EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   2.4.2.48 | guanine15 in tRNA + 7-cyano-7-deazaguanine |
- |
Methanosarcina acetivorans |
7-cyano-7-deazaguanine15 in tRNA + guanine |
- |
? |
| 2.4.2.48 | guanine15 in tRNA + 7-cyano-7-deazaguanine |
- |
Thermoplasma acidophilum HO-62 |
7-cyano-7-deazaguanine15 in tRNA + guanine |
- |
? |
| 2.4.2.48 | guanine15 in tRNA(Leu) + 7-cyano-7-carbaguanine |
- |
Pyrococcus horikoshii |
7-cyano-7-carbaguanine15 in tRNA(Leu) + guanine |
- |
? |
| 2.4.2.48 | guanine15 in tRNA(Leu) + 7-cyano-7-carbaguanine |
- |
Thermoplasma acidophilum |
7-cyano-7-carbaguanine15 in tRNA(Leu) + guanine |
- |
? |
| 2.4.2.48 | guanine15 in tRNA(Leu) + 7-cyano-7-carbaguanine |
- |
Pyrococcus horikoshii OT-3 |
7-cyano-7-carbaguanine15 in tRNA(Leu) + guanine |
- |
? |
| 2.4.2.48 | more |
the enzyme catalyzes a transglycosylation reaction in which guanine is eliminated from position 15 of the tRNA and an archaeosine precursor (preQ0) is inserted. The enzyme is able to utilize both guanine and the 7-deazaguanine base preQ0 as substrates, but not other 7-deazaguanine bases, and is able to modify tRNA from all three phylogenetic domains. The enzyme shows good activity with the tRNASer transcript as well as tRNA from Escherichia coli and yeast |
Methanocaldococcus jannaschii |
? |
- |
? |
| 2.4.2.48 | tRNAguanine + 7-cyano-7-deazaguanine |
archaeal TGT substitutes 7-cyano-7-deazaguanine for the G in position 15 of tRNA as the first step in archaeosine formation |
Pyrococcus furiosus |
? |
- |
? |
