EC Number   |
Protein Variants |
Reference |
|---|
   1.5.3.10 | Y259F |
able to oxidize substrate, steady-state turnover is attenuated 200fold, rate of FAD reduction is substantially impaired, no stabilization of FADH2-iminium charge-transfer complex |
672060 |
| 1.5.3.10 | more |
complete removal of 5,10-CH2-THF synthase domain through expression of a truncated version generates a mutant enzyme unable to avoid hydrolysis of the imine species generated following amine oxidation |
698911 |
| 1.5.3.10 | H225Q |
modest changes in kinetic parameters, no stabilization of FADH2-iminium charge-transfer complex |
672060 |
| 1.5.3.10 | D552A |
mutation leads to increased formaldehyde release |
698911 |
| 1.5.3.10 | H225Q |
site-directed mutagenesis, reduced catalytic activity compared to the wild-type enzyme |
655336 |
| 1.5.3.10 | Y259F |
site-directed mutagenesis, reduced catalytic activity compared to the wild-type enzyme |
655336 |
