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Literature summary for 5.6.2.1 extracted from

  • Goto, T.; Laipis, P.; Wang, J.C.
    The purification and characterization of DNA topoisomerases I and II of the yeast Saccharomyces cerevisiae (1984), J. Biol. Chem., 259, 10422-10429.
    View publication on PubMed
Search for more literature for 5.6.2.1

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
 Saccharomyces cerevisiae
coumermycin
-
 Saccharomyces cerevisiae
Ethidium bromide
-
 Saccharomyces cerevisiae
additional information no inhibition: oxolinic acid Saccharomyces cerevisiae
Nalidixic acid no inhibition Saccharomyces cerevisiae
novobiocin
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Saccharomyces cerevisiae 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent cation is not required. 7-10 mM Ca2+ stimulates 10fold to 20fold Saccharomyces cerevisiae
Mg2+ divalent cation is not required. 7-10 mM Mg2+ stimulates 10fold to 20fold Saccharomyces cerevisiae
Mn2+ 25% as effective as Mg2+ in stimulation Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 1 * 90000, SDS-PAGE Saccharomyces cerevisiae
98000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
supercoiled DNA relaxation Saccharomyces cerevisiae relaxed closed circular DNA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 90000, SDS-PAGE Saccharomyces cerevisiae