Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and mutant enzymes in Corynebacterium glutamicum strain ATCC 13032, subcloning in Escherichia coli strain DH5alpha | Streptomyces mobaraensis |
Protein Variants | Comment | Organism |
---|---|---|
E164L | site-directed mutagenesis, the E164L mutant exhibits a 1.95fold increased specific activity and 1.66fold increased half-life at 50°C compared to wild-type. The molecular dynamics (MD) simulation results indicate that the mutation Glu164Leu results in weaker interactions of Asp159-Glu164 and Gly228-Leu231, leading to the enhanced instability of Ile240-Asn253 linked to Gly228-Leu231 by eight residues. It further causes reduced interactions between loop region 1 (Ile240-Asn253) and loop region 2 (His277-Met288), facilitating the access of substrate molecule to the active site. Structure-activity relationship for MTG adapted to high temperature conditions. Enhancing activity and thermostability of Streptomyces mobaraensis transglutaminase by directed evolution, Molecular mechanism of improved activity of E164L analyzed by molecular dynamics simulations | Streptomyces mobaraensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein glutamine + alkylamine | Streptomyces mobaraensis | - |
protein N5-alkylglutamine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces mobaraensis | P81453 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-CBZ-Glu-Gly + hydroxylamine | - |
Streptomyces mobaraensis | CBZ-Glu-(gamma-monohydroxamate)-Gly + NH3 | - |
? | |
protein glutamine + alkylamine | - |
Streptomyces mobaraensis | protein N5-alkylglutamine + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MTG | - |
Streptomyces mobaraensis |
transglutaminase | - |
Streptomyces mobaraensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Streptomyces mobaraensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | activity range, wild-type and mutant enzyme, profiles overview | Streptomyces mobaraensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
after treatment at 50°C and pH 7.0 for 60, 80, 100, and 120 min, respectively, recombinant mutant E164L maintains 60%, 41%, 29%, and 16% of its original activity | Streptomyces mobaraensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
recombinant enzyme | Streptomyces mobaraensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | activity range, wild-type and mutant enzyme, profiles overview | Streptomyces mobaraensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
after treatment at 50°C and pH 7.0 for 60, 80, 100, and 120 min, respectively, recombinant mutant E164L maintains 60%, 41%, 29%, and 16% of its original activity | Streptomyces mobaraensis |
General Information | Comment | Organism |
---|---|---|
additional information | molecular dynamics simulations | Streptomyces mobaraensis |