EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.3.1.110 | lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster |
lactate is a common substrate for major groups of strictly anaerobic bacteria. The LDH/Etf complex of Acetobacterium woodii uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD+ as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD+ |
Acetobacterium woodii |
pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster |
- |
r |
1.3.1.110 | lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster |
pyruvate reduction to lactate with NADH as electron donor is highly exergonic |
Acetobacterium woodii |
pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster |
- |
r |
1.3.1.110 | lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster |
lactate is a common substrate for major groups of strictly anaerobic bacteria. The LDH/Etf complex of Acetobacterium woodii uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD+ as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD+ |
Acetobacterium woodii DSM 1030 |
pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster |
- |
r |
1.3.1.110 | lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster |
pyruvate reduction to lactate with NADH as electron donor is highly exergonic |
Acetobacterium woodii DSM 1030 |
pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster |
- |
r |
1.3.1.110 | lactate + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster |
- |
Acetobacterium woodii |
pyruvate + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster |
- |
? |
1.3.1.110 | pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster |
no NADH oxidation with pyruvate as electron acceptor. Only when oxidized ferredoxin is added to the system, the reaction is initiated and NADH oxidation is detected by a decrease of absorbance. NADH-dependent ferredoxin reduction is strictly dependent on the presence of lactate |
Acetobacterium woodii |
lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster |
- |
r |
1.3.1.110 | pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster |
no NADH oxidation with pyruvate as electron acceptor. Only when oxidized ferredoxin is added to the system, the reaction is initiated and NADH oxidation is detected by a decrease of absorbance. NADH-dependent ferredoxin reduction is strictly dependent on the presence of lactate |
Acetobacterium woodii DSM 1030 |
lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster |
- |
r |