EC Number   |
|---|
   2.7.7.65 | application of membrane-free extract on GTP-agarose column |
| 2.7.7.65 | by Ni-NTA affinity chromatography, furthermore a Superdex 200 and a gel filtration column are used |
| 2.7.7.65 | from bacterial lysate by metal affinity chromatography and gel-filtration (size-exclusion chromatography), product cyclic di-3,5-guanylate co-purified with wild-type and mutants L170D and L167D from bacterial extracts despite dialysis, nucleotide-free wild-type generated by treatment with phosphodiesterase and subsequent metal affinity chromatography and gel-filtration |
| 2.7.7.65 | from bacterial lysate by metal affinity chromatography and gel-filtration, product cyclic di-3,5-guanylate co-purified with wild-type from bacterial extracts despite dialysis, nucleotide-free wild-type generated by treatment with phosphodiesterase and subsequent metal affinity chromatography and gel-filtration |
| 2.7.7.65 | from bacterial lysate by metal affinity chromatography using HisTrap HP column and FPLC (elution: linear imidazole gradient) and dialysis |
| 2.7.7.65 | immobilized metal affinity chromatography followed by dialysis and analytical or preparative size exclusion chromatography on Superdex 200 column |
| 2.7.7.65 | metal affinity chromatography (elution with 200 mM imidazole) followed by size-exclusion chromatography on Superdex 200 HR 26/60 column and analytical size-exclusion chromatography on Superdex 200 HR 10/30 column |
| 2.7.7.65 | on a Sephadex G25 and a DEAE-Sepharose column, furthermore an amylose-resin matrix is used |
| 2.7.7.65 | recombinant C-terminally His6-tagged CdgH periplasmic portion |
| 2.7.7.65 | recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, the tag is cleaved off by PreScission protease |
