Cloned (Comment) | Organism |
---|---|
expression of FLAG-tagged K681A mutant enzyme in Saccharomyces cerevisiae | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K681A | site-directed mutagenesis, the mutation of Lys681, located on the tip of the linker domain, dramatically reduces the religation rate, the equilibrium of the mutant is shifted toward cleavage when compared to the wild-type enzyme. Mutation of residue 681 from lysine to alanine does not cause alterations in the linker domain secondary structure, and the main chain conformation of the mutated residue does not significantly change in relation with the neighbor residues, when compared to the wild-type protein | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | DNA topoisomerase IB catalyzes the relaxation of supercoiled DNA through the transient cleavage of one strand of a DNA duplex, molecular dynamics simulation of wild-type and mutant K681A enzymes, the N-terminal and C-terminal regions of the core domain show a slightly larger and a slightly smaller displacements, respectively, in the wild type than in the mutant enzyme, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | DNA topoisomerase IB catalyzes the relaxation of supercoiled DNA through the transient cleavage of one strand of a DNA duplex, molecular dynamics simulation of wild-type and mutant K681A enzymes, the N-terminal and C-terminal regions of the core domain show a slightly larger and a slightly smaller displacements, respectively, in the wild type than in the mutant enzyme, overview | Homo sapiens | ? | - |
? | |
additional information | cleavage of oligonucleotide substrates 5'-GAAAAAAGACTTAG-3' and 5'-TAAAAATTTTTCTAAGTCTTTTTTC-3'. Crucial role for the linker domain and occurrence of a long range communication between the linker and the active site region, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA topoisomerase IB | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
cleavage assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
cleavage assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is of fundamental importance to processes such as replication, recombination and transcription | Homo sapiens |