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Information on EC 1.1.1.85 - 3-isopropylmalate dehydrogenase
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EC Tree
1.1.1.85 3-isopropylmalate dehydrogenaseIUBMB Comments
The product decarboxylates spontaneously to yield 4-methyl-2-oxopentanoate.
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Word Map
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
3-isopropylmalate dehydrogenase, ipmdh, beta-isopropylmalate dehydrogenase, isopropylmalate dehydrogenase, ipmdh2, ipmdh3, beta-ipm dehydrogenase, sbipmdh, ipmdh1, 3-ipm dehydrogenase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2-hydroxy-4-methyl-3-carboxyvalerate:NAD+ oxidoreductase
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2R,3S-isopropylmalate:NAD+ oxidoreductase (decaboxylating)
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3-IPM dehydrogenase
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3-IPM-DH
3-isopropylmalate dehydrogenase
beta-IPM dehydrogenase
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beta-IPMDH
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beta-isopropylmalate dehydrogenase
beta-isopropylmalic enzyme
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dehydrogenase, 3-isopropylmalate
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IMDH
IPMDH
IPMDH1
IPMDH2
IPMDH3
isopropylmalate dehydrogenase
leuB
NAD-dependent isopropylmalate dehydrogenase
Saci_0600
threo-Ds-3-isopropylmalate dehydrogenase
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two-domain 3-isopropylmalate dehydrogenase
3-IPM-DH
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beta-isopropylmalate dehydrogenase
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IMDH
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IPMDH
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IPMDH
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isopropylmalate dehydrogenase
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NAD-dependent isopropylmalate dehydrogenase
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NAD-dependent isopropylmalate dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(2R,3S)-3-isopropylmalate + NAD+ = (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
the oxidative decarboxylation of 2-hydroxy acid substrates by the enzyme occurs via a random kinetic mechanism with the formation of two abortive complexes: enzyme-NADH-substrate and enzyme-NADH-inhibitor
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(2R,3S)-3-isopropylmalate + NAD+ = (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
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SYSTEMATIC NAME
IUBMB Comments
(2R,3S)-3-isopropylmalate:NAD+ oxidoreductase
The product decarboxylates spontaneously to yield 4-methyl-2-oxopentanoate.
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CAS REGISTRY NUMBER
COMMENTARY
9030-97-1
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2R,3S)-3-isopropylmalate + NAD+
2-ketoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxovalerate + CO2 + NADH + H+
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?
(2R,3S)-3-isopropylmalate + thionicotinamide NAD+
2-oxoisohexanoate + thionicotinamideNADH + CO2
(S)-malate + NAD+
pyruvate + NADH + CO2
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12% of the activity with 3-isopropylmalate
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?
3-(2'-methylthio)ethylmalate + NAD+
? + NADH + H+
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?
3-isoamylmalate + NAD+
3-isoamylpyruvate + NADH + CO2
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?
3-tert-butylmalate + NAD+
3-tert-butylpyruvate + NADH + CO2
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?
beta-methylmalate + NAD+
2-oxobutyrate + NADH + CO2
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enzyme catalyzes the final step in the pyruvate pathway to 2-oxobutyrate
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?
D-3-methylmalate + NAD+
D-3-methyl-2-oxosuccinate + NADH + H+
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?
D-3-t-butylmalate + NAD+
D-3-t-butyl-2-oxosuccinate + NADH + H+
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?
DL-3-isopropylmalate + NAD+
DL-isopropyl-3-oxosuccinate + NADH + H+
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?
tert-butylmalate + NAD+
2-hydroxy-4,4-dimethylpentanoate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
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-
-
-
r
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
-
-
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
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-
-
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
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-
-
-
r
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
-
-
-
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
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-
-
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
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-
-
-
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
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-
-
-
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
-
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?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Shewanella oneidensis MR-1 / ATCC 700550
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?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
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?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
-
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?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
-
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r
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
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?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
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?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
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?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
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?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
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?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
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?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
mutant enzymes with increased activity
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?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
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?
(2R,3S)-3-isopropylmalate + NAD+
2-isopropyl-3-oxosuccinate + NADH + H+
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r
(2R,3S)-3-isopropylmalate + NAD+
2-isopropyl-3-oxosuccinate + NADH + H+
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r
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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a large movement of domain 1 is induced by substrate binding, which results in the formation of the hydrophobic pocket for the gamma-isopropyl moiety of isopropylmalate
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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key enzyme in leucine biosynthesis
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
Bacillus sp. (in: Bacteria) no 38-2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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-
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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-
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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erythro-Lg-beta-isopropylmalate
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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the enzyme catalyzes the third pathway-specific reaction in the biosynthesis of leucine
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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penultimate step in leucine biosynthesis
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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enzyme is involved in leucine biosynthesis
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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639130, 639131, 639136, 639138, 639139, 639140, 639146, 639152, 639153, 639155, 639156, 639157, 639158, 639159, 639160, 639164, 639165, 639167, 724976
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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decarboxylation appears to take place with retention of configuration
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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enzyme of leucine biosynthesis
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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key enzyme in leucine biosynthesis
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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-
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?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
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?
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
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ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
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ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
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ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
the enzyme belongs to the leucine biosynthetic pathway
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ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
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ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
the enzyme belongs to the leucine biosynthetic pathway
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ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
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ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
-
-
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
-
-
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
-
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ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
-
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?
(2R,3S)-3-isopropylmalate + NAD+
?
the enzyme is enzyme involved in leucine biosynthesis
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?
(2R,3S)-3-isopropylmalate + NAD+
D-3-isopropyl-2-oxosuccinate + NADH + H+
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?
(2R,3S)-3-isopropylmalate + NAD+
D-3-isopropyl-2-oxosuccinate + NADH + H+
-
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?
(2R,3S)-3-isopropylmalate + thionicotinamide NAD+
2-oxoisohexanoate + thionicotinamideNADH + CO2
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?
(2R,3S)-3-isopropylmalate + thionicotinamide NAD+
2-oxoisohexanoate + thionicotinamideNADH + CO2
-
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?
3-ethylmalate + NAD+
3-ethylpyruvate + NADH + CO2
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DL-erythro-beta-ethylmalate
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?
3-isopropylmalate + NAD+
2-ketoisocaproate + CO2 + NADH + H+
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the enzyme catalyzes a step in leucine biosynthesis
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?
D-3-ethylmalate + NAD+
D-3-ethyl-2-oxosuccinate + NADH + H+
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-
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?
D-3-ethylmalate + NAD+
D-3-ethyl-2-oxosuccinate + NADH + H+
-
-
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?
D-3-isobutylmalate + NAD+
D-3-isobutyl-2-oxosuccinate + NADH + H+
-
-
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?
D-3-isobutylmalate + NAD+
D-3-isobutyl-2-oxosuccinate + NADH + H+
-
-
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?
D-3-isopentylmalate + NAD+
D-3-isopentyl-2-oxosuccinate + NADH + H+
-
-
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?
D-3-isopentylmalate + NAD+
D-3-isopentyl-2-oxosuccinate + NADH + H+
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?
D-malate + NAD+
pyruvate + NADH + CO2
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19% of the activity with 3-isopropylmalate
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?
threo-D-3-isopropylmalate + NAD+
?
domain 1 binds the coenzyme NAD+, while the substrate 3-isopropylmalate binds mainly to domain 2
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?
threo-D-3-isopropylmalate + NAD+
?
domain 1 binds the coenzyme NAD+, while the substrate 3-isopropylmalate binds mainly to domain 2
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?
threo-D-3-isopropylmalate + NAD+
?
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domain 1 binds the coenzyme NAD+, while the substrate 3-isopropylmalate binds mainly to domain 2
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?
threo-D-3-isopropylmalate + NAD+
?
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domain 1 binds the coenzyme NAD+, while the substrate 3-isopropylmalate binds mainly to domain 2
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?
threo-DL-isopropylmalate + NAD+
2-isopropyl-3-oxosuccinate + NADH
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?
threo-DL-isopropylmalate + NAD+
2-isopropyl-3-oxosuccinate + NADH
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?
additional information
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substrate specificity-determining residues, overview
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additional information
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substrate specificity-determining residues, overview
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additional information
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substrate specificity-determining residues, overview
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additional information
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substrate specificity-determining residues, overview
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additional information
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substrate specificity-determining residues, overview
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additional information
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substrate specificity-determining residues, overview
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additional information
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substrate specificity-determining residues, overview
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additional information