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Literature summary for 3.1.1.76 extracted from
- Production of chiral (R)-3-hydroxyoctanoic acid monomers, catalyzed by Pseudomonas fluorescens GK13 poly(3-hydroxyoctanoic acid) depolymerase (2010), Appl. Environ. Microbiol., 76, 3554-3560.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the activity of the pure enzyme is enhanced by the presence of detergents and organic solvents, and it is retained after treatment with an SDS-denaturing cocktail under both reducing and nonreducing conditions | Pseudomonas fluorescens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas fluorescens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
x * 26000, SDS-PAGE | Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(3-hydroxyoctanoic acid) + H2O | Pseudomonas fluorescens | the dimeric ester of 3-hydroxyoctanoic acid is obtained as the main product of the soluble enzyme | ? | - |
? |  |
| poly(3-hydroxyoctanoic acid) + H2O | Pseudomonas fluorescens GK13 | the dimeric ester of 3-hydroxyoctanoic acid is obtained as the main product of the soluble enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas fluorescens GK13 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| development of a rapid and easy immobilization method of the enzyme, the extracellular enzyme is tightly adsorbed onto a commercially available polypropylene support with high yield and specificity. The immobilized enzyme catalyzes almost the complete hydrolysis of poly(3-hydroxyoctanoic acid) polymer to (R)-3-hydroxyoctanoic acid monomers | Pseudomonas fluorescens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | strain GK13 is grown on mineral medium supplemented with poly(3-hydroxyoctanoic acid) as the sole carbon and energy source | Pseudomonas fluorescens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.4 | - |
substrate 4-nitrophenyl octanoate, pH 9.5, 40°C | Pseudomonas fluorescens |
| 76 | - |
substrate poly(3-hydroxyoctanoic acid), pH 9.5, 40°C | Pseudomonas fluorescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| the purified enzyme retains at least 75% of its original activity after 5 months of storage at -20 and -80°C, respectively | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl octanoate + H2O | - |
Pseudomonas fluorescens | octanoate + 4-nitrophenol | - |
? | |
| 4-nitrophenyl octanoate + H2O | - |
Pseudomonas fluorescens GK13 | octanoate + 4-nitrophenol | - |
? | |
| poly(3-hydroxyoctanoic acid) + H2O | the dimeric ester of 3-hydroxyoctanoic acid is obtained as the main product of the soluble enzyme | Pseudomonas fluorescens | ? | - |
? | |
| poly(3-hydroxyoctanoic acid) + H2O | the dimeric ester of 3-hydroxyoctanoic acid is obtained as the main product of the soluble enzyme | Pseudomonas fluorescens GK13 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 26000, SDS-PAGE | Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MCL-PHA depolymerase | - |
Pseudomonas fluorescens |
| medium-chain-length polyhydroxyalkanoate depolymerase | - |
Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pseudomonas fluorescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
relatively high stability at temperatures below 45°C | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Pseudomonas fluorescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 11 | over 60% of maximal activity within this range | Pseudomonas fluorescens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas fluorescens | peak 1, isoelectric focusing | - |
6.45 |
| Pseudomonas fluorescens | peak 2, isoelectric focusing | - |
6.95 |
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