Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein in Escherichia coli | Pseudomonas aeruginosa |
residues 142-579 are expressed as a His-tagged fusion protein in Escherichia coli | Vibrio cholerae |
Protein Variants | Comment | Organism |
---|---|---|
D484E | RXXD allosteric inhibition site of the protein is mutated (VC2370(142)-D484E) because it is shown, using HPLC-MS-MS, that c-di-GMP copurifies with native VC2370. Mutation of this RXXD site prevents c-di-GMP copurification. Also, mutation of the site ensures that c-di-GMP produced during the in vitro reaction is not able to inhibit enzyme activity | Vibrio cholerae |
R242A | mutant sows activity and is used as a target enzyme. Mutation locks the enzyme in a constitutively active state | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4(R)-1-(benzylsulfonyl)-3-phenoxy-2-propanol | - |
Pseudomonas aeruginosa | |
4(R)-1-(benzylsulfonyl)-3-phenoxy-2-propanol | - |
Vibrio cholerae | |
4(S)-1-(benzylsulfonyl)-3-phenoxy-2-propanol | - |
Pseudomonas aeruginosa | |
4(S)-1-(benzylsulfonyl)-3-phenoxy-2-propanol | - |
Vibrio cholerae | |
N-(((2-phenylethyl)amino)carbonothioyl)benzamide | - |
Pseudomonas aeruginosa | |
N-(((2-phenylethyl)amino)carbonothioyl)benzamide | significantly reduces the total concentration of c-di-GMP in Vibrio cholerae | Vibrio cholerae | |
N-(4-anilinophenyl)benzamide | - |
Pseudomonas aeruginosa | |
N-(4-anilinophenyl)benzamide | significantly reduces the total concentration of c-di-GMP in Vibrio cholerae | Vibrio cholerae | |
N-(4-bromophenyl)-3-phenylpropanamide | - |
Pseudomonas aeruginosa | |
N-(4-bromophenyl)-3-phenylpropanamide | - |
Vibrio cholerae | |
N-(4-chlorophenyl)-3-phenylpropanamide | - |
Pseudomonas aeruginosa | |
N-(4-chlorophenyl)-3-phenylpropanamide | - |
Vibrio cholerae | |
N-(4-methoxyphenyl)-3-phenylpropanamide | - |
Pseudomonas aeruginosa | |
N-(4-methoxyphenyl)-3-phenylpropanamide | - |
Vibrio cholerae | |
N-(4-phenoxyphenyl)-2-thiophenecarboxamide | - |
Pseudomonas aeruginosa | |
N-(4-phenoxyphenyl)-2-thiophenecarboxamide | - |
Vibrio cholerae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Vibrio cholerae | Q9KPJ7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Pseudomonas aeruginosa | cyclic di-3',5'-guanylate + diphosphate | - |
? | |
GTP | - |
Vibrio cholerae | cyclic di-3',5'-guanylate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DGC | - |
Vibrio cholerae |
diguanylate cyclase | - |
Pseudomonas aeruginosa |
diguanylate cyclase | - |
Vibrio cholerae |
VC2370 | - |
Vibrio cholerae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Pseudomonas aeruginosa |
20 | - |
assay at | Vibrio cholerae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas aeruginosa |
7.5 | - |
assay at | Vibrio cholerae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0009 | - |
pH 7.5, 20°C | Vibrio cholerae | N-(((2-phenylethyl)amino)carbonothioyl)benzamide | |
0.001 | - |
pH 7.5, 20°C | Vibrio cholerae | N-(4-anilinophenyl)benzamide | |
0.0029 | - |
pH 7.5, 20°C | Vibrio cholerae | 4(R)-1-(benzylsulfonyl)-3-phenoxy-2-propanol | |
0.0066 | - |
pH 7.5, 20°C | Vibrio cholerae | N-(4-chlorophenyl)-3-phenylpropanamide | |
0.0069 | - |
pH 7.5, 20°C | Vibrio cholerae | N-(4-bromophenyl)-3-phenylpropanamide | |
0.008 | - |
pH 7.5, 20°C | Vibrio cholerae | N-(4-phenoxyphenyl)-2-thiophenecarboxamide | |
0.00817 | - |
pH 7.5, 20°C | Pseudomonas aeruginosa | 4(R)-1-(benzylsulfonyl)-3-phenoxy-2-propanol | |
0.0098 | - |
pH 7.5, 20°C | Vibrio cholerae | N-(4-methoxyphenyl)-3-phenylpropanamide | |
0.0122 | - |
pH 7.5, 20°C | Pseudomonas aeruginosa | N-(((2-phenylethyl)amino)carbonothioyl)benzamide | |
0.0135 | - |
pH 7.5, 20°C | Pseudomonas aeruginosa | N-(4-chlorophenyl)-3-phenylpropanamide | |
0.0175 | - |
pH 7.5, 20°C | Pseudomonas aeruginosa | N-(4-bromophenyl)-3-phenylpropanamide | |
0.0178 | - |
pH 7.5, 20°C | Pseudomonas aeruginosa | N-(4-anilinophenyl)benzamide | |
0.0189 | - |
pH 7.5, 20°C | Pseudomonas aeruginosa | N-(4-phenoxyphenyl)-2-thiophenecarboxamide | |
0.03 | - |
pH 7.5, 20°C | Pseudomonas aeruginosa | N-(4-methoxyphenyl)-3-phenylpropanamide |