EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.1.1.41 | -999 |
- |
thermal denaturation is an irreversible process |
727835 |
1.1.1.41 | 0 |
- |
full loss in activity in a few hours |
286766 |
1.1.1.41 | 28 |
35 |
the enzyme is stable below 28°C, but rapidly loses activity above 30°C, and 60% activity remains after a 20 min incubation at 35°C |
761311 |
1.1.1.41 | 33 |
- |
purified recombinant enzyme, 20 min, loss of 50% activity |
741236 |
1.1.1.41 | 38 |
- |
purified recombinant enzyme, 20 min, loss of over 90% activity |
741236 |
1.1.1.41 | 40 |
- |
purified recombinant enzyme, stable up to, rapid inactivation above |
723607 |
1.1.1.41 | 40 |
- |
purified recombinant His-tagged enzyme, rapid inactivation above |
722295 |
1.1.1.41 | 40 |
47 |
the enzyme is stable below 40°C and half of its activity is lost after incubation at 47°C for 20 min |
760747 |
1.1.1.41 | 40 |
55 |
recombinant CaIDH retains the majority of the activity below 45°C, its activity drops rapidly as the temperature is raised. Incubation at 55°C for 20 min causes a 55% or 75% loss of activity in the presence of Mg2+ or Mn2+, respectively |
741414 |
1.1.1.41 | 42 |
- |
10 min, 50% loss in activity |
286773 |