EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.1.1.85 | -999 |
- |
more |
Michaelis-Menten kinetics of recombinant wild-type and mutant enzymes at different temperatures, overview |
701046 |
1.1.1.85 | -999 |
- |
more |
the Km-value for NAD+ ranges from 0.054 mM to 0.15 mM when enzyme concentration varies from 0.008 mg/ml to 0.16 mg/ml, the Km-value for 3-isopropylmalate ranges from 0.023 mM to 0.042 mM when enzyme concentration varies from 0.008 mg/ml to 0.16 mg/ml |
639134, 639147 |
1.1.1.85 | -999 |
- |
more |
thermodynamic and kinetics analysis, overview |
696778 |
1.1.1.85 | 0.000017 |
- |
isopropylmalate |
pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme |
639155 |
1.1.1.85 | 0.00048 |
- |
isopropylmalate |
pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87G |
639155 |
1.1.1.85 | 0.001 |
- |
isopropylmalate |
pH 8.0, 40°C, mutant enzyme C275T |
639156 |
1.1.1.85 | 0.0011 |
- |
D-3-ethylmalate |
pH 8.0 |
680429 |
1.1.1.85 | 0.0012 |
- |
D-3-isopropylmalate |
pH 8.0 |
680429 |
1.1.1.85 | 0.0012 |
- |
isopropylmalate |
pH 8.0, 40°C, mutant enzyme G376A |
639156 |
1.1.1.85 | 0.00125 |
- |
isopropylmalate |
pH 8.0, 40°C, wild-type enzyme |
639156 |