aza-D-homoandrostan-3-ol | |
0.01 | - |
- |
Escherichia coli | 5'-S-[2-(decylamino)ethyl]-5'-thioadenosine | |
0.01 | - |
- |
Escherichia coli | (1R,7aR)-hexahydro-1H-pyrrolizin-1-ylmethyl 4-hydroxy-3,5-dimethoxybenzoate |