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Literature summary for 3.1.1.13 extracted from
- Cytosolic cholesterol ester hydrolase from bovine corpus luteum. Its purification, identification, and relationship to hormone-sensitive lipase (1983), Biochim. Biophys. Acta, 752, 46-53.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cAMP-dependent protein kinase type II | 2fold activation in presence of ATP | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropylfluorophosphate | 50% inhibition at 0.005 mM | Bos taurus |  |
| Hg2+ | 50% inhibition at 0.01 mM | Bos taurus | |
| NaF | 50% inhibition at 3 mM | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Bos taurus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl esters + H2O | Bos taurus | supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues | cholesterol + fatty acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | phosphoprotein | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, 750fold, chromatography steps | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| corpus luteum | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.34 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 5 mM sodium phosphate, 30% glycerol w/v, 0.04% Triton X-100 w/v, 15 mM 2-mercaptoethanol, pH 7.0, several weeks | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl esters + H2O | supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues | Bos taurus | cholesterol + fatty acid | - |
? | |
| cholesteryl oleate + H2O | - |
Bos taurus | cholesterol + oleic acid | - |
? | |
| monooleoylalkylglycerol + H2O | - |
Bos taurus | alkylglycerol + oleic acid | - |
? | |
| triolein + H2O | - |
Bos taurus | dioleoylglycerol + oleic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 84000, SDS-PAGE | Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | in presence of cAMP-dependent protein kinase | Bos taurus | |
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Release 2023.1 (January 2023)
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