EC Number |
Cofactor |
Reference |
---|
4.1.1.7 | more |
the addition of thiamine thiazolone diphosphate has no effect on the activity of BFD in the presence of thiamine diphosphate |
678577 |
4.1.1.7 | thiamine diphosphate |
- |
677679, 691346, 691794, 702289, 702290, 702341, 714480, 714487, 716121, 747071, 747171, 747467, 747471, 747599 |
4.1.1.7 | thiamine diphosphate |
0.5 mM, required for activity |
694892 |
4.1.1.7 | thiamine diphosphate |
dependent |
678422, 679209, 679212, 679656, 680368, 680979, 682628 |
4.1.1.7 | thiamine diphosphate |
dependent on |
4800, 4806, 4807, 4810, 666806, 691020, 702796, 715697 |
4.1.1.7 | thiamine diphosphate |
dependent on, structural binding environment, overview. The V-conformation is assisted by the presence of a fulcrum residue, Leu403, located directly below the cofactor, the V-conformation results in a N4'-C2 distance of 3.1 A |
727483 |
4.1.1.7 | thiamine diphosphate |
Km: 0.001 mM |
4807 |
4.1.1.7 | thiamine diphosphate |
required, Km: 0.001 mM |
678577 |
4.1.1.7 | thiamine diphosphate |
the 4'-aminopyrimidine ring of the thiamine diphosphate coenzyme participates in catalysis, likely as an intramolecular general acid-base catalyst via the unusual 1',4'-iminopyrimidine tautomer |
678222 |
4.1.1.7 | thiamine diphosphate |
the enzyme avoids formation of a benzoyl anion by addition of enzyme-bound thiamine diphosphate to the carbonyl group of benzoylformate, producing enzyme-bound 2-(2-mandelyl)thiamine diphosphate |
680352 |