BRENDA - Enzyme Database show
show all sequences of 2.8.3.5

Succinyl-CoA:3-ketoacid coenzyme A transferase (SCOT): development of an antibody to human SCOT and diagnostic use in hereditary SCOT deficiency

Song, X.Q.; Fukao, T.; Mitchell, G.A.; Kassovska-Bratinova, S.; Ugarte, M.; Wanders, R.J.; Hirayama, K.; Shintaku, H.; Churchill, P.; Watanabe, H.; Orii, T.; Kondo, N.; Biochim. Biophys. Acta 1360, 151-156 (1997)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Homo sapiens
General Stability
General Stability
Organism
enzyme is only soluble under denaturating conditions
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinyl-CoA + acetoacetate
Homo sapiens
enzyme deficiency leads to ketoacidotic crises and persistent ketosis
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
healthy and SCOT-deficient patients; SCOT-deficient patients
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fibroblast
-
Homo sapiens
-
lymphocyte
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinyl-CoA + acetoacetate
-
645991
Homo sapiens
succinate + acetoacetyl-CoA
-
-
-
?
succinyl-CoA + acetoacetate
enzyme deficiency leads to ketoacidotic crises and persistent ketosis
645991
Homo sapiens
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
General Stability (protein specific)
General Stability
Organism
enzyme is only soluble under denaturating conditions
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinyl-CoA + acetoacetate
Homo sapiens
enzyme deficiency leads to ketoacidotic crises and persistent ketosis
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fibroblast
-
Homo sapiens
-
lymphocyte
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinyl-CoA + acetoacetate
-
645991
Homo sapiens
succinate + acetoacetyl-CoA
-
-
-
?
succinyl-CoA + acetoacetate
enzyme deficiency leads to ketoacidotic crises and persistent ketosis
645991
Homo sapiens
?
-
-
-
-
Other publictions for EC 2.8.3.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737365
Zhang
Structure of succinyl-CoA:3-ke ...
Drosophila melanogaster
Acta Crystallogr. Sect. F
69
1089-1093
2013
-
-
1
1
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-
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1
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3
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1
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1
1
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1
1
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-
737420
Cong
Metallothionein prevents diabe ...
Mus musculus
Am. J. Physiol. Endocrinol. Metab.
304
E826-E835
2013
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1
1
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1
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1
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738807
Shafqat
A structural mapping of mutati ...
Homo sapiens
J. Inherit. Metab. Dis.
36
983-987
2013
-
-
1
1
22
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-
1
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-
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2
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1
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1
1
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1
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1
22
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1
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1
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-
1
1
-
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-
-
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-
-
-
738997
Chutoam
RpoS and oxidative stress cond ...
Burkholderia pseudomallei, Burkholderia pseudomallei K96243
Microbiol. Immunol.
57
605-615
2013
-
-
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4
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-
-
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-
-
-
724424
Fukao
Clinical and molecular charact ...
Homo sapiens
Biochim. Biophys. Acta
1812
619-624
2011
-
-
1
-
7
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
1
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1
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7
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-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
723826
Coker
The high-resolution structure ...
Sus scrofa
Acta Crystallogr. Sect. D
66
797-805
2010
-
-
-
1
-
-
-
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-
1
1
-
2
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1
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2
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1
1
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1
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1
1
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1
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2
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-
1
1
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724109
Hasan
Lower succinyl-CoA:3-ketoacid- ...
Rattus norvegicus
Arch. Biochem. Biophys.
499
62-68
2010
-
-
1
-
-
-
-
-
2
-
-
1
-
2
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-
-
-
3
-
-
1
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1
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2
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1
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3
-
-
1
-
-
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-
-
-
-
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-
1
1
-
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-
724277
Fraser
Catalytic role of the conforma ...
Sus scrofa
Biochemistry
49
10319-10328
2010
-
-
1
1
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
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-
-
1
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703384
MacDonald
Decreased levels of metabolic ...
Homo sapiens
Diabetologia
52
1087-1091
2009
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
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-
1
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-
-
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-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
703810
Bregere
Effects of age and calorie res ...
Rattus norvegicus
Free Radic. Biol. Med.
48
609-618
2009
-
-
-
-
-
-
-
-
2
-
2
1
-
3
-
1
1
-
-
2
1
-
2
2
1
-
-
-
1
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
3
-
4
1
-
-
1
2
-
3
1
-
2
2
1
-
-
-
1
-
-
2
1
3
3
1
-
-
705310
Skinner
Ketone bodies inhibit the viab ...
Homo sapiens
J. Pediatr. Surg.
44
212-216
2009
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
1
-
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-
-
1
-
1
-
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1
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-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
695186
Orii
Liver-specific silencing of th ...
Homo sapiens
Tohoku J. Exp. Med.
215
227-236
2008
-
1
-
-
-
-
1
-
1
-
-
2
-
2
-
-
-
-
-
3
-
-
3
-
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-
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-
1
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1
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-
1
-
-
2
-
-
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-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705523
Bregere
Detection and characterization ...
Rattus norvegicus
Methods Enzymol.
441
339-349
2008
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
2
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
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-
-
1
-
-
1
-
-
2
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672216
Tammam
Identification of the cysteine ...
Sus scrofa
Biochemistry
46
10852-10863
2007
-
1
1
1
4
-
11
11
1
1
-
4
-
4
-
-
1
1
-
2
4
-
6
2
1
-
-
6
1
-
-
-
-
-
-
-
1
2
-
2
6
-
-
11
-
22
1
1
-
4
-
-
-
2
-
3
7
-
6
2
1
-
-
6
1
-
-
-
-
-
-
-
-
-
676034
Yamada
Single-base substitution at th ...
Homo sapiens
Mol. Genet. Metab.
90
291-297
2007
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
1
-
2
-
-
-
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-
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-
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-
-
1
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-
1
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1
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-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676985
Stols
New vectors for co-expression ...
Bacillus subtilis
Protein Expr. Purif.
53
396-403
2007
-
-
1
1
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
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1
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-
-
1
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
690880
Rebrin
Nitration of tryptophan 372 in ...
Rattus norvegicus
Biochemistry
46
10130-10144
2007
-
-
-
-
-
-
-
-
2
-
2
3
-
2
-
-
1
-
-
9
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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2
-
2
3
-
-
-
1
-
9
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694193
Fukao
Identification and characteriz ...
Homo sapiens
Mol. Genet. Metab.
92
216-221
2007
-
1
-
-
1
1
-
-
1
-
-
3
-
2
-
-
-
-
-
1
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
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1
-
-
3
-
-
-
-
-
1
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
660674
El Midaoui
Physical training reverses def ...
Rattus norvegicus
Am. J. Physiol.
288
E748-752
2005
-
-
-
-
-
-
-
-
-
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1
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2
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1
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3
1
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1
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1
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1
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3
1
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1
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661256
Ohnuki
Different localization in rat ...
Rattus norvegicus
Biochim. Biophys. Acta
1729
147-153
2005
-
-
1
-
-
-
-
-
2
-
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1
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3
-
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-
2
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1
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1
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2
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1
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2
-
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1
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-
660636
Coros
Structure of the CoA transfera ...
Sus scrofa
ACTA CRYSTALLOGR. SECT. D
60
1717-1725
2004
-
-
-
1
1
-
-
-
-
2
-
1
-
2
-
-
-
-
-
1
-
-
1
2
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
-
-
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-
-
1
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663018
Fukao
Patients homozygous for the T4 ...
Homo sapiens
Pediatr. Res.
56
858-863
2004
-
1
1
-
3
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
1
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
1
1
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
645993
Tanaka
Cloning and characterization o ...
Homo sapiens
Mol. Hum. Reprod.
8
16-23
2002
-
-
1
-
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
1
-
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-
-
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-
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-
-
1
-
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1
-
1
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1
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-
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-
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-
-
-
645982
Berry
Neonatal hypoglycaemia in seve ...
Homo sapiens
J. Inherit. Metab. Dis.
24
587-595
2001
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
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-
1
1
-
1
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dimeric pig heart succinate-co ...
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Nitration of succinyl-CoA:3-ox ...
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Pig heart CoA transferase exis ...
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Succinyl-CoA:3-ketoacid CoA tr ...
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Productive interactions betwee ...
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Succinyl-CoA:3-ketoacid coenzy ...
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Succinyl CoA:3-oxoacid CoA tra ...
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Role of binding energy with co ...
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Purification and properties of ...
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Modification and inactivation ...
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Activity of 3-oxo acid CoA-tra ...
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The role of 3-oxo acid-CoA tra ...
Clupea harengus, Columba livia, Dicentrarchus labrax, Gallus gallus, Lacerta viridis, Mus musculus, Oncorhynchus mykiss, Oryctolagus cuniculus, Pleuronectes platessa, Raja clavata, Rattus norvegicus, Scomber scombrus, Scyliorhinus canicula
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Properties of succinyl-CoA:3-k ...
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Fenselau
Ping-pong chromatography. A no ...
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Fenselau
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Fenselau
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Biochemistry
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Edwards
A simple purification of aceto ...
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