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Literature summary for 3.4.22.B14 extracted from

  • Wojdyla, J.A.; Manolaridis, I.; van Kasteren, P.B.; Kikkert, M.; Snijder, E.J.; Gorbalenya, A.E.; Tucker, P.A.
    Papain-like protease 1 from transmissible gastroenteritis virus: Crystal structure and enzymatic activity toward viral and cellular substrates (2010), J. Virol., 84, 10063-10073.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3)pLysS cells Transmissible gastroenteritis virus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1-0.18 M (NH4)2SO4, 0.1 M MES (pH 6.5), and 30% (w/v) PEG 5000, at 19°C Transmissible gastroenteritis virus

Organism

Organism UniProt Comment Textmining
Transmissible gastroenteritis virus P0C6V2 46-MAD
-
Transmissible gastroenteritis virus Purdue P0C6V2 46-MAD
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Transmissible gastroenteritis virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-[[4-(dimethylamino)phenyl]azo]benzoic acid-MYNKMGGGDKTVSF-(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid) amide + H2O
-
 Transmissible gastroenteritis virus ?
-
 ?
4-[[4-(dimethylamino)phenyl]azo]benzoic acid-MYNKMGGGDKTVSF-(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid) amide + H2O
-
 Transmissible gastroenteritis virus Purdue ?
-
 ?
additional information PL1pro cleaves a peptide mimicking the cognate nsp2-nsp3 cleavage site, has deubiquitinating activity in an in vitro cleavage assay and displays a slight preference for Lys48-linked polyubiquitin chains over Lys63-linked ones Transmissible gastroenteritis virus ?
-
 ?
additional information PL1pro cleaves a peptide mimicking the cognate nsp2-nsp3 cleavage site, has deubiquitinating activity in an in vitro cleavage assay and displays a slight preference for Lys48-linked polyubiquitin chains over Lys63-linked ones Transmissible gastroenteritis virus Purdue ?
-
 ?
ORF 1a polyprotein + H2O
-
 Transmissible gastroenteritis virus ?
-
 ?
ORF 1a polyprotein + H2O
-
 Transmissible gastroenteritis virus Purdue ?
-
 ?
ubiquitin-7-amido-4-methylcoumarin + H2O
-
 Transmissible gastroenteritis virus ubiquitin + 7-amino-4-methylcoumarin
-
 ?
ubiquitin-7-amido-4-methylcoumarin + H2O
-
 Transmissible gastroenteritis virus Purdue ubiquitin + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
PL1pro
-
 Transmissible gastroenteritis virus
replicase polyprotein 1a
-
 Transmissible gastroenteritis virus