EC Number   |
Protein Variants |
Reference |
|---|
    3.6.1.54 | D117A |
2fold decrease in activity compared to wild-type |
-, 734230 |
| 3.6.1.54 | D42A |
80000fold decrease in activity compared to wild-type |
-, 734230 |
| 3.6.1.54 | D9A |
200000fold decrease in activity compared to wild-type |
734230 |
| 3.6.1.54 | F141H/L142S/L146S/F147H/E14A/E15A/K161T/E162A |
solubilized enzyme version with 488% activity compared to the wild type enzyme, including four solubilizing mutations (F141H, L142S, L146S, and F147H) and four surface entropy reduction mutations (E14A, E15A, K161T, and E162A) to improve protein expression and crystallization |
757216 |
| 3.6.1.54 | H10N |
the inactive mutant shows loss of manganese 1-binding ability |
758374 |
| 3.6.1.54 | H115A |
12000fold decrease in activity compared to wild-type |
-, 734230 |
| 3.6.1.54 | H11A |
20000fold decrease in activity compared to wild-type |
-, 734230 |
| 3.6.1.54 | H196A |
5000fold decrease in activity compared to wild-type |
-, 734230 |
| 3.6.1.54 | I47C/R149C |
the mutant shows 141% activity compared to the wild type enzyme |
757216 |
| 3.6.1.54 | I47G/R149G |
the mutant shows 11.3% activity compared to the wild type enzyme |
757216 |
